Abstract
As a new member of persistent organic pollutants, the potent neurotoxicity of perfluorooctane sulfonates (PFOS) found in epidemiological studies and laboratory research has drawn increasing attention around the world. Previous studies showed that apoptosis driven by oxidative stress and autophagy were both observed in PFOS-induced toxicity. Taurine has been demonstrated to exert potent protections against oxidative stress as an efficient antioxidant. Whether taurine could protect against the PFOS neurotoxicity is not known. In the present study, PC12 cells were treated with several concentrations of PFOS (31.25, 250 μM) for 24 h. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was applied to assess the cell viability. DCFH-DA detector was used to explore the production of ROS. Caspase 3 activity was used to reflect the possible apoptosis pathway. The lyso-tracker red dying was invited to evaluate the autophagy. Our data showed that taurine could significantly reverse the decreased viability and the increased ROS production in PC12 cells treated with PFOS. Moreover, the increased autophagy and apoptosis elicited by PFOS in PC12 cells could also be attenuated by taurine. Collectively, our results indicate that taurine may be an effective antioxidant in fighting against PFOS cytotoxicity and therefore could potentially serve as a preventative and therapeutic agent for environmental pollution-related toxicities.
Chunna Li and Xiaohui Liu contributed equally to this work.
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Abbreviations
- MTT:
-
4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide
- PFOS:
-
Perfluorooctane sulfonates
- ROS:
-
Reactive oxygen species
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (81273031/H2601 to J.S.; 81302400/H2601 to X.L.); and the startup funding from Dalian Medical University under the Talent Introduction Program (201069 to J.S.).
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Li, C. et al. (2017). Protection of Taurine Against PFOS-Induced Neurotoxicity in PC12 Cells. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_72
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