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Nitrification Inhibitors: Classes and Its Use in Nitrification Management

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Nutrient Use Efficiency: from Basics to Advances

Abstract

The explosive expansion of human activity during the last two centuries through industrial and agricultural pursuits has resulted in massive changes in the nitrogen (N) cycle of the planet. Based on the projected population growth and food demand, the N-fertilizer inputs into agricultural systems need to be doubled in the near future which would lead to further increase in the amount of N lost to the environment. If production agriculture continues to move towards high-nitrification agricultural systems with the expansion and intensification of agricultural activities, there is potential for catastrophic consequences to our planet due to the destruction of the ozone layer, global warming, and eutrophication. It is therefore imperative to manage the nitrification in agricultural systems for minimizing N leaks into the environment which are not only a serious economic and energy drain on society but also potentially have long-term ecological and environmental consequences. Currently, more than 60 % of the total N applied to agricultural systems is lost, amounting to an annual economic loss equivalent to US$17 billion worldwide. Wide substrate range of ammonia monooxygenase (AMO), an important enzyme involved in nitrification, has enabled a range of chemicals or chemical formulations that can be effectively deployed as additives to N fertilizers to regulate nitrification. These chemicals by augmenting the efficiency of N-fertilizer use help us to achieve higher food production for catering the ever increasing population and minimize fertilizer-related pollution of the environment. This paper overviews N transformations in agricultural systems and the salient agrochemicals employed for management of nitrification, the most important transformation, in particular.

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References

  • Abalos D, Jeffery S, Sanz-Cobena A, Guardia G, Vallejo A (2014) Meta-analysis of the effect of urease and nitrification inhibitors on crop productivity and nitrogen use efficiency. Agric Ecosyst Environ 189:136–144

    Article  CAS  Google Scholar 

  • Abbasi MK, Adams WA (2000) Estimation of simultaneous nitrification and denitrification in grassland soil associated with urea-N and 15N and nitrification inhibitor. Biol Fertil Soils 31:38–44

    Article  CAS  Google Scholar 

  • Aggarwal N, Kumar R, Dureja P, Rawat DS (2009) Schiff bases as potential fungicides and nitrification inhibitors. J Agric Food Chem 57:8520–8525

    Article  CAS  PubMed  Google Scholar 

  • Aggarwal N, Kumar R, Srivastva C, Dureja P, Khurana JM (2010) Synthesis of nalidixic acid based hydrazones as novel pesticides. J Agric Food Chem 58(5):3056–3061

    Article  CAS  PubMed  Google Scholar 

  • Agricoop (2009) http://agricoop.nic.in/inm/cfqcti9210.pdf. Accessed on 13 Dec 2011

  • Azam F, Farooq S (2003) Nitrification inhibition in soil and ecosystem functioning – an overview. Pak J Biol Sci 6(6):528–535

    Article  Google Scholar 

  • Azam F, Mueller C, Weiske A, Benckiser G, Ottow JCG (2002) Nitrification and denitrification as sources of atmospheric N2O – role of oxidizable C and applied N. Biol Fertil Soils 35:54–61

    Article  CAS  Google Scholar 

  • Ball BC, Cameron KC, Di HJ, Moore S (2012) Effects of trampling of a wet dairy pasture soil on soil porosity and on mitigation of nitrous oxide emissions by a nitrification inhibitor, dicyandiamide. Soil Use Manag 28:194–201

    Article  Google Scholar 

  • Banerjee NK, Mosier AR, Uppal KS, Goswami NN (1990) Use of encapsulated calcium carbide to reduce denitrification losses from urea-fertilized flooded rice. Mitteilungen der Deutschen Bodenkundlichen Gesellschaft 60:245–248

    Google Scholar 

  • Bedard C, Knowles R (1989) Physiology, biochemistry, and specific Inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers. Microb Rev 53:68–84

    CAS  Google Scholar 

  • Bhatia A, Sasmal S, Jain N, Pathak H, Kumar R, Singh A (2010) Mitigating nitrous oxide emission from soil under conventional and no-tillage in wheat using nitrification inhibitors. Agric Econ Environ 136:247–253

    Article  CAS  Google Scholar 

  • Bothe H, Jost G, Schloter M, Ward BB, Witzel KP (2000) Molecular analysis of ammonia oxidation and denitrification in natural environments. FEMS Microbiol Rev 24:673–690

    Article  CAS  PubMed  Google Scholar 

  • Boudsocq S, Lata JC, Mathieu J, Abbadie L, Barot S (2009) Modelling approach to analyse the effects of nitrification inhibition on primary production. Funct Ecol 23:220–230

    Article  Google Scholar 

  • Boudsocq S, Barot S, Loeuille N (2011) Evolution of nutrient acquisition: When adaptation fills the gap between contrasting ecological theories. Proc R Soc B Biol Sci 278:449–457

    Article  CAS  Google Scholar 

  • Datta A, Walia S, Parmar BS (2001) Some furfural derivatives as nitrification inhibitors. J Agric Food Chem 49:4726–4731

    Article  CAS  PubMed  Google Scholar 

  • Duddleston KN, Bottomley PJ, Porter AJ, Arp DJ (2000) New insights into methyl bromide cooxidation by Nitrosomonas europaea obtained by experimenting with moderately low density cell suspensions. Appl Environ Microbiol 66:2726–2731

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Freney JR, Peoples MB, Mosier AR (2011) Efficient use of fertilizer nitrogen by crops. http://www.agnet.org/library/eb/414/. Accessed on 02 Dec 2011

  • Gopalakrishnan S, Subbarao GV, Nakahara K, Yoshihashi T, Ito O, Maeda I, Ono H, Yoshida M (2007) Nitrification inhibitors from the root tissues of Brachiaria humidicola, a tropical grass. J Agric Food Chem 55:1385–1388

    Article  CAS  PubMed  Google Scholar 

  • Hawkes CV, Wren IF, Herman DJ, Firestone MK (2005) Plant invasion alters nitrogen cycling by modifying the soil nitrifying community. Ecol Lett 8:976–985

    Article  Google Scholar 

  • Holmes AJ, Costello A, Lindstrom ME, Murrell JC (1995) Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionary related. FEMS Microbiol Lett 132:203–208

    Article  CAS  PubMed  Google Scholar 

  • Hovanec TA, Delong EF (1996) Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl Environ Microbiol 62(8):2888–2896

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jenkinson DS, Fox RH, Rayner JH (1985) Interactions between fertilizer nitrogen and soil nitrogen – the so-called “priming” effect. J Soil Sci 36:425–444

    Article  CAS  Google Scholar 

  • Kawakami EM, Oosterhuis DM, Snider JL, Mozaffari M (2012) Physiological and yield responses of field grown cotton to application of urea with urease inhibitor NBPT and the nitrification inhibitor DCD. Eur J Agron 43:147–154

    Article  CAS  Google Scholar 

  • Khan MG, Silberbush M, Lips SH (1994) Physiological studies on salinity and nitrogen interaction in alfalfa. I. Biomass production and root development. J Plant Nutr 17:657–668

    Article  Google Scholar 

  • Koops HP, Bottcher B, Moller UC, Pommerening-Roser A, Stehr G (1991) Classification of eight new species of ammonia-oxidizing bacteria: Nitrosomonas communis sp. nov., Nitrosomonas ureae sp. nov., Nitrosomonas aestuarii sp. nov., Nitrosomonas marina sp. nov., Nitrosomonas nitrosa sp. nov., Nitrosomonas eutropha sp. nov., Nitrosomonas oligotropha sp. nov. and Nitrosomonas halophila sp. nov. J Gen Microbiol 137:1689–1699

    Article  CAS  Google Scholar 

  • Kumar R, Anupama, Parmar BS (2004) S-Benzylisothiouronium derivatives as nitrification inhibitors. Pestic Res J 16(1):48–57

    CAS  Google Scholar 

  • Kumar R, Anupama, Parmar BS (2005) Process for the preparation of 5-substituted-1,3,4-oxadiazole-2-thiols as new urease and nitrification inhibitors. Patent application no. 3461/DEL/2005 filed 23 Dec 2005. Publication date: 05 Sept 2008

    Google Scholar 

  • Kumar R, Devakumar C, Sharma V, Kakkar G, Kumar D, Panneerselvam P (2007) Influence of physico-chemical parameters of neem (Azadirachta indica A Juss) oils on nitrification inhibition in soil. J Agric Food Chem 55:1389–1393

    Article  CAS  PubMed  Google Scholar 

  • Kumar R, Devakumar C, Kumar D, Panneerselvam P, Kakkar G, Arivalagan T (2008) Influence of edaphic factors on the mineralization of neem oil coated urea in four Indian soils. J Agric Food Chem 56(21):10183–10191

    Article  CAS  PubMed  Google Scholar 

  • Kumar D, Devakumar C, Kumar R, Das A, Panneerselvam P, Shivay YS (2010) Effect of neem-oil coated prilled urea with varying thickness of neem (Azadirachta indica A. Juss)-oil coating and N rates on productivity and nitrogen-use efficiency of lowland irrigated rice under Indo-Gangetic plains. J Plant Nutr 33:1939–1959

    Article  CAS  Google Scholar 

  • Kumar D, Devakumar C, Kumar R, Panneerselvam P, Das A, Shivay YS (2011) Relative efficiency of prilled urea coated with major neem (Azadirachta indica A. Juss) oil components in lowland irrigated rice of Indo-Gangetic plains. Arch Agron Soil Sci 57(1):61–74

    Article  CAS  Google Scholar 

  • Kuzvinzwa SM, Devakumar C, Mukherjee SK (1984) Evaluation of furano compounds as nitrification inhibitors. Bull Indian Soc Soil Sci 13:165–172

    Google Scholar 

  • Ma Y, Sun L, Zhang X, Yang B, Yin B, Yan X, Xiong Z (2013) Mitigation of nitrous oxide emissions from paddy soil under conventional and no-till practices using nitrification inhibitors during the winter wheat growing season. Biol Fertil Soils 49:627–635

    Article  CAS  Google Scholar 

  • Majumdar D (2008) Unexploited botanical nitrification inhibitors prepared from Karanja plant. Nat Prod Radiance 7(1):58–67

    Google Scholar 

  • Marschner H (1999) Mineral nutrition of higher plants, 2nd edn. Academic Press, London

    Google Scholar 

  • Mazumdar D (2002) Suppression of nitrification and N2O emission by Karanjin-a nitrification inhibitor prepared from Karanj (Pongamia glabra). Chemosphere 47:845–850

    Article  Google Scholar 

  • McCarty GW (1999) Modes of nitrification inhibitors. Biol Fertil Soils 29:1–9

    Article  CAS  Google Scholar 

  • McCarty GW, Bremner JM (1989) Inhibition of nitrification in soil by heterocyclic nitrogen compounds. Biol Fertil Soils 8:204–211

    CAS  Google Scholar 

  • Menendez S, Barrena I, Setien I, Gonzalez-Murua C, Estavillo MJ (2012) Efficiency of nitrification inhibitor DMPP to reduce nitrous oxide emissions under different temperature and moisture conditions. Soil Biol Biochem 53:82–89

    Article  CAS  Google Scholar 

  • Norton JM, Stark JM (2011) Regulation and measurement of nitrification in terrestrial systems. In: Klotz MG (ed) Methods in enzymology, vol 486. Academic Press, Burlington, pp 343–368

    Google Scholar 

  • Opoku A, Chaves B, De Neve S (2014) Neem seed oil: a potent nitrification inhibitor to control nitrate leaching after incorporation of crop residues. Biol Agric Hortic. doi:10.1080/01448765.2014.885394

    Google Scholar 

  • Patra DD, Chand S (2009) Natural nitrification inhibitors for augmenting nitrogen use efficiency in soil-plant system. In: The proceedings of the international plant nutrition colloquium XVI, Department of Plant Sciences, UC Davis, UC Davis. http://escholarship.org/uc/item/4h30z8tg. Accessed on 03 Dec 2011

  • Prasad R (1998) Fertilizer urea, food security, health and the environment. Curr Sci 75:677–683

    Google Scholar 

  • Prasad R, Power JF (1995) Nitrification inhibitors for agriculture, health and the environment. Adv Agron 54:233–281

    Article  CAS  Google Scholar 

  • Prasad R, Devakumar C, Shivay YS (1993) Significance in increasing fertilizer nitrogen efficiency. In: Randhawa NS, Parmar BS (ed) Neem research and development, SPS publication no. 3, Society of Pesticide Science, India, New Delhi, pp 97–108

    Google Scholar 

  • Prasad R, Sharma SN, Singh S, Devakumar C, Saxena VS (2002) Neem coating of urea for the environment and agriculture. Fertil News 47(5):63–67

    Google Scholar 

  • Purkhold U, Pommerening-Roser A, Juretschko S, Schmid MC, Koops HP, Wagner M (2000) Phylogeny of all recognized species of ammonia oxidizers based on comparative \6S rRNA and amoA sequence analysis: implications for molecular diversity studies. Appl Environ Microbiol 66:5368–5382

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Raun WR, Johnson GV (1999) Improving nitrogen use efficiency for cereal production. Agron J 91:357–363

    Article  Google Scholar 

  • Regan John M, Harrington GW, Noguera DR (2002) Ammonia- and nitrite-oxidizing bacterial communities in a pilot-scale chloraminated drinking water distribution system. Appl Environ Microbiol 68(1):73–81

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rice EL (1984) Allelopathy, 2nd edn. Academic Press, New York

    Google Scholar 

  • Rossiter-Rachor NA, Setterfield SA, Douglas MM, Hutley LB, Cook GD, Schmidt S (2009) Invasive Andropogon gayanus (Gamba Grass) is an ecosystem transformer of nitrogen reactions in Australian savanna. Ecol Appl 19:1546–1560

    Article  CAS  PubMed  Google Scholar 

  • Saha A, Kumar R, Kumar R, Devakumar C (2010) Green synthesis of 5-substituted-1,3,4-thiadiazole-2-thiols as new potent nitrification inhibitors. J Heteroc Chem 47:838–845

    Article  CAS  Google Scholar 

  • Sahrawat KL (1982) Comparative evaluation of Karanjin and extract of Karanj (Pongamia glabra Vent) and neem (Azadirachta indica) seeds for retardation of nitrification of urea in soil. J Indian Soc Soil Sci 30:156–159

    Google Scholar 

  • Sahrawat KL, Mukherjee SK (1977) Nitrification inhibitors: I. Studies with furano compounds. Plant Soil 47:687–691

    Article  CAS  Google Scholar 

  • Sahrawat KL, Parmar BS (1975) Alcohol extract of neem (Azadirachta indica) as nitrification inhibitor. J Indian Soc Soil Sci 13:131–134

    Google Scholar 

  • Sahrawat KL, Parmar BS, Mukherjee SK (1974) A note on the nitrification inhibitors in the seeds, bark and leaves of Pongamia glabra Vent. Indian J Agric Sci 44:415–418

    CAS  Google Scholar 

  • Sanz-Cobena A, Sanchez-Martin L, Garcia-Torres L, Vallejo A (2012) Gaseous emission of N2O and NO and NO3 leaching from urea applied with urease and nitrification inhibitors to a maize crop. Agric Ecosyst Environ 149:64–73

    Article  CAS  Google Scholar 

  • Saxena VS, Devakumar C, Prasad R (1999) Pusaneem-ME coated urea. Indian patent application no. 223/Del/99 dated 5th Feb 1999

    Google Scholar 

  • Shaviv A, Mikkelsen RL (1993) Controlled release fertilizers to increase efficiency of nutrient use and minimize environmental degradation: a review. Fertil Res 35:1–12

    Article  CAS  Google Scholar 

  • Slangen JHG, Kerkhoff P (1984) Nitrification inhibitors in agriculture and horticulture: a literature review. Fertil Res 5:1–76

    Article  CAS  Google Scholar 

  • Subbarao GV, Ito O, Sahrawat KL, Berry WL, Nakahara K, Ishikawa T, Watanabe T, Suenaga K, Rondon M, Rao IM (2006) Scope and strategies for regulation of nitrification in agricultural systems – challenges and opportunities. Crit Rev Plant Sci 25:305–335

    Article  Google Scholar 

  • Subbarao GV, Sahrawat KL, Nakahara T, Kishi M, Rao IM, Hash CT, George TS, Srinavasa Rao P, Nardi P, Bonnett D, Berry W, Suenaga K, Lata JC (2013) Biological nitrification inhibition—a novel strategy to regulate nitrification in agricultural systems. In: Sparks D (ed) Advances in agronomy, vol 114. Academic Press, Burlington, pp 249–302

    Google Scholar 

  • Tomiyama H, Ohshima M, Ishii S, Satoh K, Takahashi R, Isobe K, Iwano H, Tokuyama T (2001) Characteristics of newly isolated nitrifying bacteria from rhizoplane of paddy rice. Microb Environ, Japan 16:101–108

    Article  Google Scholar 

  • Williams PH, Jarvis SC, Dixon E (1998) Emission of nitric oxide and nitrous oxide from soil under field and laboratory conditions. Soil Biol Biochem 30:1885–1893

    Article  CAS  Google Scholar 

  • Zakir HAKM, Subbarao GV, Pearse SJ, Gopalakrishnan S, Ito O, Ishikawa T, Kawano N, Nakahara K, Yoshihashi T, Ono H, Yoshida M (2008) Detection, isolation and characterization of a root-exuded compound, methyl 3-(4-hydroxyphenyl) propionate, responsible for biological nitrification inhibition by sorghum (Sorghum bicolor). New Phytol 180:442–451

    Article  CAS  PubMed  Google Scholar 

  • Zerulla W, Barth T, Dressel J, Erhardt K, von Loquenghien H, Pasda K, Radle MG, Wissemeier AH (2001) 3,4-Dimethylpyrazole phosphate: a new nitrification inhibitor for agriculture and horticulture. Biol Fertil Soils 34:79–84

    Google Scholar 

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Authors and Affiliations

  1. Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, 110012, India

    Rajesh Kumar, Balraj S. Parmar, Suresh Walia & Supradip Saha

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  1. Rajesh Kumar
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  2. Balraj S. Parmar
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  3. Suresh Walia
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  4. Supradip Saha
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Correspondence to Rajesh Kumar .

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Editors and Affiliations

  1. Soil Science & Agricultural Chemistry, Institute of Agricultural Sciences, Varanasi, Uttar Pradesh, India

    Amitava Rakshit

  2. Mycology and Plant Pathology, Institute of Agricultural Sciences, Varanasi, Uttar Pradesh, India

    Harikesh Bahadur Singh

  3. Department of Agronomy, Institute of Agricultural Sciences, Varanasi, Uttar Pradesh, India

    Avijit Sen

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Kumar, R., Parmar, B.S., Walia, S., Saha, S. (2015). Nitrification Inhibitors: Classes and Its Use in Nitrification Management. In: Rakshit, A., Singh, H.B., Sen, A. (eds) Nutrient Use Efficiency: from Basics to Advances. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2169-2_8

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