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Strategies for Enhanced Production of Plant Secondary Metabolites from Cell and Organ Cultures

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Production of Biomass and Bioactive Compounds Using Bioreactor Technology

Abstract

Plant cell and organ cultures have emerged as a potential source of secondary metabolites which are used as pharmaceuticals, agrochemicals, flavours, fragrances, colouring agents, pesticides and food additives. Various strategies have been developed over past decades for biomass accumulation and synthesis of valuable compounds. Biosynthesis of secondary metabolites are generally not directly associated with cell growth. For the enhanced production of secondary metabolites, selection of high-yielding cell or organ clones, optimization of medium and physical factors which regulate the growth and accumulation of biomass are usually done at first, then in the secondary metabolite production stage, various strategies such as elicitation, precursor feeding, replenishment of nutrients are conducted. Permeabilization and immobilization are also proved to be important for the biosynthesis of secondary metabolites in some cases. By these strategies, it is possible to produce enormous biomass with improved accumulation of secondary metabolites.

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Abbreviations

2, 4-D:

2, 4-dichlorophenoxy acetic acid

2-iP:

2-isopentenyladenine

ABA:

Abscisic acid

B5:

Gamborg’s medium

BA:

Benzyladenine

DMSO:

Dimethylsulfoxide

DW:

Dry weight

FW:

Fresh weight

GA:

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

LS:

Linsmaier and Skoog medium

MS:

Murashige and Skoog medium

PUFAs:

Polyunsaturated fatty acids

SH:

Schenk and Hildebrandt medium

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Acknowledgement

This study was supported by a grant of Korea Healthcare Technology R&D project, Ministry of Health and Welfare, Republic of Korea (Grant No. A103017). Dr. H. N. Murthy is thankful to Ministry of Education, Science and Technology, Republic of Korea for the award of Brain pool Fellowship (131S-4-3-0523) and this paper was studied with the support of Ministry of Science, ICT and Planning (MSIP). JJZ acknowledges the support from National Natural Science Foundation of China (No. 21176153) and the Program of Shanghai Subject Chief Scientist (No. 14XD1402600).

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 361-763, Cheongju, Republic of Korea

    Hosakatte Niranjana Murthy & Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, 580003, Dharwad, India

    Hosakatte Niranjana Murthy & Vijayalaxmi S. Dandin

  3. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, 200240, Shanghai, China

    Jian-Jiang Zhong

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  1. Hosakatte Niranjana Murthy
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  2. Vijayalaxmi S. Dandin
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  3. Jian-Jiang Zhong
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  4. Kee-Yoeup Paek
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Correspondence to Hosakatte Niranjana Murthy .

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  1. Department of Horticultural Science, Chungbuk National University, Cheongju, Korea, Republic of (South Korea)

    Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, India

    Hosakatte Niranjana Murthy

  3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

    Jian-Jiang Zhong

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Murthy, H.N., Dandin, V.S., Zhong, JJ., Paek, KY. (2014). Strategies for Enhanced Production of Plant Secondary Metabolites from Cell and Organ Cultures. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_20

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