Abstract
This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts – K3[Fe(СN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.
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Acknowledgements
The work was supported by the Grant of RF Ministry “bioluminescent biotechnologies” N 11.G34.31.
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© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht
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Tarasova, A.S., Kudryasheva, N.S. (2013). Reaction Rates in Enzymatic Assay System in Solutions of Metal Salts and Humic Substances. In: Xu, J., Wu, J., He, Y. (eds) Functions of Natural Organic Matter in Changing Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5634-2_101
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DOI: https://doi.org/10.1007/978-94-007-5634-2_101
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