Abstract
The novel micro/nano cell and molecule biosensors are developed based on the traditional microfabrication and novel nanotechnology. Traditional biosensors, such as microelectrode array, impedance sensor, field-effect transistor, and light addressable potentiometric sensor, are useful tools in studying the cell biology and molecule analysis, while the nanobiosensor- and nanomaterial modified biosensors emerge gradually with the advance of nanotechnology. These nanobiosensor can achieve the single cell monitoring with high-quality signals, and nanomaterial modified biosensors have demonstrated excellent performance in cell and molecule applications. Combination of sensor detection technology and nanotechnology, the novel micro/nano cell, and molecule biosensors can explore a wide way in fields of biomedicine and environment monitoring.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fanigliulo A, Accossato P, Adami M, Lanzi M, Martinoia S, Paddeu S, Parodi M, Rossi A, Sartore M, Grattarola M. Comparison between a LAPS and an FET-based sensor for cell-metabolism detection. Sensors Actuators B Chem. 1996;32(1):41–8.
Hu N, Wu C, Ha D, Wang T, Liu Q, Wang P. A novel microphysiometer based on high sensitivity LAPS and microfluidic system for cellular metabolism study and rapid drug screening. Biosens Bioelectron. 2013;40(1):167–73.
Giaever I, Keese CR. A morphological biosensor for mammalian cells. Nature. 1993;366(6455):591–2.
Giaever I, Keese C. Monitoring fibroblast behavior in tissue culture with an applied electric field. Proc Natl Acad Sci. 1984;81(12):3761–4.
Giaever I, Keese CR. Micromotion of mammalian cells measured electrically. Proc Natl Acad Sci U S A. 1991;88(17):7896–900.
Keese CR, Wegener J, Walker SR, Giaever I. Electrical wound-healing assay for cells in vitro. Proc Natl Acad Sci U S A. 2004;101(6):1554–9.
Hess LH, Jansen M, Maybeck V, Hauf MV, Seifert M, Stutzmann M, Sharp ID, Offenhäusser A, Garrido JA. Graphene transistor arrays for recording action potentials from electrogenic cells. Adv Mater. 2011;23(43):5045–9.
Xiao L, Hu Z, Zhang W, Wu C, Yu H, Wang P. Evaluation of doxorubicin toxicity on cardiomyocytes using a dual functional extracellular biochip. Biosens Bioelectron. 2010;26(4):1493–9.
Brüggemann D, Wolfrum B, Maybeck V, Mourzina Y, Jansen M, Offenhäusser A. Nanostructured gold microelectrodes for extracellular recording from electrogenic cells. Nanotechnology. 2011;22(26):265104.
Xie C, Lin Z, Hanson L, Cui Y, Cui B. Intracellular recording of action potentials by nanopillar electroporation. Nat Nanotechnol. 2012;7(3):185–90.
Zeng D, Zhang H, Zhu D, Li J, San L, Wang Z, Wang C, Wang Y, Wang L, Zuo X, Mi X. A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures. Biosens Bioelectron. 2015;71:434–8.
Li J, Lee E-C. Carbon nanotube/polymer composite electrodes for flexible, attachable electrochemical DNA sensors. Biosens Bioelectron. 2015;71:414–9.
Wang H-B, Zhang H-D, Chen Y, Liu Y-M. A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA. Biosens Bioelectron. 2015;74:581–6.
Drexler KE. Engine of creation. The coming era of nanotechnology. New York: Anchor Books; 1986.
Drexler KE. Nanosystems: molecular machinery, manufacturing, and computation. New York: Wiley; 1992.
Sahoo S, Parveen S, Panda J. The present and future of nanotechnology in human health care. Nanomed Nanotechnol Biol Med. 2007;3(1):20–31.
Nel A, Xia T, Mädler L, Li N. Toxic potential of materials at the nanolevel. Science. 2006;311(5761):622–7.
Thomas C, Springer P, Loeb G, Berwald-Netter Y, Okun L. A miniature microelectrode array to monitor the bioelectric activity of cultured cells. Exp Cell Res. 1972;74(1):61–6.
Gesteland R, Howland B, Lettvin J, Pitts W. Comments on microelectrodes. Proc IRE. 1959;47(11):1856–62.
Robinson DA. The electrical properties of metal microelectrodes. Proc IEEE. 1968;56(6):1065–71.
Gross G, Rieske E, Kreutzberg G, Meyer A. A new fixed-array multi-microelectrode system designed for long-term monitoring of extracellular single unit neuronal activity in vitro. Neurosci Lett. 1977;6(2):101–5.
Gross GW. Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface. Biomed Eng IEEE Trans. 1979;5:273–9.
Pine J. Recording action potentials from cultured neurons with extracellular microcircuit electrodes. J Neurosci Methods. 1980;2(1):19–31.
Gross GW, Rhoades B, Jordan R. Neuronal networks for biochemical sensing. Sensors Actuators B Chem. 1992;6(1):1–8.
Borkholder D, DeBusschere BD, Kovacs G. An approach to the classification of unknown biological agents with cell based sensors. 1998.
DeBusschere BD, Kovacs GT. Portable cell-based biosensor system using integrated CMOS cell-cartridges. Biosens Bioelectron. 2001;16(7):543–56.
Gross GW, Rhoades BK, Azzazy HM, Wu M-C. The use of neuronal networks on multielectrode arrays as biosensors. Biosens Bioelectron. 1995;10(6):553–67.
Gross GW, Schwalm FU. A closed flow chamber for long-term multichannel recording and optical monitoring. J Neurosci Methods. 1994;52(1):73–85.
Rhoades BK, Gross GW. Potassium and calcium channel dependence of bursting in cultured neuronal networks. Brain Res. 1994;643(1):310–8.
Giaever I, Keese CR. Use of electric fields to monitor the dynamical aspect of cell behavior in tissue culture. Biomed Eng IEEE Trans. 1986;2:242–7.
Mitra P, Keese CR, Giaever I. Electric measurements can be used to monitor the attachment and spreading of cells in tissue culture. Biotechniques. 1991;11(4):504–10.
Keese CR, Giaever I. A whole cell biosensor based on cell-substrate interactions. In Editor (Ed.)^(Eds.): Book A whole cell biosensor based on cell-substrate interactions (IEEE, 1990, edn.), p. 500–501.
Keese CR, Giaever I. A biosensor that monitors cell morphology with electrical fields. Eng Med Biol Magaz IEEE. 1994;13(3):402–8.
Lo C-M, Keese CR, Giaever I. Monitoring motion of confluent cells in tissue culture. Exp Cell Res. 1993;204(1):102–9.
Lo C-M, Keese CR, Giaever I. pH changes in pulsed CO2 incubators cause periodic changes in cell morphology. Exp Cell Res. 1994;213(2):391–7.
Ghosh PM, Keese CR, Giaever I. Morphological response of mammalian cells to pulsed ac fields. Bioelectrochem Bioenerg. 1994;33(2):121–33.
Xiao C, Lachance B, Sunahara G, Luong JH. Assessment of cytotoxicity using electric cell-substrate impedance sensing: concentration and time response function approach. Anal Chem. 2002;74(22):5748–53.
Wang L, Zhu J, Deng C, Xing W-l, Cheng J. An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing. Lab Chip. 2008;8(6):872–8.
Ehret R, Baumann W, Brischwein M, Schwinde A, Stegbauer K, Wolf B. Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures. Biosens Bioelectron. 1997;12(1):29–41.
Chang B, Chen C, Ding S, Chen DC, Chang H. Impedimetric monitoring of cell attachment on interdigitated microelectrodes. Sensors Actuators B Chem. 2005;105(2):159–63.
Wang L, Wang H, Mitchelson K, Yu Z, Cheng J. Analysis of the sensitivity and frequency characteristics of coplanar electrical cell–substrate impedance sensors. Biosens Bioelectron. 2008;24(1):14–21.
Bergveld P. Development of an ion-sensitive solid-state device for neurophysiological measurements. IEEE Trans Biomed Eng. 1970;1:70–71c. BME-17.
Chang K-S, Sun C-J, Chiang P-L, Chou A-C, Lin M-C, Liang C, Hung H-H, Yeh Y-H, Chen C-D, Pan C-Y. Monitoring extracellular K+ flux with a valinomycin-coated silicon nanowire field-effect transistor. Biosens Bioelectron. 2012;31(1):137–43.
Fromherz P. Semiconductor chips with ion channels, nerve cells and brain. Phys E Low-Dimension Syst Nanostruct. 2003;16(1):24–34.
Ohtake T, Hamai C, Uno T, Tabata H, Kawai T. Immobilization of probe DNA on Ta2O5 thin film and detection of hybridized helix DNA using IS-FET. Jpn J Appl Phys. 2004;43(9A):L1137.
Ueno K, Inoue I, Akoh H, Kawasaki M, Tokura Y, Takagi H. Field-effect transistor on SrTiO3 with sputtered Al2O3 gate insulator, arXiv preprint cond-mat/0306436. 2003.
Finn A, Alderman J, Schweizer J. Towards an optimization of FET-based bio-sensors. Eur Cells Mater. 2002;4(Sup 2):21–3.
Oesch U, Caras S, Janata J. Field effect transistors sensitive to sodium and ammonium ions. Anal Chem. 1981;53(13):1983–6.
Bratov A, Abramova N, Domınguez C, Baldi A. Ion-selective field effect transistor (ISFET)-based calcium ion sensor with photocured polyurethane membrane suitable for ionised calcium determination in milk. Anal Chim Acta. 2000;408(1):57–64.
Zeck G, Fromherz P. Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip. Proc Natl Acad Sci. 2001;98(18):10457–62.
Fromherz P, Offenhausser A, Vetter T, Weis J. A neuron-silicon junction: a Retzius cell of the leech on an insulated-gate field-effect transistor. Science. 1991;252(5010):1290–3.
Hafeman DG, Parce JW, McConnell HM. Light-addressable potentiometric sensor for biochemical systems. Science. 1988;240(4856):1182–5.
Hafner F. Cytosensor® microphysiometer: technology and recent applications. Biosens Bioelectron. 2000;15(3):149–58.
Eklund SE, Snider RM, Wikswo J, Baudenbacher F, Prokop A, Cliffel DE. Multianalyte microphysiometry as a tool in metabolomics and systems biology. J Electroanal Chem. 2006;587(2):333–9.
Yicong W, Ping W, Xuesong Y, Gaoyan Z, Huiqi H, Weimin Y, Xiaoxiang Z, Jinghong H, Dafu C. Drug evaluations using a novel microphysiometer based on cell-based biosensors. Sensors Actuators B Chem. 2001;80(3):215–21.
Yicong W, Ping W, Xuesong Y, Qingtao Z, Rong L, Weimin Y, Xiaoxiang Z. A novel microphysiometer based on MLAPS for drugs screening. Biosens Bioelectron. 2001;16(4):277–86.
Hu N, Ha D, Wu C, Cheng G, Yu H, Wang T, Wu J, Cai H, Liu Q, Wang P. Design of microphysiometer based on multiparameter cell-based biosensors for quick drug analysis. J Innov Opt Health Sci. 2012;5(01):1150005.
Qing Q, Pal SK, Tian B, Duan X, Timko BP, Cohen-Karni T, Murthy VN, Lieber CM. Nanowire transistor arrays for mapping neural circuits in acute brain slices. Proc Natl Acad Sci. 2010;107(5):1882–7.
Cohen-Karni T, Qing Q, Li Q, Fang Y, Lieber CM. Graphene and nanowire transistors for cellular interfaces and electrical recording. Nano Lett. 2010;10(3):1098–102.
Giljohann DA, Mirkin CA. Drivers of biodiagnostic development. Nature. 2009;462(7272):461–4.
Scanziani M, Häusser M. Electrophysiology in the age of light. Nature. 2009;461(7266):930–9.
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth F. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981;391(2):85–100.
Tian B, Cohen-Karni T, Qing Q, Duan X, Xie P, Lieber CM. Three-dimensional, flexible nanoscale field-effect transistors as localized bioprobes. Science. 2010;329(5993):830–4.
Tian B, Xie P, Kempa TJ, Bell DC, Lieber CM. Single-crystalline kinked semiconductor nanowire superstructures. Nat Nanotechnol. 2009;4(12):824–9.
Patolsky F, Zheng G, Lieber CM. Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protoc. 2006;1(4):1711–24.
Duan X, Gao R, Xie P, Cohen-Karni T, Qing Q, Choe HS, Tian B, Jiang X, Lieber CM. Intracellular recordings of action potentials by an extracellular nanoscale field-effect transistor. Nat Nanotechnol. 2012;7(3):174–9.
Sakmann B. Single-channel recording. New York: Springer Science & Business Media; 2013.
Navarrete EG, Liang P, Lan F, Sanchez-Freire V, Simmons C, Gong T, Sharma A, Burridge PW, Patlolla B, Lee AS. Screening drug-induced arrhythmia using human induced pluripotent stem cell–derived cardiomyocytes and low-impedance microelectrode arrays. Circulation. 2013;128(11 suppl 1):S3–13.
Yamanaka K. Anodically electrodeposited iridium oxide films (AEIROF) from alkaline solutions for electrochromic display devices. Jpn J Appl Phys. 1989;28(4R):632.
Mafakheri E, Salimi A, Hallaj R, Ramazani A, Kashi MA. Synthesis of iridium oxide nanotubes by electrodeposition into polycarbonate template: fabrication of chromium (III) and arsenic (III) electrochemical sensor. Electroanalysis. 2011;23(10):2429–37.
Cogan SF, Ehrlich J, Plante TD, Smirnov A, Shire DB, Gingerich M, Rizzo JF. Sputtered iridium oxide films for neural stimulation electrodes. J Biomed Mater Res B Appl Biomater. 2009;89(2):353–61.
Lin ZC, Xie C, Osakada Y, Cui Y, Cui B. Iridium oxide nanotube electrodes for sensitive and prolonged intracellular measurement of action potentials. Nat Commun. 2014;5:1–10.
Zimmermann U, Pilwat G, Riemann F. Dielectric breakdown of cell membranes, Membrane transport in plants. Berlin: Springer; 1974. p. 146–53.
Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider P. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1982;1(7):841.
Chang D, Reese TS. Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy. Biophys J. 1990;58(1):1.
Zhai Y, Zhang Y, Qin F, Yao X. An electrochemical DNA biosensor for evaluating the effect of mix anion in cellular fluid on the antioxidant activity of CeO2 nanoparticles. Biosens Bioelectron. 2015;70:130–6.
Zhou J, Du L, Zou L, Zou Y, Hu N, Wang P. An ultrasensitive electrochemical immunosensor for carcinoembryonic antigen detection based on staphylococcal protein A—Au nanoparticle modified gold electrode. Sensors Actuators B Chem. 2014;197:220–7.
Aleshin AN. Polymer nanofibers and nanotubes: charge transport and device applications. Adv Mater. 2006;18(1):17–27.
Kaiser AB. Electronic transport properties of conducting polymers and carbon nanotubes. Rep Prog Phys. 2001;64(1):1.
Li J, Lu Y, Ye Q, Cinke M, Han J, Meyyappan M. Carbon nanotube sensors for gas and organic vapor detection. Nano Lett. 2003;3(7):929–33.
Yoon H, Lee SH, Kwon OS, Song HS, Oh EH, Park TH, Jang J. Polypyrrole nanotubes conjugated with human olfactory receptors: high-performance transducers for FET-type bioelectronic noses. Angew Chem Int Ed. 2009;48(15):2755–8.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Science Press and Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Hu, N., Fang, J., Zou, L. (2016). Micro/Nano Biosensors for Living Cell and Molecule Analysis. In: Wang, P., Wu, C., Hu, N., Hsia, K. (eds) Micro/Nano Cell and Molecular Sensors. Springer, Singapore. https://doi.org/10.1007/978-981-10-1658-5_2
Download citation
DOI: https://doi.org/10.1007/978-981-10-1658-5_2
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-1656-1
Online ISBN: 978-981-10-1658-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)