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Isolation of Microfibrillated Cellulose (MFC) Via Fungal Cellulases Hydrolysis Combined with Ultrasonication

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Multifaceted Protocol in Biotechnology

Abstract

Celluloses, the most abundant biopolymers which exist in plant sources are potential materials to be used in manufacturing high performance composites due to theirs fascinating structure and properties. In plant cell walls, they are embedded in matrix substances such as hemicellulose and lignin. Removal of matrix substances is required before fibrillating the cellulose fibres into nanoscale-sized. The obtained microfibrillated cellulose (MFC) poses different features depending to its origin and degree of initial processing procedures and fibrillation method use. Thus, this chapter explains the isolation steps of microfibril cellulose (MFC) from cocoa pod husk via fungal cellulases hydrolysis combined with ultrasonication. The morphology and strutural observation of the MFC of cocoa pod husk (CPH) via scanning electron microscopy images and fourier transform infrared spectroscopy analysis respectively were also showed in this chapter.

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Correspondence to Dzun Noraini Jimat .

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Jimat, D.N., Aviceena (2018). Isolation of Microfibrillated Cellulose (MFC) Via Fungal Cellulases Hydrolysis Combined with Ultrasonication. In: Amid, A., Sulaiman, S., Jimat, D., Azmin, N. (eds) Multifaceted Protocol in Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-13-2257-0_10

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