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Flow Cytometry in Microbiology: The Reason and the Need

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Single Cell Analysis

Part of the book series: Series in BioEngineering ((SERBIOENG))

Abstract

The diagnosis of infection is based on methodologies that are, even in the 21st century, based on the study of the ability of microorganisms to grow in the presence of different substrates in the case of identification or in the presence of different antimicrobial drugs in the case of susceptibility evaluation. Despite the use of revolutionary techniques like molecular biology and more recently mass spectrometry, a lot of effort needs to be made to speed the results and to understand what happens to cells as individuals and not just as populations. Flow cytometry is an excellent tool still unexplored in microbiology; several applications are here described in the hope of contributing to the real use of flow cytometry in the clinical lab and to inspire future applications.

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Porto and Faculty of Medicine, Al. Hernâni Monteiro, 4200-319, Porto, Portugal

    Cidália Pina-Vaz, Sofia Costa-de-Oliveira, Ana Silva-Dias, Ana Pinto Silva, Rita Teixeira-Santos & Acácio Gonçalves Rodrigues

Authors
  1. Cidália Pina-Vaz
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  2. Sofia Costa-de-Oliveira
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  3. Ana Silva-Dias
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  4. Ana Pinto Silva
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  5. Rita Teixeira-Santos
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  6. Acácio Gonçalves Rodrigues
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Corresponding author

Correspondence to Cidália Pina-Vaz .

Editor information

Editors and Affiliations

  1. Purdue University Cytometry Laboratories, Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA

    J. Paul Robinson

  2. Chair of Pathology and Immunology, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy

    Andrea Cossarizza

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Pina-Vaz, C., Costa-de-Oliveira, S., Silva-Dias, A., Silva, A.P., Teixeira-Santos, R., Rodrigues, A.G. (2017). Flow Cytometry in Microbiology: The Reason and the Need. In: Robinson, J., Cossarizza, A. (eds) Single Cell Analysis. Series in BioEngineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-4499-1_7

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  • DOI: https://doi.org/10.1007/978-981-10-4499-1_7

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-4498-4

  • Online ISBN: 978-981-10-4499-1

  • eBook Packages: EngineeringEngineering (R0)

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