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Application of Fluorescence in Studying Therapeutic Enzymes

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Therapeutic Enzymes: Function and Clinical Implications

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1148))

Abstract

Fluorescence spectroscopy is one of the most important techniques in the study of therapeutic enzymes. The fluorescence phenomenon has been discovered and exploited for centuries, while therapeutic enzymes have been used in treatment of disease for only decades. This chapter provides a brief summary of the current applications of fluorescence methods in studying therapeutic enzymes to provide some insights on the selection of proper method tailored to the goal. First a brief introduction about therapeutic enzymes and history of fluorescence were provided, followed by discussions on how fluorescence was applied in the studies. Four popular fluorescence methods are discussed: fluorescence tracing, fluorescence resonance energy transfer (FRET), fluorescence quenching and fluorescence polarization. Selected application of the fluorescence methods in studying therapeutic enzymes are listed, and discussed in details in the following paragraphs.

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Abbreviations

FRET:

Fluorescence resonance energy transfer

MS:

Mass spectroscopy

CD:

Circular dichroism

FTIR:

Fourier-transform infrared spectroscopy

US-FDA:

Food and Drug Administration in USA

SCID:

Severe combined immunodeficiency disease

mAbs:

Monoclonal antibodies

IgG1:

Immunoglobulin G1

GFP:

Green Fluorescent Protein

GBA:

Glucocerebrosidase

ABP:

Activity-based probes

MCL:

Mantle cell lymphoma

MESK:

Microencapsulated streptokinase

FREE SK:

Unencapsulated streptokinase

CFP:

Cyan fluorescent protein

YFP:

Yellow fluorescent protein

Abl:

Ableson

Bcr:

Break point cluster

ECFP:

Enhanced cyan fluorescent protein

HPMA:

N-(2-hydroxypropyl) methacrylamide

ADA:

Adenosine deaminase

FAM:

6-carboxyfluo rescein

MWCNTs:

Multi-walled carbon nanotubes

IgE:

Immunoglobulin E

FcεRI:

The IgE Fc receptor

IFN-γ:

Interferon gamma

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Science, University of Kentucky, Lexington, KY, USA

    Zhaoshuai Wang

  2. Department of Educational, School, and Counseling Psychology, University of Kentucky, Lexington, KY, USA

    Caihong Li

  3. Department of Chemistry, University of Kentucky, Lexington, KY, USA

    Yinan Wei

Authors
  1. Zhaoshuai Wang
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  2. Caihong Li
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  3. Yinan Wei
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Corresponding author

Correspondence to Zhaoshuai Wang .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos Labrou

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Wang, Z., Li, C., Wei, Y. (2019). Application of Fluorescence in Studying Therapeutic Enzymes. In: Labrou, N. (eds) Therapeutic Enzymes: Function and Clinical Implications. Advances in Experimental Medicine and Biology, vol 1148. Springer, Singapore. https://doi.org/10.1007/978-981-13-7709-9_5

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