Abstract
Cell is the smallest unit in both plants and animals which continuously communicate with each other or with their environment. Cells have evolved a variety of signaling mechanism involving a network of events which is required to transmit the signals. This whole process is an intricate meshwork of interactions involving the crossroads of chemistry, physics, and biology. Over the course of a hundred million years of evolution, scientists have invented a number of biochemical approaches to explore these millions of biological networks both in vitro (in single isolated cells) and in vivo (in an intact organism) in plants and animals. Some of the in vitro methods developed so far have been talked about in the chapter. Nevertheless, despite technical improvements in the global understanding of signal transduction, its internal structures and its highly integrated and extremely dynamic nature remains largely mysterious and efforts should be made to invent some more rapid and improved methods to better our understanding of signal transduction.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bari R, Jones JD. Role of plant hormones in plant defence responses. Plant Mol Biol. 2009;69:473.
Berrier AL, Mastrangelo AM, Downward J, Ginsberg M, LaFlamme S. Activated R-Ras, Rac1, PI 3-kinase and PKC [small element of] can each restore cell spreading inhibited by isolated integrin b1 cytoplasmic domains. J Cell Biol. 2000;151:1549–60.
Depuydt S, Hardtke CS. Hormone signalling crosstalk in plant growth regulation. Curr Biol. 2011;21:R365–73.
ESTs Factsheet. National Center for Biotechnology Information.
Fraire-Velázquez S, RodrÃguez-Guerra R, Sánchez-Calderón L. Abiotic and biotic stress response crosstalk in plants. In: Shanker A, editor. Abiotic stress response in plants—physiological, biochemical and genetic perspectives. Rijeka: InTech; 2011. p. 3–26.
Harvey L, Arnold B, S Lawrence Z, Paul M, David B, James D. Molecular Cell Biology. 4th ed. 2000.
Hauser F, Waadt R, Schroeder JI. Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol. 2011;21:R346–55.
Jaspers P, Kangasjärvi J. Reactive oxygen species in abiotic stress signaling. Physiol Plant. 2010;138:405–13.
Leyser O. Auxin, self-organisation and the colonial nature of plants. Curr Biol. 2011;21:R331–7.
Lukowitz W, Gillmor CS, Scheible WR. Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you. Plant Physiol. 2000;23:795–805.
Marques MC, Alonso-Cantabrana H, Forment H, et al. A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus. BMC Genomics. 2009;10:428.
Matyus L. Fluorescence resonance energy transfer measurements on cell surfaces A spectroscopic tool for determining protein interactions. J Photochem Photobiol B. 1992;12:323–37.
Michaelson D, Silletti J, Murphy G, D’Eustachio P, Rush M, Philips MR. Differential localization of Rho GRPases in live cells: regulation by hypervariable regions and RhoGDI binding. J Cell Biol. 2001;152:111–26.
Ossowski L, Aguirre-Ghiso JA. Urokinase receptor and integrin partnership: coordination of signaling for cell adhesion, migration and growth. Curr Opin Cell Biol. 2000;12:613–20.
Peleman JD, van der Voort JR. Breeding by design. Trends Plant Sci. 2003;8:330–4.
Pérez-Alfocea F, Ghanem ME, Gómez-Cadenas A, Dodd I. Omics of root-to-shoot signaling under salt stress and water deficit. OMICs J Integr Biol. 2011;15:893–901.
Porter JC, Hogg N. Integrins take partners: cross-talk between integrins and other membrane receptors. Trends Cell Biol. 1998;8:390–6.
Santner A, Estelle M. Recent advances and emerging trends in plant hormone signalling. Nature. 2009;459:1071–8.
Sastry SK, Burridge K. Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics. Exp Cell Res. 2000;261:25–36.
Schwartz MA, Baron V. Interactions between mitogenic stimuli, or a thousand and one connections. Curr Opin Cell Biol. 1999;11:197–202.
Schwartz MA, Shattil SJ. Signaling networks linking integrins and rho family GTPases. Trends Biochem Sci. 2000;25:388–91.
Sun TP. The molecular mechanism and evolution of the GA–GID1– DELLA signaling module in plants. Curr Biol. 2011;21:R338–45.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Jaiswal, N. (2019). In-vitro Techniques to Study Cell Signaling. In: Kumar, S., Egbuna, C. (eds) Phytochemistry: An in-silico and in-vitro Update. Springer, Singapore. https://doi.org/10.1007/978-981-13-6920-9_14
Download citation
DOI: https://doi.org/10.1007/978-981-13-6920-9_14
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-6919-3
Online ISBN: 978-981-13-6920-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)