Abstract
Pseudomonas fluorescens NCIM 2100 was studied for its potential to produce extracellular biosurfactant in the nutrient broth medium supplemented with diesel (2%) as an additional carbon source. Biosurfactant production was checked based on oil spread and drop collapse test. Emulsification index test was conducted to determine the emulsifying ability of the crude biosurfactant produced. The emulsification index, minimum surface tension and production of crude bioemulsifier were found to be 90.38% with diesel, 34.40 ± 0.03 mN/m and 2.80 ± 0.45 g/L respectively. Maximum product accumulation occurred during stationary phase of the bacterial growth curve. Crude biosurfactant produced was stable, withstanding a wide temperature (37–80 °C) and pH range (7–10.5), with an E-24 Index value greater than 50%. Produced biosurfactant has an efficient emulsifying ability. Most of the emulsions formed with tested aliphatic hydrocarbons and ester-based vegetable oils were stable. Emulsions formed with different hydrophobic substrates were oil-in-water (o/w) in nature.
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Acknowledgements
Neha Panjiar acknowledges Council of Scientific and Industrial Research (CSIR), New Delhi for the research fellowship provided [09/554(0023)/2010-EMR-I] and corresponding authors acknowledge the University Grant Commission (UGC), Government of India for the financial support [F. No. 40-160/2011(SR)]. The authors are grateful to Central Instrumentation Facility (CIF) and Department of Bio-Engineering at Birla Institute of Technology, Mesra, Ranchi for providing culture and instrumentation facilities necessary to carry out the work.
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Panjiar, N., Sachan, S.G., Sachan, A. (2017). Biosurfactant Production by Pseudomonas fluorescens NCIM 2100 Forming Stable Oil-in-Water Emulsions. In: Mukhopadhyay, K., Sachan, A., Kumar, M. (eds) Applications of Biotechnology for Sustainable Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-5538-6_13
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DOI: https://doi.org/10.1007/978-981-10-5538-6_13
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