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Identification Strategies for Bioactive Secondary Metabolites of Fungal Origin

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Medicinal Plants and Fungi: Recent Advances in Research and Development

Part of the book series: Medicinal and Aromatic Plants of the World ((MAPW,volume 4))

Abstract

In the present review article, we have described several fungi as a promising and evolving source of new and bioactive secondary metabolites for drug discovery processes. Also, the review has outlined isolation and identification strategies of known and novel fungal metabolites from endophytic fungi, fungi isolated from marine sources and extreme environments, etc. The chapter highlights how recent advances in biochemistry, genetics, analytical technologies and bioinformatics have significantly influenced the process of discovery of novel bioactive compounds in fungi. Combination of microfractionation, high-resolution mass spectroscopy and liquid chromatography with emerging technologies such as microfluidics has potential to augment the efficiency of identification procedures for novel bioactive molecules and become effective tools for bioengineers to adapt these microorganisms for industrial applications.

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Abbreviations

CIEIA:

Competitive inhibition enzyme immunoassay

DAD:

Diode array detector

ECD:

Electrochemical detector

ELS:

Evaporative light scattering

FDA:

Food and drug administration

FT-IR:

Fourier transform infrared spectroscopy

HPLC:

High-performance liquid chromatography

HRESIMS:

High-resolution electrospray ionisation mass spectroscopy

HTS:

High-throughput screening

IR:

Infrared

LC-MS:

Liquid chromatography-mass spectroscopy

MIC:

Minimum inhibitory concentration

MS:

Mass spectroscopy

NMR:

Nuclear magnetic resonance spectroscopy

PCR:

Polymerase chain reaction

PDA:

Photodiode array

QTOF:

Quadrupole time of flight

SPE:

Solid-phase extraction

TLC:

Thin-layer chromatography

UHPLC:

Ultrahigh-performance liquid chromatography

UV:

Ultraviolet

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Authors and Affiliations

  1. Faculty of Engineering, Bioengineering Department, Ege University, Bornova, İzmir, Turkey

    İsmail Hakkı Akgün & Fazilet Vardar-Sukan

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  1. İsmail Hakkı Akgün
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  2. Fazilet Vardar-Sukan
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Correspondence to İsmail Hakkı Akgün .

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Editors and Affiliations

  1. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan

    Dinesh Chandra Agrawal

  2. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan

    Hsin-Sheng Tsay

  3. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan

    Lie-Fen Shyur

  4. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan

    Yang-Chang Wu

  5. Department of Forestry, National Chung Hsing University, Taichung, Taiwan

    Sheng-Yang Wang

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Akgün, İ.H., Vardar-Sukan, F. (2017). Identification Strategies for Bioactive Secondary Metabolites of Fungal Origin. In: Agrawal, D., Tsay, HS., Shyur, LF., Wu, YC., Wang, SY. (eds) Medicinal Plants and Fungi: Recent Advances in Research and Development. Medicinal and Aromatic Plants of the World, vol 4. Springer, Singapore. https://doi.org/10.1007/978-981-10-5978-0_16

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