Abstract
We have previously shown that grape cells (Vitis vinifera L. cv Gamay) grown in vitro generate ethylene mostly from intracellular ACC [1]. In contrast ripening tomato fruits synthesize ethylene mainly from the apoplasm [2]. In the present work, we have transformed Gamay grape cells with the tomato pTOM 13 cDNA encoding tomato ACC oxidase and corresponding to a mRNA highly expressed during ripening 13]. The aim of this study is to determine where the pTOM 13-encoded protein is targeted in transgenic grape cells.
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Ayub, R.A., Rombaldi, C., Petiprez, M., Latché, A., Pech, J.C., Lelièvre, J.M. (1993). Biochemical and Immunocytological Characterization of ACC Oxidase in Transgenic Grape Cells. In: Pech, J.C., Latché, A., Balagué, C. (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene. Current Plant Science and Biotechnology in Agriculture, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1003-9_20
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DOI: https://doi.org/10.1007/978-94-017-1003-9_20
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