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Molecular Genomic Techniques for Identification of Soil Microbial Community Structure and Dynamics

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Advances in Soil Microbiology: Recent Trends and Future Prospects

Part of the book series: Microorganisms for Sustainability ((MICRO,volume 3))

Abstract

Soil microorganisms play a crucial role in maintaining major biogeochemical/nutrient cycle, soil quality, and productivity. Hence, the understanding of soil microbial community structure, distribution, and their metabolic function is essential for getting a deeper insight into soil ecosystem and its health. A number of molecular methods for extracting metagenome, total RNA, protein, and metabolites from the diverse environmental samples, sequencing technology, etc. are present which help to know about microbial structure, composition, and their metabolic function in the specific environmental ecosystem. Genetic fingerprinting like ARDRA, RFLP, DGGE, and T-RFLP and omics approaches like metagenomics, metatranscriptomics, and metabolomics are essential techniques for identifying and depicting the total microbial community structure and their interactions with environmental and biotic factors. So for these molecular techniques, it is possible to identify and functionally characterize soil microbes that are not culturable in a laboratory environment. This chapter describes old and modern novel state of the art molecular techniques which proved insights into the phylogenetic and functional activities of microbial assemblages in a terrestrial ecosystem.

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Authors and Affiliations

  1. Microbial Culture Collection, National Centre for Cell Science, Savitribai Phule University of Pune Campus, Ganeshkhind, Pune, Maharashtra, India

    Dhiraj Paul, Satish Kumar, Mrinal Mishra, Sushant Parab, Sunil Banskar & Yogesh S. Shouche

  2. ICAR-National Institute of Abiotic Stress Management, Baramati Pune, Maharashtra, India

    Satish Kumar

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  3. Mrinal Mishra
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  4. Sushant Parab
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  5. Sunil Banskar
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  6. Yogesh S. Shouche
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Corresponding authors

Correspondence to Dhiraj Paul or Yogesh S. Shouche .

Editor information

Editors and Affiliations

  1. KIIT School of Biotechnology, Bhubaneswar, Odisha, India

    Tapan Kumar Adhya

  2. Environmental & Industrial Biotechnology, The Energy & Resources Institute (TERI), New Delhi, India

    Banwari Lal

  3. Environmental Microbiology Laboratory, Indian Institute of Technology, Kharagpur, West Bengal, India

    Balaram Mohapatra

  4. Microbial Ecology Laboratory, National Centre for Cell Science, Pune, Maharashtra, India

    Dhiraj Paul

  5. Environmental & Industrial Biotechnology, The Energy & Resources Institute (TERI), New Delhi, Delhi, India

    Subhasis Das

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Paul, D., Kumar, S., Mishra, M., Parab, S., Banskar, S., Shouche, Y.S. (2018). Molecular Genomic Techniques for Identification of Soil Microbial Community Structure and Dynamics. In: Adhya, T., Lal, B., Mohapatra, B., Paul, D., Das, S. (eds) Advances in Soil Microbiology: Recent Trends and Future Prospects. Microorganisms for Sustainability, vol 3. Springer, Singapore. https://doi.org/10.1007/978-981-10-6178-3_2

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