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Cancer Stem Cells and Multi-drug Resistance by Flow Cytometry

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Single Cell Analysis

Part of the book series: Series in BioEngineering ((SERBIOENG))

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Abstract

This chapter will focus on the study of stem cells, reviewing and updating a series of methods and protocols mainly applied in the author’s laboratory to learn more from the hematopoietic system, one of the best-studied compartments so far. The lack of specific markers for stem cells makes the physical identification and isolation of this compartment difficult; the author will introduce different methodologies aimed to identify as well as to isolate very rare stem cells and their subpopulations using functional flow cytometry, which has advantages over many other methods for identifying very primitive precursor cells. With all this information, researchers interested in the identification of cancer or leukemic stem cells should take into account that flow cytometric–based experiments must be carefully evaluated and planned. If not, these elusive cells can be mismeasured, which results in frustrating and unsuccessful time-consuming efforts.

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Authors and Affiliations

  1. Josep Carreras Leukaemia Research Institute, Badalona, Barcelona, Spain

    Jordi Petriz

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  1. Jordi Petriz
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Correspondence to Jordi Petriz .

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  1. Purdue University Cytometry Laboratories, Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA

    J. Paul Robinson

  2. Chair of Pathology and Immunology, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy

    Andrea Cossarizza

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Petriz, J. (2017). Cancer Stem Cells and Multi-drug Resistance by Flow Cytometry. In: Robinson, J., Cossarizza, A. (eds) Single Cell Analysis. Series in BioEngineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-4499-1_12

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  • DOI: https://doi.org/10.1007/978-981-10-4499-1_12

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