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Microbial Chassis Assisting Retrosynthesis

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Systems Biology Application in Synthetic Biology

Abstract

Synthetic biology has come a long way from constructing simple regulatory element to de novo pathway construction in heterologous host chassis. This is achieved by the transfer of the desired pathway from a rare organism to an organism that can be readily genetically engineered. These developments have great potential for application in biosynthesis of drugs, biofuels and bulk chemicals from simple and inexpensive starting material. As the complexity within a re-engineered system increases, there is an increasing need for efficient computational tools that can support them. Myriad of algorithms are available and are being developed that aid the re-engineering of pathways that help select and prioritize pathways, optimize enzyme performance, select parts for constructing the pathway, metabolic modelling and flux analysis and final integration into the chassis. This chapter gives a gist into the development of de novo pathway, the bioinformatics tools available, future challenges and research efforts needed for the implementation of synthetic biology for the production of key metabolites.

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Authors and Affiliations

  1. National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune, 411007, India

    Milsee Mol & Vineetha Mandlik

  2. Computational and Systems Biology Lab, National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune, 411007, India

    Shailza Singh

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    Shailza Singh

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Mol, M., Mandlik, V., Singh, S. (2016). Microbial Chassis Assisting Retrosynthesis. In: Singh, S. (eds) Systems Biology Application in Synthetic Biology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2809-7_1

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