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Photoirradiation and Microwave Irradiation NMR Spectroscopy

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Experimental Approaches of NMR Spectroscopy

Abstract

In situ photoirradiation solid-state nuclear magnetic resonance (NMR) spectroscopy is designed for optical irradiation from the top part of a zirconia rotor through a glass cap, which makes it possible to efficiently irradiate the inside of the rotor. This experimental method has made it possible to observe photo-intermediates of sensory rhodopsins, such as sensory rhodopsin I (SRI) and sensory rhodopsin II (SRII), and bacteriorhodopsin (bR) Y185F mutant. In SRI, green light generates M-intermediates, which exhibit positive phototaxis, while blue light generates P-intermediates, which exhibit negative phototaxis. In SRII, green light generates M-intermediates and blue light generates O-intermediates. In Y185F-bR, O-intermediates were first observed using solid-state NMR spectroscopy. The microwave irradiation NMR spectrometer was developed in-house by modification of a commercial NMR spectrometer. A flat long copper ribbon was used as a capacitor and a half turn of copper ribbon at the edge was used as an inductor for the microwave resonance circuit, which was coaxially inserted inside the radiofrequency induction coil and allowed NMR signals to be observed under microwave irradiation conditions. The temperature of N-(4-methoxybenzylidene)-4-butylaniline (MBBA) during microwave irradiation was estimated by measuring the temperature-dependent chemical shifts, whereby different protons were found to indicate significantly different temperatures in the molecule. Liquid crystalline-isotropic phase correlation 2D NMR spectra were observed using pulsed microwave irradiation for rapid temperature jump experiments.

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Acknowledgements

This work was supported by grants-in-aid for Scientific Research in an Innovative Area (16H00756 to AN and 16H00828 to IK), and by a grant-in-aid for Scientific Research (C) (15K06963 to AN) and Research (B) (15H04336 to IK) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Faculty of Engineering, Yokohama National University, 79-5 Hodogayaku Tokiwadai, Yokohama, 240-8501, Japan

    Akira Naito & Izuru Kawamura

  2. Graduate School of Engineering, Yokohama National University, 79-5 Hodogayaku Tokiwadai, Yokohama, 240-8501, Japan

    Yoshiteru Makino & Yugo Tasei

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  1. Akira Naito
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  2. Yoshiteru Makino
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  3. Yugo Tasei
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  4. Izuru Kawamura
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Corresponding author

Correspondence to Akira Naito .

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  1. Kobe, Japan

    The Nuclear Magnetic Resonance Society of Japan

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Naito, A., Makino, Y., Tasei, Y., Kawamura, I. (2018). Photoirradiation and Microwave Irradiation NMR Spectroscopy. In: The Nuclear Magnetic Resonance Society of Japan (eds) Experimental Approaches of NMR Spectroscopy. Springer, Singapore. https://doi.org/10.1007/978-981-10-5966-7_5

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