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Transmembrane Receptor Dynamics as Biophysical Markers for Assessing Cancer Cells

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Handbook of Single Cell Technologies

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Abstract

As biophysical properties of tissues and cells play essential roles in cellular function, morphogenesis, and disease progression, numerous conventional techniques have been developed to characterize cells and differentiate benign cells from cancer cells. Recently, Transmembrane Receptor Dynamics, a single-particle tracking based biophysical phenotyping assay, was developed as a contact-free technique that can probe the topography of the plasma membrane and nanostructure of the membrane-associated cytoskeleton with sub-diffraction-limited resolution. Here, we review this state-of-the-art technology by narrating the underlying biophysical principles of single-particle tracking and discussing the interpretation of the dynamics of tyrosine kinase receptors. We further elaborate its potential for gaining insights into biology and being translated as a cancer diagnostic tool on the basis of machine learning models.

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Acknowledgments

We thank Dr. Hsin-Chih Yeh for sharing his experience of SPT/SMT techniques. Soonwoo Hong for insightful discussions and feedback on the application of deep-learning in the trajectory analysis. This work was funded by the Ministry of Science and Technology, Taiwan (MOST 108-2636-E-039-001) and China Medical University, Taiwan (CMU108-YTY-01).

Author Contributions

M. Kim and Y.-L. Liu wrote the manuscript.

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Authors and Affiliations

  1. Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA

    Mirae Kim

  2. Master Program for Biomedical Engineering, China Medical University, Taichung, Taiwan

    Yen-Liang Liu

  3. Research Center for Cancer Biology, China Medical University, Taichung, Taiwan

    Yen-Liang Liu

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  1. Mirae Kim
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  2. Yen-Liang Liu
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Correspondence to Yen-Liang Liu .

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Editors and Affiliations

  1. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Tuhin Subhra Santra

  2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan

    Fan-Gang Tseng

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Kim, M., Liu, YL. (2021). Transmembrane Receptor Dynamics as Biophysical Markers for Assessing Cancer Cells. In: Santra, T.S., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_38-1

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