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The Role of Chondrogenic Factors in Differentiation of Bone Marrow Stromal Cells to the Cartilage Lineage

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Stem Cells and Cancer Stem Cells, Volume 7

Part of the book series: Stem Cells and Cancer Stem Cells ((STEM,volume 7))

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Abstract

Bone marrow stromal cells (BMSCs) are an established cell choice for cartilage repair because they are easily harvested, expanded, and differentiated into a cartilage phenotype characterized by aggrecan and type II collagen production. Transforming growth factor β (TGF-β), insulin-like growth factor 1 (IGF-1), and Dexamethasone (Dex) all influence the process of chondrogenesis. Although difficult challenges remain for optimizing the use of BMSCs for cartilage tissue engineering, in vitro culture systems present an excellent opportunity for studying chondrogenesis and understanding how progenitor cells respond to their biological, chemical, and mechanical microenvironment.

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References

  • Barry F, Boynton RE, Liu B, Murphy JM (2001) Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components. Exp Cell Res 268:189–200

    Article  CAS  PubMed  Google Scholar 

  • Byers BA, Mauck RL, Chiang IE, Tuan RS (2008) Transient exposure to transforming growth factor beta 3 under serum-free conditions enhances the biomechanical and biochemical maturation of tissue-engineered cartilage. Tissue Eng Part A 14:1821–1834

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chen FH, Tuan RS (2008) Mesenchymal stem cells in arthritic diseases. Arthritis Res Ther 10:223

    Article  PubMed Central  PubMed  Google Scholar 

  • Derfoul A, Perkins GL, Hall DJ, Tuan RS (2006) Glucocorticoids promote chondrogenic differentiation of adult human mesenchymal stem cells by enhancing expression of cartilage extracellular matrix genes. Stem Cells 24:1487–1495

    Article  CAS  PubMed  Google Scholar 

  • Dickhut A, Pelttari K, Janicki P, Wagner W, Eckstein V, Egermann M, Richter W (2009) Calcification or dedifferentiation: requirement to lock mesenchymal stem cells in a desired differentiation stage. J Cell Physiol 219:219–226

    Article  CAS  PubMed  Google Scholar 

  • Fan H, Hu Y, Qin L, Li X, Wu H, Lv R (2006) Porous gelatin-chondroitin-hyaluronate tri-copolymer scaffold containing microspheres loaded with TGF-β1 induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair. J Biomed Mater Res A 77:785–794

    Article  PubMed  Google Scholar 

  • Fan J, Varshney RR, Ren L, Cai D, Wang DA (2009) Synovium-derived mesenchymal stem cells: a new cell source for musculoskeletal regeneration. Tissue Eng Part B Rev 15:75–86

    Article  CAS  PubMed  Google Scholar 

  • Florine EM, Vanderploeg EJ, Kopesky PW, Miller RE, Grodzinsky AJ (2010) Dexamethasone suppresses aggrecan catabolism in BMSC-seeded peptide hydrogels. In: 56th Transactions of the Orthopaedic Research Society, New Orleans 1326

    Google Scholar 

  • Getgood A, Brooks R, Fortier L, Rushton N (2009) Articular cartilage tissue engineering: today’s research, tomorrow’s practice? J Bone Joint Surg Br 91:565–576

    Article  CAS  PubMed  Google Scholar 

  • Goldring MB, Tsuchimochi K, Ijiri K (2006) The control of chondrogenesis. J Cell Biochem 97:33–44

    Article  CAS  PubMed  Google Scholar 

  • Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU (1998) In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res 238:265–272

    Article  CAS  PubMed  Google Scholar 

  • Kisiday JD, Kopesky PW, Evans CH, Grodzinsky AJ, McIlwraith CW, Frisbie DD (2008) Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures. J Orthop Res 26:322–331

    Article  CAS  PubMed  Google Scholar 

  • Kopesky PW, Vanderploeg EJ, Sandy JS, Kurz B, Grodzinsky AJ (2010) Self-assembling peptide hydrogels modulate in vitro chondrogenesis of bovine bone marrow stromal cells. Tissue Eng Part A 16:465–477

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kopesky PW, Vanderploeg EJ, Kisiday JD, Frisbie DD, Sandy JD, Grodzinsky AJ (2011) Controlled delivery of transforming growth factor β1 by self-assembling peptide hydrogels induces chondrogenesis of bone marrow stromal cells and modulates Smad2/3 signaling. Tissue Eng Part A 17:83–92

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kotlarz H, Gunnarsson CL, Fang H, Rizzo JA (2009) Insurer and out-of-pocket costs of osteoarthritis in the US: evidence from national survey data. Arthritis Rheum 60:3546–3553

    Article  PubMed  Google Scholar 

  • Martel-Pelletier J, Di Battista JA, Lajeunesse D, Pelletier JP (1998) IGF/IGFBP axis in cartilage and bone in osteoarthritis pathogenesis. Inflamm Res 47:90–100

    Article  CAS  PubMed  Google Scholar 

  • Mi Z, Ghivizzani SC, Lechman E, Glorioso JC, Evans CH, Robbins PD (2003) Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees. Arthritis Res Ther 5:R132–R139

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Miller RE, Grodzinsky AJ, Vanderploeg EJ, Lee C, Ferris DJ, Barrett MF, Kisiday JD, Frisbie DD (2010a) Effect of self-assembling peptide, chondrogenic factors, and bone marrow-derived stromal cells on osteochondral repair. Osteoarthritis Cartilage 18:1608–1619

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Miller RE, Grodzinsky AJ, Cummings K, Plaas AH, Cole AA, Lee RT, Patwari P (2010b) Intraarticular injection of heparin-binding insulin-like growth factor 1 sustains delivery of insulin-like growth factor 1 to cartilage through binding to chondroitin sulfate. Arthritis Rheum 62:3686–3694

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mouw JK, Connelly JT, Wilson CG, Michael KE, Levenston ME (2007) Dynamic compression regulates the expression and synthesis of chondrocyte-specific matrix molecules in bone marrow stromal cells. Stem Cells 25:655–663

    CAS  PubMed  Google Scholar 

  • Newton R, Holden NS (2007) Separating transrepression and transactivation: a distressing divorce for the glucocorticoid receptor? Mol Pharmacol 72:799–809

    Article  CAS  PubMed  Google Scholar 

  • Nixon AJ, Fortier LA, Williams J, Mohammed H (1999) Enhanced repair of extensive articular defects by insulin-like growth factor-I-laden fibrin composites. J Orthop Res 17:475–487

    Article  CAS  PubMed  Google Scholar 

  • Noth U, Steinert AF, Tuan RS (2008) Technology insight: adult mesenchymal stem cells for osteoarthritis therapy. Nat Clin Pract Rheumatol 4:371–380

    PubMed  Google Scholar 

  • Palmer GD, Steinert A, Pascher A, Gouze E, Gouze JN, Betz O, Johnstone B, Evans CH, Ghivizzani SC (2005) Gene-induced chondrogenesis of primary mesenchymal stem cells in vitro. Mol Ther 12:219–228

    Article  CAS  PubMed  Google Scholar 

  • Park JS, Na K, Woo DG, Yang HN, Park KH (2009) Determination of dual delivery for stem cell differentiation using dexamethasone and TGF-ß3 in/on polymeric microspheres. Biomaterials 30:4796–4805

    Article  CAS  PubMed  Google Scholar 

  • Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147

    Article  CAS  PubMed  Google Scholar 

  • Prockop DJ (2009) Repair of tissues by adult stem/progenitor cells (MSCs): controversies, myths, and changing paradigms. Mol Ther 17:939–946

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Schmidt MB, Chen EH, Lynch SE (2006) A review of the effects of insulin-like growth factor and platelet-derived growth factor on in vivo cartilage healing and repair. Osteoarthritis Cartilage 14:403–412

    Article  CAS  PubMed  Google Scholar 

  • Shah RN, Shah NA, Del Rosario Lim MM, Hsieh C, Nuber G, Stupp SI (2010) Supramolecular design of self-assembling nanofibers for cartilage regeneration. Proc Natl Acad Sci USA 107:3293–3298

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shi Y, Massague J (2003) Mechanisms of TGF-β signaling from cell membrane to the nucleus. Cell 113:685–700

    Article  CAS  PubMed  Google Scholar 

  • Starkman BG, Cravero JD, Delcarlo M, Loeser RF (2005) IGF-1 stimulation of proteoglycan synthesis by chondrocytes requires activation of the PI 3-kinase pathway but not ERK MAPK. Biochem J 389:723–729

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tokunou T, Miller R, Patwari P, Davis ME, Segers VF, Grodzinsky AJ, Lee RT (2008) Engineering insulin-like growth factor-1 for local delivery. FASEB J 22:1886–1893

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tuli R, Tuli S, Nandi S, Huang X, Manner PA, Hozack WJ, Danielson KG, Hall DJ, Tuan RS (2003) Transforming growth factor-β-mediated chondrogenesis of human mesenchymal progenitor cells involves N-cadherin and mitogen-activated protein kinase and Wnt signaling cross-talk. J Biol Chem 278:41227–41236

    Article  CAS  PubMed  Google Scholar 

  • van der Kraan PM, van den Berg WB (2000) Anabolic and destructive mediators in osteoarthritis. Curr Opin Clin Nutr Metab Care 3:205–211

    Article  PubMed  Google Scholar 

  • Worster AA, Brower-Toland BD, Fortier LA, Bent SJ, Williams J, Nixon AJ (2001) Chondrocytic differentiation of mesenchymal stem cells sequentially exposed to transforming growth factor-beta1 in monolayer and insulin-like growth factor-1 in a three-dimensional matrix. J Orthop Res 19:738–749

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Center for Biomedical Engineering, Massachusetts Institute of Technology, MIT Room NE47-377, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA

    Emily M. Florine & Alan J. Grodzinsky

Authors
  1. Emily M. Florine
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  2. Alan J. Grodzinsky
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Corresponding author

Correspondence to Alan J. Grodzinsky .

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Editors and Affiliations

  1. Biology, Kean University, Union, New Jersey, USA

    M.A. Hayat

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Florine, E.M., Grodzinsky, A.J. (2012). The Role of Chondrogenic Factors in Differentiation of Bone Marrow Stromal Cells to the Cartilage Lineage. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 7. Stem Cells and Cancer Stem Cells, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4285-7_15

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