Abstract
Throughout the world, more people suffer from malnutrition than hunger, particularly in developing countries. Some nutrients like iodine, vitamin A, iron, and zinc malnutrition are significant concerns. Biofortification is the most resilient method to improve the nutrient content of the crop plants and is a durable and cost-effective method of introducing genes to overcome the nutrient deficiencies faced by the people in developing countries. Currently, agronomic, conventional, and transgenic biofortification are three common approaches to nutrient biofortification. In this chapter, the significant progress made in transgenic biofortification development, their applicability, and future challenges has been discussed. The transgenic approach has been utilized for the successful development of crops to acquire the nutrients that do not exist naturally. Recently, several reports on the development of transgenic crops to enhance levels of essential micronutrient contents in crops like tomato, sweet potato, potato, beans, cassava, and other vegetable crops have been reported.
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References
Al-Babili S, Beyer P (2005) Golden Rice–five years on the road–five years to go? Trends Plant Sci 10(12):565–573
Aragão FJL, Barros LMG, De Sousa MV, Grossi de Sá MF, Almeida ERP, Gander ES, Rech EL (1999) Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa HBK, Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae). Genet Mol Biol 22(3):445–449
Arimond M, Ruel MT (2004) Dietary diversity is associated with child nutritional status: evidence from 11 demographic and health surveys. J Nutr 134(10):2579–2585
Bailey DP, Broom DR, Chrismas BC, Taylor L, Flynn E, Hough J (2015) Breaking up prolonged sitting time with walking does not affect appetite or gut hormone concentrations but does induce an energy deficit and suppresses postprandial glycaemia in sedentary adults. Appl Physiol Nutr Metab 41(3):324–331
Banakar R, Alvarez Fernandez A, Díaz-Benito P, Abadia J, Capell T, Christou P (2017) Phytosiderophores determine thresholds for iron and zinc accumulation in biofortified rice endosperm while inhibiting the accumulation of cadmium. J Exp Bot 68(17):4983–4995
Black RE, Bhutta ZA LHA, Caulfield LE, De Onnis M, Ezzati M, Mathers C, Rivera J (2008) Maternal and child undernutrition: global and regional exposures and health consequences. Lancet 371:243–260
Boonyaves K, Gruissem W, Bhullar NK (2016) NOD promoter-controlled AtIRT1 expression functions synergistically with NAS and FERRITIN genes to increase iron in rice grains. Plant Mol Biol 90(3):207–215
Boonyaves K, Wu TY, Gruissem W, Bhullar NK (2017) Enhanced grain iron levels in rice expressing an iron-regulated metal transporter, nicotianamine synthase, and ferritin gene cassette. Front Plant Sci 8:130
Borg S, Brinch-Pedersen H, Tauris B, Madsen LH, Darbani B, Noeparvar S, Holm PB (2012) Wheat ferritins: improving the iron content of the wheat grain. J Cereal Sci 56(2):204–213
Bouis HE (2003) Micronutrient fortification of plants through plant breeding: can it improve nutrition in man at low cost? Proc Nutr Soc 62(2):403–411
Bouis HE (1999) Economics of enhanced micronutrient density in food staples. Field Crop Res 60:165–173. https://doi.org/10.1016/S0378-4290(98)00138-5
Bouis H, Howarth E (2000) Enrichment of food staples through plant breeding: a new strategy for fighting micronutrient malnutrition. Nutrition (Burbank, Los Angeles County, Calif) 16(7–8):701–704
Bouis HE, Chassy BM, Ochanda JO (2003) 2. Genetically modified food crops and their contribution to human nutrition and food quality. Trends Food Sci Technol 14(5–8):191–209
Bouis HE, Hotz C, McClafferty B, Meenakshi JV, Pfeiffer WH (2011) Biofortification: a new tool to reduce micronutrient malnutrition. Food Nutr Bull 32(1 Suppl):S31–S40
Brinch-Pedersen H, Borg S, Tauris B, Holm PB (2007) Molecular genetic approaches to increasing mineral availability and vitamin content of cereals. J Cereal Sci 46(3):308–326
Burkhardt PK, Beyer P, Wünn J, Klöti A, Armstrong GA, Schledz M, von Lintig J, Potrykus I (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J 11(5):1071–1078
Chaudhary J, Khatri P, Singla P, Kumawat S, Kumari A, Vikram A, Jindal SK, Kardile H, Kumar R, Sonah H (2019) Advances in omics approaches for abiotic stress tolerance in tomato. Biology 8(4):90
Chizuru N, Ricardo U, Shiriki K, Prakash S (2003) The joint WHO/FAO expert consultation on diet, nutrition and the prevention of chronic diseases: process, product and policy implications. Public Health Nutr 7(1a):245–250. https://doi.org/10.1079/PHN2003592
Christou P, Twyman RM (2004) The potential of genetically enhanced plants to address food insecurity. Nutr Res Rev 17(1):23–42
De Lepeleire J, Strobbe S, Verstraete J, Blancquaert D, Ambach L, Visser RG, Stove C, Van Der Straeten D (2018) Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes. Mol Plant 11(1):175–188
De Onis M, Branca F (2016) Childhood stunting: a global perspective. Matern Child Nutr 12:12–26
Diretto G, Tavazza R, Welsch R, Pizzichini D, Mourgues F, Papacchioli V, Beyer P, Giuliano G (2006) Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. BMC Plant Biol 6(1):13
Distelfeld A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Budak H, Saranga Y, Fahima T (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiol Plant 129(3):635–643
Doshi KM, Eudes F, Laroche A, Gaudet D (2006) Transient embryo-specific expression of anthocyanin in wheat. Vitro Cell Dev Biol-Plant 42(5):432–438
Ducreux LJ, Morris WL, Hedley PE, Shepherd T, Davies HV, Millam S, Taylor MA (2004) Metabolic engineering of high carotenoid potato tubers containing enhanced levels of β-carotene and lutein. J Exp Bot 56(409):81–89
Fanzo J. The nutrition challenge in sub-Saharan Africa (No. 2012–012). United Nations Development Programme, Regional Bureau for Africa; 2012
FAO (2013) FAO statistical yearbook: world food and agriculture
FAO (2015) IFAD, and Unicef. "WFP." The state of food insecurity in the world 46
Gaitán-Solís E, Taylor NJ, Siritunga D, Stevens W, Schachtman DP (2015) Overexpression of the transporters AtZIP1 and AtMTP1 in cassava changes zinc accumulation and partitioning. Front Plant Sci 6:492
Goto F, Yoshihara T, Saiki H (2000) Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theor Appl Genet 100(5):658–664
Graham H, Peter H, Vance CP (2003) Legumes: importance and constraints to greater use. Plant Physiol 131(3):872–877
Gupta HS, Agrawal PK, Mahajan V, Bisht GS, Kumar A, Verma P, Srivastava A, Saha S, Babu R, Pant MC, Mani VP (2009) Quality protein maize for nutritional security: rapid development of short duration hybrids through molecular marker assisted breeding. Curr Sci 96(2):230–237
Hefferon KL (2015) Nutritionally enhanced food crops; progress and perspectives. Int J Mol Sci 16(2):3895–3914
Hirschi A (2009) Career adaptability development in adolescence: multiple predictors and effect on sense of power and life satisfaction. J Vocat Behav 74(2):145–155
Hong H, Datla N, Reed DW, Covello PS, MacKenzie SL, Qiu X (2002) High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare. Plant Physiol 129(1):354–362
Howell EL, Wirz CD, Brossard D, Jamieson KH, Scheufele DA, Winneg KM, Xenos MA (2018) National Academies of Sciences, Engineering, and Medicine report on genetically engineered crops influences public discourse. Politics Life Sci 37(2):250–261
Hurrell R, Egli I (2010) Iron bioavailability and dietary reference values. Am J Clin Nutr 91(5):1461S–1467S
Ihemere U, Narayanan N, Sayre R (2012) Iron biofortification and homeostasis in transgenic cassava roots expressing an algal iron assimilatory protein, FEA1. Front Plant Sci 3:171
Inaba M, Macer D (2004) Policy, regulation and attitudes towards agricultural biotechnology in Japan. J Int Biotechnol Law 1(2):45–53
Initiative M (2009) Investing in the future: a united call to action on vitamin and mineral deficiencies. Micronutrient Initiative, Ottawa
Lee S, An G (2009) Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice. Plant Cell Environ 32(4):408–416
Li L, Paolillo DJ, Parthasarathy MV, DiMuzio EM, Garvin DF (2001) A novel gene mutation that confers abnormal patterns of β-carotene accumulation in cauliflower (Brassica oleracea var. botrytis). Plant J 26(1):59–67
Lu S, Van Eck J, Zhou X, Lopez AB, O’Halloran DM, Cosman KM, Conlin BJ, Paolillo DJ, Garvin DF, Vrebalov J, Kochian LV, Küpper H, Earle ED, Cao J, Li L (2006) The cauliflower Or gene encodes a DnaJ cysteine-rich domain-containing protein that mediates high levels of β-carotene accumulation. Plant Cell 18(12):3594–3605
Lyons G, Cakmak I (2012) Agronomic biofortification of food crops with micronutrients. Fertilizing crops to improve human health: a scientific review. Food Nutr Secur 1:97–122
Marles RJ (2017) Mineral nutrient composition of vegetables, fruits and grains: the context of reports of apparent historical declines. J Food Compos Anal 56:93–103
Masuda H, Ishimaru Y, Aung MS, Kobayashi T, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Nishizawa NK (2012) Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Sci Rep 2:543
Masuda H, Aung MS, Nishizawa NK (2013) Iron biofortification of rice using different transgenic approaches. Rice 6(1):40
McGuire S (2015) FAO, IFAD, and WFP. The state of food insecurity in the world 2015: meeting the 2015 international hunger targets: taking stock of uneven progress. Rome: FAO, 2015. Adv Nutr 6(5):623–624. https://doi.org/10.3945/an.115.009936
Moura MDG, Lopes LC, Biavatti MW, Busse JW, Wang L, Kennedy SA, Bhatnaga N, de Cássia Bergamaschi C (2016) Brazilian oral herbal medication for osteoarthritis: a systematic review protocol. Syst Rev 5(1):86
Mushtaq M, Mukhtar S, Sakina A, Dar AA, Bhat R, Deshmukh R, Molla K, Kundoo AA, Dar MS (2020) Tweaking genome-editing approaches for virus interference in crop plants. Plant Physiol Biochem 147:8
Muthusamy V, Hossain F, Thirunavukkarasu N, Choudhary M, Saha S, Bhat JS, Prasanna BM, Gupta HS (2014) Development of β-carotene rich maize hybrids through marker-assisted introgression of β-carotene hydroxylase allele. PLoS One 9(12):e113583
Nestel P, Bouis HE, Meenakshi JV, Pfeiffer W (2006) Biofortification of staple food crops. J Nutr 136(4):1064–1067
Newell-McGloughlin M (2008) Nutritionally improved agricultural crops. Plant Physiol 147(3):939–953
Nicolia A, Manzo A, Veronesi F, Rosellini D (2014) An overview of the last 10 years of genetically engineered crop safety research. Crit Rev Biotechnol 34(1):77–88
Oliva N, Chadha-Mohanty P, Poletti S, Abrigo E, Atienza G, Torrizo L, Garcia R, Dueñas C Jr, Poncio MA, Balindong J, Manzanilla M, Montecillo F, Zaidem M, Barry G, Hervé P, Shou H, Slamet-Loedin IH (2014) Large-scale production and evaluation of marker-free indica rice IR64 expressing phytoferritin genes. Mol Breed 33(1):23–37
Paul S, Ali N, Datta SK, Datta K (2014) Development of an iron-enriched high-yieldings indica rice cultivar by introgression of a high-iron trait from transgenic iron-biofortified rice. Plant Foods Hum Nutr 69(3):203–208
Park HS, Rene ER, Choi SM, Chiu AS (2008) Strategies for sustainable development of industrial park in Ulsan, South Korea—from spontaneous evolution to systematic expansion of industrial symbiosis. J Environ Manag 87(1):1–13
Pérez-Massot E, Banakar R, Gómez-Galera S, Zorrilla-López U, Sanahuja G, Arjó G, Miralpeix B, Vamvaka E, Farré G, Rivera SM, Dashevskaya S, Berman J, Sabalza M, Yuan D, Bai C, Bassie L, Twyman RM, Capell T, Christou P, Zhu C (2013) The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. Genes Nutr 8(1):29
Petry N, Olofin I, Hurrell R, Boy E, Wirth J, Moursi M, Donahue Angel M, Rohner F (2016) The proportion of anemia associated with iron deficiency in low, medium, and high human development index countries: a systematic analysis of national surveys. Nutrients 8(11):693
Pfeiffer WH, McClafferty B (2007) HarvestPlus: breeding crops for better nutrition. Crop Sci 47(S3):S88–S105
Pierce EC, LaFayette PR, Ortega MA, Joyce BL, Kopsell DA, Parrott WA (2015) Ketocarotenoid production in soybean seeds through metabolic engineering. PLoS One 10(9):e0138196
Qaim M, Stein AJ, Meenakshi JV (2007) Economics of biofortification. Agric Econ 37:119–133
Rana N, Rahim MS, Kaur G, Bansal R, Kumawat S, Roy J, Deshmukh R, Sonah H, Sharma TR (2019) Applications and challenges for efficient exploration of omics interventions for the enhancement of nutritional quality in rice (Oryza sativa L.). Crit Rev Food Sci Nutr:1–17. https://doi.org/10.1080/10408398.2019.1685454
Rodríguez-Celma J, Schmidt W (2013) Reduction-based iron uptake revisited: on the role of secreted iron-binding compounds. Plant Signal Behav 8(11):1473–1485
Saltzman A, Birol E, Bouis HE, Boy E, De Moura FF, Islam Y, Pfeiffer WH (2013) Biofortification: progress toward a more nourishing future. Glob Food Sec 2(1):9–17
Sperotto RA, Ricachenevsky FK (2017) Common bean Fe biofortification using model species’ lessons. Front Plant Sci 8:2187
Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, Lambert W, Van Der Straeten D (2007) Folate fortification of rice by metabolic engineering. Nat Biotechnol 25(11):1277
Tan S, Han R, Li P, Yang G, Li S, Zhang P, Wang WB, Zhao WZ, Yin LP (2015) Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds. Transgenic Res 24(1):109–122
Trijatmiko KR, Dueñas C, Tsakirpaloglou N, Torrizo L, Arines FM, Adeva C, Balindong J, Oliva N, Sapasap MV, Borrero J, Rey J, Francisco P, Nelson A, Nakanishi H, Lombi E, Tako E, Glahn RP, Stangoulis J, Chadha-Mohanty P, Johnson AA, Tohme J, Barry G, Slamet-Loedin IH (2016) Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Sci Rep 6:19792
Tsuda M, Watanabe KN, Ohsawa R (2019) Regulatory status of genome-edited organisms under the Japanese Cartagena Act. Front Bioeng Biotechnol 7:387
Vats S, Kumawat S, Kumar V, Patil GB, Joshi T, Sonah H, Sharma TR, Deshmukh R (2019) Genome editing in plants: exploration of technological advancements and challenges. Cell 8(11):1386
Voogt W, Holwerda HT, Khodabaks R (2010) Biofortification of lettuce (Lactuca sativa L.) with iodine: the effect of iodine form and concentration in the nutrient solution on growth, development and iodine uptake of lettuce grown in water culture. J Sci Food Agric 90(5):906–913
Wang C, Zeng J, Li Y, Hu W, Chen L, Miao Y, Deng P, Yuan C, Ma C, Chen X, Zang M, Wang Q, Li K, Chang J, Wang Y, Yang G, He G (2014) Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI. J Exp Bot 65(9):2545–2556
Wesseler J, Zilberman D (2014) The economic power of the golden rice opposition. Environ Dev Econ 19(6):724–742
White PJ, Broadley MR (2005) Biofortifying crops with essential mineral elements. Trends Plant Sci 10(12):586–593
Wirth J, Poletti S, Aeschlimann B, Yakandawala N, Drosse B, Osorio S, Tohge T, Fernie AR, Günther D, Gruissem W, Sautter C (2009) Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin. Plant Biotechnol J 7(7):631–644
Xiaoyan S, Yan Z, Shubin W (2012) Improvement Fe content of wheat (Triticum aestivum) grain by soybean ferritin expression cassette without vector backbone sequence. J Agric Biotechnol 20:766–773
Yang QQ, Zhang CQ, Chan ML, Zhao DS, Chen JZ, Wang Q, Li QF, Yu HX, Gu MH, Sun SS, Liu QQ (2016) Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance. J Exp Bot 67(14):4285–4296
Ye X, Al-Babili S, Klöti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287(5451):303–305
Zhang L, Yang XD, Zhang YY, Yang J, Qi GX, Guo DQ, Xing GJ, Yao Y, Xu WJ, Li HY, Li QY, Dong YS (2014) Changes in oleic acid content of transgenic soybeans by antisense RNA mediated posttranscriptional gene silencing. Int J Genom 2014:921950
Zhu C, Sanahuja G, Yuan D, Farré G, Arjó G, Berman J, Zorrilla-López U, Banakar R, Bai C, Pérez-Massot E, Bassie L, Capell T, Christou P (2013) Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies. Plant Biotechnol J 11(2):129–141
Zou T, Xu N, Hu G, Pang J, Xu H (2014) Biofortification of soybean sprouts with zinc and bioaccessibility of zinc in the sprouts. J Sci Food Agric 94(14):3053–3060
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Mir, Z.A. et al. (2020). Transgenic Biofortified Crops: Applicability and Challenges. In: Sharma, T.R., Deshmukh, R., Sonah, H. (eds) Advances in Agri-Food Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-15-2874-3_7
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