Abstract
In vitro antimicrobial assessment of natural extracts or pure compounds involves the use of assays to evaluate their potential to kill or inhibit microbial growth in a laboratory setting. Several methods have been developed for this purpose, which include Agar Well Diffusion and Disc Diffusion methods, Dynamic Contact assay, Thin-Layer Chromatography-Bioautography, Time-Kill assay and Biofilm assays, Microdilution methods and broth assays for Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Doubling Time/Growth Curve (MDT), Flow Cytofluorometric Assay and Bioluminescence Assay. This chapter will provide the readers a brief but comprehensive methodology of these in-vitro assays along with their advantages and disadvantages. It will serve as a guide in choosing the most appropriate antimicrobial assay for their research objectives.
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Kurhekar, J., Tupas, G.D., Otero, M.C.B. (2019). In-vitro Assays for Antimicrobial Assessment. In: Kumar, S., Egbuna, C. (eds) Phytochemistry: An in-silico and in-vitro Update. Springer, Singapore. https://doi.org/10.1007/978-981-13-6920-9_15
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DOI: https://doi.org/10.1007/978-981-13-6920-9_15
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