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Biosensors for Metabolic Engineering

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Systems Biology Application in Synthetic Biology

Abstract

With the advent of synthetic biology, it is possible to accelerate metabolic engineering research due to lower cost gene synthesis. In particular, genetically encoded biosensors can be used in synthetic circuits to dynamically respond to metabolites to actuate desired metabolic engineering functions. Biosensors can be employed to target high-producing strains by high-throughput screening, to sense desirable products in selective conditions, and to dynamically control metabolic fluxes. In this chapter, we first describe different types of biosensors (transcription factor-based biosensors, RNA-based biosensors, protein activity-based biosensors, and whole cell biosensors) that have been developed. Next, we describe the application of developed biosensors well-developed methods that are used to develop novel biosensors. Finally, we provide some perspectives on biosensor utilization in metabolic engineering and some potential problems that face this field.

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Abbreviations

2-PS:

2-Pyrone synthase

acetyl-CoA:

Acetyl coenzyme A

ATF:

Artificial transcription factor

BLA:

β-Lactamase

bPBP:

Bacterial periplasmic binding protein

CIDs:

Chemical inducers of dimerization

DBDs:

DNA-binding domains

ER:

Estrogen receptor

eyfp:

Enhanced yellow fluorescence protein

FACS:

Fluorescence-activated cell sorting

FAEE:

Fatty acid ethyl ester

GC:

Gas chromatography

GFP:

Green fluorescent protein

HHRs:

Hammerhead ribozymes

HMG-CoA:

Hydroxymethylglutaryl-CoA

HPLC:

High-performance liquid chromatography

IPP:

Isopentenyl pyrophosphate

IPTG:

β-D-1-Thiogalactopyranoside

LBD:

Ligand-binding domain

MAGE:

Multiplex automated genome engineering

MBP:

Maltose-binding protein

MRTF:

Metabolite-responsive transcription factor

RBS:

Ribosome binding site

RD:

Regulatory domain

RFP:

Red fluorescent protein

SDS:

Sodium dodecyl sulfate

TAL:

Triacetic acid lactone

TATB:

1,3,5-Triamino-2,4,6-trinitrobenzene

TPP:

Thiamine pyrophosphate

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Authors and Affiliations

  1. Department of Chemical and Life Science Engineering, Virginia Commonwealth University, West Hall, Room 422, 601 West Main Street, 843028, Richmond, VA, 23284-3028, USA

    Qiang Yan & Stephen S. Fong

  2. Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, VA, USA

    Stephen S. Fong

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  1. Qiang Yan
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Correspondence to Stephen S. Fong .

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  1. Computational and Systems Biology Lab, National Centre for Cell Science, Pune, India

    Shailza Singh

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Yan, Q., Fong, S.S. (2016). Biosensors for Metabolic Engineering. In: Singh, S. (eds) Systems Biology Application in Synthetic Biology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2809-7_5

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