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Neuroprotection by Taurine on HBCD-Induced Apoptosis in PC12 Cells

  • Conference paper
Taurine 10

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 975))

Abstract

Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant (BFR). Because of their presence in human issues, including brain tissue, concern has been raised on their possible neurotoxicity. Presently, we explored the neuroprotection of taurine against HBCD-induced apoptotic damages in PC12 cells. Cells were pre-treated with taurine before HBCD exposure and the viability was assayed via the methyl-thiazolyl-tetrazolium (MTT) method. Apoptotic features were observed with Hoechst 33342 staining. Apoptotic ratio was measured using flow cytometry with Annexin V-FITC coupled propidium iodide (PI) double staining. The changes in the levels of Bcl-2 and Bax proteins were quantitated by the western blot. The activity of caspase-3 was tested and the results revealed that presence of HBCD decreased cell survival and led to apoptosis in the tested cells. Further, exposure of HBCD reduced protein expression of Bcl-2, increased expression in Bax protein and activity of caspase-3. Taurine attenuated HBCD-induced cell viability loss and cell apoptosis. Moreover, taurine significantly prevented from reducing Bcl-2 protein expression and elevating Bax protein expression and caspase-3 activity induced by HBCD. These results demonstrated that taurine can alleviate HBCD-induced apoptosis by altering Bcl-2 expression and Bax protein and Caspase-3 activity in PC12.

Shuangyue Li is co-first author.

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Abbreviations

HBCD:

Hexabromocyclododecane

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81102828, 81273037), the natural science foundation of Liaoning province of China (No. 201023054) and the natural science foundation of Shandong province of China (No. 2015ZRB14548).

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Authors and Affiliations

  1. School of Public Health, Dalian Medical University, No. 9 Western Section of Lushun South Road, Dalian, 116044, China

    Yachen Li, Shuangyue Li, Xiaohui Liu & Jing Shao

  2. College of Veterinary Medicine, Jilin University, Changchun, China

    Xizhe Xie

  3. The First Affiliated Hospital of Dalian Medical University, Dalian, China

    Hang Xiu

  4. School of Pharmaceutical Sciences, Binzhou Medical University, Yantai, 264003, China

    Xiuli Zhang

Authors
  1. Yachen Li
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  2. Shuangyue Li
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  3. Xizhe Xie
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  4. Hang Xiu
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  5. Xiaohui Liu
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  6. Jing Shao
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  7. Xiuli Zhang
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Corresponding authors

Correspondence to Yachen Li or Xiuli Zhang .

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Editors and Affiliations

  1. Department of Life Sciences, University of Seoul, Seoul, South Korea

    Dong-Hee Lee  & Ha Won Kim  & 

  2. College of Medicine, University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

  3. Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA

    Eunkyue Park

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Li, Y. et al. (2017). Neuroprotection by Taurine on HBCD-Induced Apoptosis in PC12 Cells. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_9

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