Abstract
Since the Arabidopsis genome was sequenced, hundreds of plant genomes have either been sequenced or are in sequencing progress. Reference genome sequences and large-scale genome sequencing technologies have initiated a new era in molecular breeding. The field of genomics is progressing rapidly and has already provided invaluable practical products for plant molecular breeding. Here, we review progress in genome sequencing technology and its application to plant breeding. We introduce various genomics tools and discuss how next-generation genome sequencing and genotyping technologies have been applied to high-throughput molecular breeding. We also describe the use of epigenome analysis to interpret phenotypic variations that cannot be explained by simple genetics based on the underlying DNA sequence alone, but rather by epigenetically-controlled mechanisms.
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References
Akimoto K, Katakami H, Kim HJ et al (2007) Epigenetic inheritance in rice plants. Ann Bot 100(2):205–217
Amoah S, Kurup S, Rodriguez Lopez CM et al (2012) A hypomethylated population of Brassica rapa for forward and reverse epi-genetics. BMC Plant Biol 12:193
Atwell S, Huang YS, Vilhjálmsson BJ et al (2010) Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines. Nature 465:627–631
Avery OT, Macleod CM, McCarty M (1944) Studies on the chemical nature of the substance inducing transformation of pneumococcal types: induction of transformation by a desoxyribonucleic acid fraction isolated from Pneumococcus type III. J Exp Med 79:137–158
Bailey-Serres J, Fukao T, Ronald P et al (2010) Submergence tolerant rice: SUB1’s journey from landrace to modern cultivar. Rice 3:138–147
Bashir A, Klammer AA, Robins WP et al (2012) A hybrid approach for the automated finishing of bacterial genomes. Nat Biotechnol 30:701–707
Bentley DR, Balasubramanian S, Swerdlow HP et al (2008) Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456:53–59
Bernstein BE, Meissner A, Lander ES (2007) The mammalian epigenome. Cell 128(4):669–681
Butler J, MacCallum I, Kleber M et al (2008) ALLPATHS: de novo assembly of whole-genome shotgun microreads. Genome Res 18:810–820
Chan M, Chan MW, Loh TW et al (2011) Evaluation of nanofluidics technology for high-throughput SNP genotyping in a clinical setting. J Mol Diagn 13(3):305–312
Chan SW, Henderson IR, Jacobsen SE (2005) Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat Rev Genet 6(5):351–360
Chen K, Gao C (2013) TALENs: customizable molecular DNA scissors for genome engineering of plants. J Genet Genomics 40(6):271–279
Choi SH, Lee BH, Kim HJ et al (2014) Ginseng gintonin activates the human cardiac delayed rectifier K+ channel: involvement of Ca2+/calmodulin binding sites. Mol Cells 37:656–663
CLC bio: CLC Assembly Cell user manual. http://www.clcbio.com/products/clc-genomics-workbench/
Cubas P, Vincent C, Coen E (1999) An epigenetic mutation responsible for natural variation in floral symmetry. Nature 401(6749):157–161
Eid J, Fehr A, Gray J et al (2009) Real-time DNA sequencing from single polymerase molecules. Science 323:133–138
English AC, Richards S, Han Y et al (2012) Mind the gap: upgrading genomes with pacific biosciences RS long-read sequencing technology. PLoS One 7(11):e47768
Gaj T, Gersbach CA, Barbas CF 3rd (2013) ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol 31(7):397–405
Hauben M, Haesendonckx B, Standaert E et al (2009) Energy use efficiency is characterized by an epigenetic component that can be directed through artificial selection to increase yield. Proc Natl Acad Sci U S A 106(47):20109–20114
Henderson IR, Jacobsen SE (2007) Epigenetic inheritance in plants. Nature 447(7143):418–424
Huang X, Feng Q, Qian Q et al (2009) High-throughput genotyping by whole-genome resequencing. Genome Res 19:1068–1076
International Human Genome Sequencing Consortium (2001) Initial sequencing and analysis of the human genome. Nature 409:860–921
International Rice Genome Sequencing Project (2005) The map-based sequence of the rice genome. Nature 436:793–800
Jacobsen SE, Meyerowitz EM (1997) Hypermethylated SUPERMAN epigenetic alleles in Arabidopsis. Science 277(5329):1100–1103
Jeddeloh JA, Stokes TL, Richards EJ (1999) Maintenance of genomic methylation requires a SW12/SNF2-like protein. Nat Genet 22(1):94–97
Johnson LM, Du J, Hale CJ et al (2014) SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation. Nature 507(7490):124–128
Kakutani T (1997) Genetic characterization of late-flowering traits induced by DNA hypomethylation mutation in Arabidopsis thaliana. Plant J 12(6):1447–1451
Kato M, Miura A, Bender J et al (2003) Role of CG and non-CG methylation in immobilization of transposons in arabidopsis. Curr Biol 13(5):421–426
Kim S, Park M, Yeom SI et al (2014) Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nat Genet 46(3):270–278
Koren S, Schatz MC, Walenz BP et al (2012) Hybrid error correction and de novo assembly of single-molecule sequencing reads. Nat Biotechnol 30:693–700
Lippman Z, Gendrel AV, Black M et al (2004) Role of transposable elements in heterochromatin and epigenetic control. Nature 430(6998):471–476
Lister R, O’Malley RC, Tonti-Filippini J et al (2008) Highly integrated single-base resolution maps of the epigenome in Arabidopsis. Cell 133(3):523–536
Liu WY, Kang JH, Jeong HS et al (2014a) Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper. Theor Appl Genet 127(11):2503–2513
Liu S, Liu Y, Yang X et al (2014b) The Brassica oleracea genome reveals the asymmetrical evolution of polyploidy genomes. Nat Commun 5:3930
Luo LJ (2010) Breeding for water-saving and drought-resistance rice (WDR) in China. J Exp Bot 61:3509–33517
Luo R, Liu B, Xie Y et al (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Giga Sci 1:18
Maeder ML, Angstman JF, Richardson ME et al (2013) Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol 31(12):1137–1142
Manning K, Tor M, Poole M et al (2006) A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nat Genet 38(8):948–952
Martin A, Troadec C, Boualem A et al (2009) A transposon-induced epigenetic change leads to sex determination in melon. Nature 461(7267):1135–1138
Mendenhall EM, Williamson KE, Reyon D et al (2013) Locus-specific editing of histone modifications at endogenous enhancers. Nat Biotechnol 31(12):1133–1136
Miura K, Agetsuma M, Kitano H et al (2009) A metastable DWARF1 epigenetic mutant affecting plant stature in rice. Proc Natl Acad Sci U S A 106(27):11218–11223
Miura K, Ikeda M, Matsubara A et al (2010) OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat Genet 42(6):545–549
Myers EW, Sutton GG, Delcher AL et al (2000) A whole-genome assembly of Drosophila. Science 287(5461):2196–2204
Palermo RE, Tisonici-Go J, Korth MJ et al (2013) Old world monkeys and new age science: the evolution of nonhuman primate systems virology. ILAR J 54(2):166–180
Pasam RK, Sharma R, Malosetti M et al (2012) Genome-wide association studies for agronomical traits in a worldwide spring barley collection. BMC Plant Biol 12:16
Poland JA, Brown PJ, Sorrells ME et al (2012) Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach. PLoS One 7(2):e32253. doi:10.1371/journal.pone.0032253
Quadrana L, Almeida J, Asis R et al (2014) Natural occurring epialleles determine vitamin E accumulation in tomato fruits. Nat Commun 5:3027
Quail MA, Smith M, Coupland P et al (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 13:341
Reinders J, Wulff BB, Mirouze M et al (2009) Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes. Genes Dev 23(8):939–950
Ribeiro FJ, Przybylski D, Yin S et al (2012) Finished bacterial genomes from shotgun sequence data. Genome Res 22:2270–2277
Rothberg JM, Leamon JH (2008) The development and impact of 454 sequencing. Nat Biotechnol 26(10):1117–1124
Saze H, Kakutani T (2007) Heritable epigenetic mutation of a transposon-flanked Arabidopsis gene due to lack of the chromatin-remodeling factor DDM1. EMBO J 26(15):3641–3652
Shendure J (2013) 2012 Curt stern award address. Am J Hum Genet 92(3):340–344
Sim S-C, Durstewitz G, Plieske J et al (2012) Development of a large SNP genotyping array and generation of high-density genetic maps in tomato. PLoS One 7:e40563
Song Q, Hyten DL, Jia G et al (2013) Development and evaluation of SoySNP50K, a high-density genotyping array for soybean. PLoS One 8(1):e54985
Soppe WJJ, Jacobsen SE, Alonso-Blanco C et al (2000) The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene. Mol Cell 6(4):791–802
The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Trebbi D, Maccaferri M, de Heer P et al (2011) High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.). Theor Appl Genet 123(4):555–569
Vongs A, Kakutani T, Martienssen RA et al (1993) Arabidopsis-thaliana DNA methylation mutants. Science 260(5116):1926–1928
Wang X, Wang H, Wang J et al (2011) The genome of the mesopolyploid crop species Brassica rapa. Nat Genet 43:1035–1039
Westengen OT, Berg PR, Kent MP et al (2012) Spatial structure and climatic adaptation in African maize revealed by surveying SNP diversity in relation to global breeding and landrace panels. PLoS One 7(10):e47832
Yang TJ, Kim JS, Kwon SJ (2006) Sequence-level analysis of the diploidization process in the triplicated FLOWERING LOCUS C region of Brassica rapa. Plant Cell 18:1339–1347
Zhang CQ, Hsieh T-F (2013) Heritable epigenetic variation and its potential applications for crop improvement. Plant Breed Biotechnol 1:307–319
Zhang K, Davenport KW, Gu W et al (2012) Improving genome assemblies by sequencing PCR products with PacBio. Biotechniques 53:61–62
Zhang X, Henderson IR, Lu C et al (2007) Role of RNA polymerase IV in plant small RNA metabolism. Proc Natl Acad Sci U S A 104:4536–4541
Zhang X, Yazaki J, Sundaresan A et al (2006) Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis. Cell 126(6):1189–1201
Zhao K, Tung CW, Eizenga GC et al (2011) Genome-wide association mapping reveals a rich genetic architecture of complex traits in Oryza sativa. Nat Commun 2:467
Zhong S, Fei Z, Chen YR et al (2013) Single-base resolution methylomes of tomato fruit development reveal epigenome modifications associated with ripening. Nat Biotechnol 31(2):154–159
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Choi, IY., Joh, HJ., Yi, G., Huh, J.H., Yang, TJ. (2015). Genomics-Assisted Breeding. In: Koh, HJ., Kwon, SY., Thomson, M. (eds) Current Technologies in Plant Molecular Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9996-6_5
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DOI: https://doi.org/10.1007/978-94-017-9996-6_5
Publisher Name: Springer, Dordrecht
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