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Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis

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Proteins and Proteomics of Leishmania and Trypanosoma

Part of the book series: Subcellular Biochemistry ((SCBI,volume 74))

Abstract

Leishmania spp. are digenetic parasites which cause a broad spectrum of fatal diseases in humans. These parasites, as well as the other trypanosomatid, regulate gene expression at the post-transcriptional and post-translational levels, so that a poor correlation is observed between mRNA content and translated proteins. The completion of the genomic sequencing of several Leishmania species has enormous relevance to the study of the leishmaniasis pathogenesis. The combination of the available genomic resources of these parasites with powerful high-throughput proteomic analysis has shed light on various aspects of Leishmania biology as well as on the mechanisms underlying the disease. Diverse proteomic approaches have been used to describe and catalogue global protein profiles of Leishmania spp., reveal changes in protein expression during development, determine the subcellular localization of gene products, evaluate host-parasite interactions and elucidate drug resistance mechanisms. The characterization of these proteins has advanced, although many fundamental questions remain unanswered. Here, we present a historic review summarizing the different proteomic technologies applied to the study of Leishmania parasites during the last decades and we discuss the proteomic discoveries that have contributed to the understanding of Leishmania parasites biology and leishmaniasis.

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Abbreviations

1DE:

One dimensional electrophoresis

2D-DIGE:

Two dimensional differential gel electrophoresis

2DE:

Two dimensional electrophoresis

2D-LC-MS/MS:

Two dimensional liquid chromatography coupled with tandem mass spectrometry

ATP:

Adenosine triphosphate

BLAST:

Basic local alignment search tool

DNA:

Deoxyribonucleic acid

EDTA:

Ethylenediamine tetra acetic acid

ESI:

Electrospray ionization

ESI-MS:

Electrospray ionization mass spectrometry

GFP:

Green fluorescent protein

GO:

Gene ontology

GP46:

Glycoprotein 46

GP63:

Glycoprotein 63 also called leishmanolysin and major surface peptidase

GTP:

Guanosine triphosphate

HSP60:

Heat shock protein 60

HSP70:

Heat shock protein 70

HSP90:

Heat shock protein 90

HSP100:

Heat shock protein 100

IEF:

Isoelectric focalization

IgG:

Immunoglobulin g

IgM:

Immunoglobulin m

iMAC:

Metal affinity chromatography

iTRAQ:

Isobaric tags for relative and absolute quantification

KMP-11:

Kinetoplastid membrane protein 11

LC-MS/MS:

Liquid chromatography coupled with tandem mass spectrometers

MALDI:

Matrix assisted laser desorption ionization

MALDI-MS:

Matrix assisted laser desorption ionization mass spectrometry

MALDI-MS/MS:

Matrix assisted laser desorption ionization tandem mass spectrometry

mRNA:

Messenger ribonucleic acid

MUDPIT:

Multidimensional protein identification

PTMs:

Post translational modifications

Rab7:

Ras-related protein 7

RNA:

Ribonucleic acid

SbIII:

Trivalent antimonials

SbV:

Pentavalent antimonials

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SHERP:

Small hydrophilic endoplasmic reticulum-associated protein

SILAC:

Stable isotope labeling by amino acids in cell culture

SKRP:

Small-kinetoplastid-related-protein

TCA:

Trichloroacetic acid

Th1:

T helper 1

WHO:

World health organization

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Authors and Affiliations

  1. Departamento de Engenharia de Biossistemas, Universidade Federal de São João Del Rei, São João Del Rei, MG, Brazil

    Jose Batista de Jesus

  2. Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil

    Jose Batista de Jesus & Camila Mesquita-Rodrigues

  3. Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil

    Patricia Cuervo

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  1. Jose Batista de Jesus
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  2. Camila Mesquita-Rodrigues
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  3. Patricia Cuervo
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Correspondence to Jose Batista de Jesus .

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Editors and Affiliations

  1. Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    André L.S. Santos

  2. Departamento de Microbiologia Geral, Ins. de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    Marta H. Branquinha

  3. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Claudia M. d’Avila-Levy

  4. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Lucimar F. Kneipp

  5. Departamento de Biologia Celular e Molecular, Instituto de Biologia Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Cátia L. Sodré

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de Jesus, J.B., Mesquita-Rodrigues, C., Cuervo, P. (2014). Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis. In: Santos, A., Branquinha, M., d’Avila-Levy, C., Kneipp, L., Sodré, C. (eds) Proteins and Proteomics of Leishmania and Trypanosoma. Subcellular Biochemistry, vol 74. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7305-9_14

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