Abstract
Point-of-care devices are highly desired not only in developing countries, but also in developed countries for the medical detection for remote/rural areas and home use. Compared with conventional bulky immunosystem widely used in central laboratories and hospitals, point-of-care device is usually cost-effective for each test, with small footprint and light weight, and however tends to suffer low sensitivity and inaccurate quantification. So some signal amplification mechanisms are required for the development of a reliable point-of-care device with high sensitivity for clinical use, such as early-stage cancer detections. In this book chapter, we introduce in detail for the development of point-of-care devices based on gold nanoarray generated plasmonics, i.e., localized surface plasmon resonance (LSPR). We have demonstrated that the gold nanoarray sensing chip can be fabricated in a cost-effective way by combining the nickel mold fabrication and nanoimprinting, and the sensing chip can be used to detect the clinical related biomarkers either by direct one-step assay through the LSPR absorption peak shift detected using a portable optical spectroscopy, with example given for cardiac troponin I (cTnI); or by a sandwich assay with its fluorescent label signal amplified by LSPR to achieve higher sensitivity, which can be detected on a commercial microscope or home-developed fully automated point-of-care device, as exemplified by prostate specific antigen (PSA) detection for prostate cancer, thrombin detection for blood clots, and procalcitonin (PCT) detection for sepsis. The advantage of the gold nanoarray LSPR-based point-of-care device is that the sensing area can be as small as the light beam, the sensing chip can be sealed in a microfluidic channel and use a little amount of the analyte and bio-reagent, and the optical signal can be detected by images to facilitate the future multichannel detections with little additional cost. Our fully automated point-of-care device detects the PCT with only 50 μL of analyte, and provides the data with 30 min at a test cost of less than 10 US dollars. Our experiments also demonstrated that both kinds of point-of-care devices are with enough sensitivity for clinical use.
References
Altissimo M (2010) E-beam lithography for micro−/nanofabrication. Biomicrofluidics 4:026503
Anker JN, Hall WP, Lyandres O, Shah NC, Zhao J, Van Duyne RP (2008) Biosensing with plasmonic nanosensors. Nat Mater 7:442–453
Austin MD, Ge H, Wu W, Li M, Yu Z, Wasserman D, Lyon SA, Chou SY (2004) Fabrication of 5 nm linewidth and 14 nm pitch features by nanoimprint lithography. Appl Phys Lett 84(26):5299–5301
Chen Y (2015) Nanofabrication by electron beam lithography and its applications: a review. Microelectron Eng 135:57–72
Chirouze C, Schuhmacher H, Rabaud C, Gil H, Khayat N, Estavoyer JM, May T, Hoen B (2002) Low serum procalcitonin level accurately predicts the absence of bacteremia in adult patients with acute fever. Clin Infect Dis 35:156–161
Deng J, Wong TI, Sun LL, Quan C, Zhou X (2016) Acceleration of e-beam lithography by minimized resist exposure for large scale nanofabrication. Microelectron Eng 166:31–38
Ding T, Hong M, Richards AM, Wong TI, Zhou X, Drum C (2015) Quantification of a cardiac biomarker in human serum using localized surface plasmon resonance. PLoS One 10(3):e0120974
Ghaemi HF, Thio T, Grupp DE, Ebbesen TW, Lezec HJ (1998) Surface plasmons enhance optical transmission through subwavelength holes. Phys Rev B 58(11):6779–6782
Juan ML, Righini M, Quidant R (2011) Plasmon nano-optical tweezers. Nat Photonics 5:349–356
Kopterides P, Siempos II, Tsangaris I, Tsantes A, Armaganidis A (2010) Procalcitonin-guided algorithms of antibiotic therapy in the intensive care unit: a systematic review and meta-analysis of randomized controlled trials. Crit Care Med 38(11):2229–2241
Kosaka PM, González S, Domínguez CM, Cebollada A, San Paulo A, Calleja M, Tamayo J (2013) Atomic force microscopy reveals two phases in single stranded DNA self-assembled monolayers. Nanoscale 5:7425–7432
Kuwata H, Tamaru H, Esumi K, Miyano K (2003) Resonant, light scattering from metal nanoparticles: practical analysis beyond Rayleigh approximation. Appl Phys Lett 83:4625
Lee K-S, El-Sayed MA (2006) Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape, and metal composition. J Phys Chem B 110(39):19220–19225
Mayer KM, Hafner JH (2011) Localized surface plasmon resonance sensors. Chem Rev 111:3828–3857
Murphy CJ, Sau TK, Gole AM, Orendorff CJ, Gao J, Gou L, Hunyadi SE, Li T (2005) Anisotropic metal nanoparticles: synthesis, assembly, and optical applications. J Phys Chem B 109:13857–13870
Palik E (1998) Handbook of optical constants of solids. Elsevier, New York
Rai-Choudhury P (ed) (1997) Handbook of microlithography, micromachining and microfabrication. IET, Hertfordshire, p 349
Sherry LJ, Chang S-H, Schatz GC, Van Duyne RP, Wiley BJ, Xia Y (2005) Localized surface plasmon resonance spectroscopy of single silver nanocubes. Nano Lett 5(10):2034–2038
Song HY, Hobley J, XD S, Zhou X (2014) End-on covalent antibody immobilization on dual polarization interferometry sensor chip for enhanced immuno-sensing. Plasmonics 9(4):851–858
Song HY, Wong TI, Sadovoy A, Wu L, Bai P, Deng J, Guo S, Wang Y, Knoll W, Zhou X (2015a) Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots. Lab Chip 15(1):253–263
Song HY, Wong TI, Guo S, Deng J, Tan C, Gorelik S, Zhou X (2015b) Nanoimprinted thrombin aptasensor with picomolar sensitivity based on plasmon excited quantum dots. Sens Actuators B 221:207–216
Sun LL, Ng W, Zhou X, Leo YS, Wong TI (2016) Plasmonic biosensing based microfluidics point-of-care system for procalcitonin detection. Singapore provisional patent application 10201606001Q
Wong TI, Han S, Wu L, Wang Y, Deng J, Tan CYL, Bai P, Loke YC, Yang XD, Tse MS, Ng SH, Zhou X (2013) High throughput and high yield nanofabrication of precisely designed gold nanohole array for fluorescence enhanced detection on biomarkers. Lab Chip 13(12):2405–2413
Wu L, Bai P, Li EP (2012a) Designing surface plasmon resonance of subwavelength hole arrays by studying absorptance. J Opt Soc Am B 29(4):521–528
Wu L, Bai P, Zhou X, Li EP (2012b) Reflection and transmission modes in nanohole-array-based plasmonic sensors. IEEE Photonics J 4(1):26–33
Acknowledgments
The authors would like to express their gratitude to the A*STAR, Singapore, for funding the project 102 152 0014, and Ministry of Education, Singapore, for the project MOE2013-TIF-1-G-024.
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Zhou, X., Wong, T.I., Sun, L.L., Deng, J. (2017). Point-of-Care Device with Plasmonic Gold Nanoarray Sensing Chip for Biomarker Detections. In: Chandra, P., Tan, Y., Singh, S. (eds) Next Generation Point-of-care Biomedical Sensors Technologies for Cancer Diagnosis. Springer, Singapore. https://doi.org/10.1007/978-981-10-4726-8_14
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DOI: https://doi.org/10.1007/978-981-10-4726-8_14
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