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Sperm Cryopreservation in Crustaceans

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Cryopreservation of Fish Gametes

Abstract

Sperm cryopreservation facilities and reproductive management adds flexibility to breeding program design; it also allows mobilization over long distances, extends sperm half-life for several years, enables the use of selected males and prevent the reduction of genetic variability caused by inbreeding or environmental disasters. Crustaceans hold over 30% value of the aquaculture market, despite its commercial importance, procedures granting its genetic diversity protection are not fully developed or widespread. To date, few papers have been reported on the cryopreservation of sperm from crustaceans, this is due to the diverse morphology between sperm and spermatophore among crustacean species, together with its different fertilization strategies. To assess the viability of sperm, it is common to use the microscopic examination of membrane integrity. However, the detailed reproductive characteristics of crustaceans have not been fully explained and not all interactions between their sperm and cryoprotectants are known. Therefore, the “gold test” to verify the viability of cryopreserved/thawed sperm is to test its fertilization rate.

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Correspondence to Carmen Guadalupe Paniagua-Chávez .

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Morales-Ueno, K., Paniagua-Chávez, C.G. (2020). Sperm Cryopreservation in Crustaceans. In: Betsy, J., Kumar, S. (eds) Cryopreservation of Fish Gametes. Springer, Singapore. https://doi.org/10.1007/978-981-15-4025-7_10

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