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Biological Control of Pest and Diseases Using Fluorescent Pseudomonads

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Basic and Applied Aspects of Biopesticides

Abstract

Bacteria which are shown to have potential for biological control of destructive diseases are distributed in many genera. Among them, fluorescent pseudomonads are currently considered as the most effective bacteria for biological control of soil and foliar diseases. Fluorescent pseudomonads enhance the plant growth parameters, and hence, they are called plant growth-promoting rhizobacteria (PGPR). PGPR are known to control a wide range of phytopathogens like fungi, bacteria, viruses, insect pests and nematodes, and they are known to control these pathogens by biocontrol mechanism which may be by competition, or antagonism, induction of systemic resistance by these bacteria in the host plant, thereby containing the invading pathogens. For the management of pest and diseases of crop plants, applications of strain mixtures of PGPR formulations perform better than individual strains. Fluorescent pseudomonads showing various modes of action especially rhizosphere colonization, antibiotic production and induction of systemic resistance would certainly be potential biocontrol agents for the management of pest and diseases of crop plants.

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References

  • Audenaert K, Pattery T, Cornelis P, Hofte M (2002) Induction of systemic resistance to Botrytis cinerea in tomato by Pseudomonas aeruginosa 7NSK2: role of salicylic acid, pyochelin and pyocyanin. Mol Plant Microbe Interact 15:1147–1156

    Article  CAS  PubMed  Google Scholar 

  • Bhavani R, Abraham K (2005) Efficacy of selected epiphytic microflora from pod surface against Phytophthora pod rot of cocoa. In: Proceedings of national symposium on biotechnological interventions for improvement of horticultural crops: issues and strategies, Kerala Agricultural University, Kerala, pp 398–400

    Google Scholar 

  • Bora T, Ozaktan H, Gore E, Aslan E (2004) Biological control of Fusarium oxysporum f. sp. melonis by wettable powder formulations of the two strains of Pseudomonas putida. J Phytopathol 152:471–475

    Article  Google Scholar 

  • Broadway RM, Gongora C, Kain WC, Sanderson JA, Monroy JA, Bennett KC, Warner JB, Hoffman MP (1998) Novel chitinolytic enzymes with biological activity against herbivorous insects. J Chem Ecol 24:985–988

    Article  CAS  Google Scholar 

  • Callan NW, Mathre DE, Miller JB (1990) Biopriming seed treatment for biological control of Pythium ultimum pre-emergence damping-off in Sh2 sweet corn. Plant Dis 74:368–372

    Article  Google Scholar 

  • Chakraborty U, Chakraborty B, Basnet M (2006) Plant growth promotion and induction of resistance in Camellia sinensis by Bacillus megaterium. J Basic Microbiol 45:186–195

    Article  Google Scholar 

  • Chen C, Belanger RR, Benhamou N, Paulitz T (2000) Defense enzymes induced in cucumber roots by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol 56:13–23

    Article  CAS  Google Scholar 

  • Dandurand LM, Morra MJ, Chaverra MH, Orser CS (1994) Survival of Pseudomonas spp. in air dried mineral powders. Soil Biol Biochem 26:1423–1430

    Article  CAS  Google Scholar 

  • De Freitas JR, Germida JJ (1992) Growth promotion of winter wheat by fluorescent pseudomonads under growth chamber conditions. Soil Biol Biochem 24:1127–1135

    Article  Google Scholar 

  • Defago G, Berling CH, Burger U, Haas D, Kahr G, Keel C, Voisard C, Wirthner P, Wuthrich B (1990) Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas. In: Hornby D (ed) Biological control of soil borne plant pathogens. CAB International, Wallingford/Oxon, pp 93–108

    Google Scholar 

  • Dubeikovsky AN, Mordukhova EA, Kochethov VV, Polikarpova FV, Boronin AM (1993) Growth promotion of black currant soft wood cuttings by recombinant strain Pseudomonas fluorescens BSP53a synthesizing an increased amount of indole-3-acetic acid. Soil Biol Biochem 25:1277–1281

    Article  Google Scholar 

  • Elad Y, Chet I (1987) Possible role of competition for nutrients in biocontrol of Pythium damping-off by bacteria. Phytopathology 77:190–195

    Article  Google Scholar 

  • Gamliel A, Katan J (1993) Suppression of major and minor pathogens by fluorescent pseudomonads in solarized and non-solarized soils. Phytopathology 83:68–75

    Article  Google Scholar 

  • Glick RB (1995) The enhancement of plant growth promotion by free living bacteria. Can J Microbiol 41:109–117

    Article  CAS  Google Scholar 

  • Gutterson N (1990) Microbial fungicides: recent approaches to elucidating mechanisms. Crit Rev Biotechnol 10:69–91

    Article  Google Scholar 

  • Hagedorn C, Gould WD, Bardinelli TR (1993) Field evaluation of bacterial inoculants to control seedling disease pathogens on cotton. Plant Dis 77:278–282

    Article  Google Scholar 

  • Harish S, Kavino M, Kumar N, Saravanakumar D, Soorianathasundaram K, Samiyappan R (2008) Biohardening with plant growth promoting rhizosphere and endophytic bacteria induces systemic resistance against banana bunchy top virus. Appl Soil Ecol 39:187–200

    Article  Google Scholar 

  • Hebber P, Berge O, Heulin T, Singh SP (1991) Bacterial antagonists of sunflower (Helianthus annuus L.) fungal pathogens. Plant and Soil 133:131–140

    Article  Google Scholar 

  • Hofte M, Boelens J, Verstrete W (1991) Seed protection and promotion of seedling emergence by the plant growth beneficial Pseudomonas strains 7NSK2 and ANP15. Soil Biol Biochem 23:407–410

    Article  Google Scholar 

  • Karthiba L (2010) Molecular and applied biology of microbial consortia mediated resistance in rice plants against leaf folder pest and sheath blight disease. M.Sc. thesis, Tamil Nadu Agricultural University, Coimbatore, India, 180 pp

    Google Scholar 

  • Karthiba L, Saveetha K, Suresh S, Raguchander T, Saravanakumar D, Samiyappan R (2010) PGPR and entomopathogenic fungus bioformulation for the synchronous management of leaffolder pest and sheath blight disease of rice. Pest Manage Sci 66:555–564

    Article  CAS  Google Scholar 

  • Kavino M, Harish S, Kumar N, Saravanakumar D, Damodaran T, Soorianathasundaram K, Samiyappan R (2007) Rhizosphere and endophytic bacteria for induction of systemic resistance of banana plantlets against bunchy top virus. Soil Biol Biochem 39:1087–1098

    Article  CAS  Google Scholar 

  • Kavitha R, Umesha S (2007) Prevalence of bacterial spot in tomato fields of Karnataka and effect of biological seed treatment on disease incidence. Crop Prot 26:991–997

    Article  Google Scholar 

  • Keel C, Schneider U, Maurhofer M, Voisard C, Laville J, Burger U, Wirthner P, Haas D, Defago G (1992) Suppression of root diseases by Pseudomonas fluorescens CHAO: importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol. Mol Plant Microbe Interact 5:4–13

    Article  CAS  Google Scholar 

  • Kelly Cartwright D (1995) Comparison of Pseudomonas species and application techniques for biocontrol of Rhizoctonia stem rot of Poinsettia. Plant Dis 79:309–313

    Article  Google Scholar 

  • Kloepper JW, Schroth MN, Miller TD (1980) Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology 71:1078–1082

    Article  Google Scholar 

  • Lemanceau P, Bakker PAHM, Dekogel WJ, Alabouvette C, Schippers B (1992) Effect of pseudobactin 358 production by Pseudomonas putida WCS358 on suppression of Fusarium wilt of carnation by non pathogenic Fusarium oxysporum Fo47. Appl Environ Microbiol 58:2978–2980

    CAS  PubMed Central  PubMed  Google Scholar 

  • Liu L, Kloepper JW, Tuzun S (1995) Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: duration of protection and effect of host resistance on protection and root colonization. Phytopathology 85:1064–1068

    Article  Google Scholar 

  • Loper JE, Buyer JS (1991) Siderophore in microbial interaction on plant surface. Mol Plant Microbe Interact 4:5–13

    Article  CAS  Google Scholar 

  • M’Piga P, Belanger RR, Paulitz TC, Benhamou N (1997) Increased resistance to Fusarium oxysporum f. sp. radicis lycopersici in tomato plants treated with the endophytic bacterium Pseudomonas fluorescens strain 63–28. Physiol Mol Plant Pathol 50:301–320

    Article  Google Scholar 

  • Maurhofer M, Keel C, Haas D, Defago G (1994) Pyoluteorin production by Pseudomonas fluorescens strain CHAO is involved in the suppression of Pythium damping-off of cress but rot of cucumber. Eur J Plant Pathol 100:221–232

    Article  CAS  Google Scholar 

  • Mazzola M, Cook RJ, Thomashow LS, Weller DM, Pierson LS (1992) Contribution of phenazine antibiotic biosynthesis to the ecological competence of fluorescent pseudomonads in soil habitats. Appl Environ Microbiol 58:2616–2624

    CAS  PubMed Central  PubMed  Google Scholar 

  • Meena B, Marimuthu T (2012) Effect of application methods of Pseudomonas fluorescens for the late leaf spot of groundnut management. J Biopest 5:14–17

    Google Scholar 

  • Meena B, Radhajeyalakshmi R, Marimuthu T, Vidhyasekaran P, Doraiswamy S, Velazhahan R (2000) Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens. J Plant Dis Prot 107:514–527

    CAS  Google Scholar 

  • Meena B, Marimuthu T, Vidhyasekaran P, Velazhahan R (2001) Biological control of root rot of groundnut with antagonistic Pseudomonas fluorescens strains. J Plant Dis Prot 108:369–381

    CAS  Google Scholar 

  • Meena B, Radhajeyalakshmi R, Marimuthu T, Vidhyasekaran P, Velazhahan R (2002) Biological control of groundnut late leaf spot and rust by seed and foliar applications of a powder formulation of Pseudomonas fluorescens. Bio Sci Technol 12:195–204

    Article  Google Scholar 

  • Meena B, Marimuthu T, Velazhahan R (2006) Role of fluorescent pseudomonads in plant growth promotion and biological control of late leaf spot of groundnut. Acta Phytopathol Entomol Hung 4:203–212

    Article  Google Scholar 

  • Murphy JF, Zehnder GW, Schuster DJ, Sikora EJ, Polston JE, Kloepper JW (2000) Plant growth promoting rhizobacterial mediated protection in tomato against tomato mottle virus. Plant Dis 84:779–784

    Article  Google Scholar 

  • Muthamilan M (1994) Management of diseases of chickpea and rice using fluorescent pseudomonads. PhD thesis, Tamil Nadu Agricultural University, Coimbatore, India, 182 pp

    Google Scholar 

  • Nagaraj KM, Bhaskaran R, Velazhahan R (2004) Involvement of secondary metabolites and extra cellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen. Microbiol Res 159:73–81

    Article  Google Scholar 

  • Nakkeeran S, Dilantha Fernando WG, Siddiqui A (2005) Plant growth promoting rhizobacteria. In: Siddiqui ZA (ed) PGPR: biocontrol and biofertilization. Springer, Dordrecht, pp 257–296

    Google Scholar 

  • Nelson LM (2004) Plant growth promoting rhizobacteria (PGPR): prospects for new inoculants. Plant Manage Netw 10:301–305

    Google Scholar 

  • Otsu Y, Matsuda Y, Mori H, Ueki H, Nakajima T, Fujiwara K, Matsumoto M, Azuma N, Kakutani K, Nonomura T, Sakuratami Y, Shinogi T, Tosa Y, Mayama S, Toyode H (2004) Stable phyllosphere colonization by entomopathogenic bacterium Pseudomonas fluorescens KPM-018P and biological control of phytophagous ladybird beetles Epilachna vigintioctopunctata (Coleoptera: Coccinellidae). Bio Sci Technol 14:427–439

    Article  Google Scholar 

  • Pal KK, Dey R, Bhatt DM, Chauhan S (1999) Enhancement of groundnut growth and yield by plant growth promoting rhizobacteria. Int Arachis Newsl 19:51–53

    Google Scholar 

  • Paul D, Sarma YR (2006) Antagonistic effects of metabolites of P. fluorescens strains on the different growth phases of Phytophthora capsici, root rot pathogen of black pepper (Piper nigrum L.). Arch Phytopathol Plant Prot 39:113–118

    Article  CAS  Google Scholar 

  • Pechy-Tarr M, Bruck DJ, Maurhofer M, Fischer E, Vogne C, Henkels MD, Donahue KM, Grunder J, Loper JE, Keel C (2008) Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environ Microbiol 10:2368–2386

    Article  CAS  PubMed  Google Scholar 

  • Rabindran R (1994) Biological control of rice sheath blight caused by Rhizoctonia solani and blast caused by Pyricularia oryzae using Pseudomonas fluorescens. PhD thesis, Tamil Nadu Agricultural University, Coimbatore, India, 179 pp

    Google Scholar 

  • Rabindran R, Vidhyasekaran P (1996) Development of a formulation of Pseudomonas fluorescens PfALR2 for management of rice sheath blight. Crop Prot 15:715–721

    Article  Google Scholar 

  • Radjacommare R, Kandan A, Nandakumar R, Samiyappan R (2004) Association of the hydrolytic enzyme chitinase against Rhizoctonia solani in rhizobacteria-treated rice plants. J Phytopathol 152:365–370

    Article  CAS  Google Scholar 

  • Rajendran L, Samiyappan R, Raguchander T, Saravanakumar D (2007) Endophytic bacteria mediate plant resistance against cotton bollworm. J Plant Interact 2:1–10

    Article  CAS  Google Scholar 

  • Ramamoorthy V, Viswanathan R, Raguchander T, Prakasam V, Samiyappan R (2001) Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pest and diseases. Crop Prot 20:1–11

    Article  CAS  Google Scholar 

  • Ramamoorthy V, Raguchander T, Samiyappan R (2002) Enhancing resistance of tomato and hot pepper to Pythium diseases by seed treatment with fluorescent pseudomonads. Eur J Plant Pathol 108:429–441

    Article  CAS  Google Scholar 

  • Samiyappan R (1988) Biological control of black gram root rot caused by Macrophomina phaseolina (Tassi) Goid. PhD thesis, Tamil Nadu Agricultural University, Coimbatore, India, 184 pp

    Google Scholar 

  • Saravanakumar D, Vijayakumar C, Kumar N, Samiyappan R (2007) PGPR-induced defense responses in the tea plant against blister blight disease. Crop Prot 26:556–565

    Article  Google Scholar 

  • Shanahan P, O’Sullivan DJ, Simpson P, Glennon JD, O’Gara F (1992) Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl Environ Microbiol 58:353–358

    CAS  PubMed Central  PubMed  Google Scholar 

  • Sreenivasulu B, Krishnakumar KV, Aruna K, Lakshmi MV, Rao DVR (2006) Biointensive IDM approach against basal stem rot and stem bleeding disease of coconut. J Plant Crops 34:502–507

    Google Scholar 

  • Stutz EW, Defago G, Keran H (1986) Naturally occurring fluorescent pseudomonads involved in suppression of black root rot of tobacco. Phytopathology 76:181–185

    Article  Google Scholar 

  • Suslow TV, Kloepper JW, Schroth MN, Burr TJ (1979) Beneficial bacteria enhance plant growth. Calif Agric 33:15–17

    Google Scholar 

  • Thomashow LS, Weller DM (1990) Application of fluorescent pseudomonads to control root diseases of wheat and some mechanisms of disease suppression. Soil Biol Biochem 21:109–122

    Google Scholar 

  • Thomashow LS, Bonsall RF, Weller DM (1997) Antibiotic production by soil and rhizosphere microbes in situ. In: Hurst CJ, Knudsen GR, McInerney MJ, Stetzenbach LD, Walter MV (eds) Manual of environmental microbiology. ASM Press, Washington, DC, pp 493–499

    Google Scholar 

  • Tian B, Yang J, Zhang K (2007) Bacteria used in the biological control of plant-parasitic nematodes: populations, mechanisms of action, and future prospects. FEMS Microbiol Ecol 61:197–213

    Article  CAS  PubMed  Google Scholar 

  • Tosi L, Zazzerini A (1994) Evaluation of some fungi and bacteria for potential control of safflower rust. J Phytopathol 142:131–140

    Article  Google Scholar 

  • Uppal AK, El Hadrami A, Adam R, Tenuta M, Daayf F (2008) Biological control of potato Verticillium wilt under controlled and field conditions using selected bacterial antagonists and plant extracts. Biol Control 44:90–100

    Article  Google Scholar 

  • Van Loon LC, Pierpoint WS, Boller T, Conejero V (1994) Recommendations for naming plant pathogenesis-related proteins. Plant Mol Biol Rep 12:245–264

    Article  Google Scholar 

  • Van Peer R, Niemann GJ, Schippers B (1991) Induced resistance and phytoalexin accumulation in biological control of Fusarium wilt of carnation by Pseudomonas sp. strain WCS417r. Phytopathology 81:728–734

    Article  Google Scholar 

  • Verhagen BWM, Glazebrook J, Zhu T, Chang HS, Van Loon LC, Pieterse CMJ (2004) The transcriptome of rhizobacteria-induced systemic resistance in Arabidopsis. Mol Plant Microbe Interact 17:895–908

    Article  CAS  PubMed  Google Scholar 

  • Verhagen BWM, Aziz PT, Couderchet M, Hofte M, Aziz A (2009) Pseudomonas spp.-induced systemic resistance to Botrytis cinerea is associated with induction and priming of defence responses in grapevine. J Exp Bot 61:249–260

    Article  Google Scholar 

  • Vidhyasekaran P, Rabindran R, Muthamilan M, Nayar K, Rajappan K, Subramanian N, Vasumathi K (1997) Development of powder formulation of Pseudomonas fluorescens for control of rice blast. Plant Pathol 46:291–297

    Article  Google Scholar 

  • Viswanathan R, Samiyappan R (2001) Antifungal activity of chitinase produced by some fluorescent pseudomonads against Colletotrichum falcatum Went causing red rot disease in sugarcane. Microbiol Res 155:309–314

    Article  CAS  PubMed  Google Scholar 

  • Vivekananthan R, Ravi M, Ramanathan A, Samiyappan R (2004) Lytic enzymes induced by Pseudomonas fluorescens and other biocontrol organisms mediate defence against the anthracnose pathogen in mango. World J Microbiol Biotechnol 20:235–244

    Article  CAS  Google Scholar 

  • Vleesschauwer D, Djavaheri M, Bakker PAHM, Hofte M (2008) Pseudomonas fluorescens WCS374r-Induced systemic resistance against Magnaporthe oryzae is based on pseudobactin-mediated priming for a salicylic acid-repressible multifaceted defense response. Plant Physiol 148:1996–2012

    Article  PubMed Central  PubMed  Google Scholar 

  • Voisard C, Keel C, Hass D, Defago G (1989) Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under gnotobiotic conditions. EMBO J 8:351–358

    CAS  PubMed Central  PubMed  Google Scholar 

  • Wei G, Kloepper JW, Tuzun S (1991) Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth promoting rhizobacteria. Phytopathology 81:1508–1512

    Article  Google Scholar 

  • Wei G, Kloepper JW, Tuzun S (1996) Induced systemic resistance to cucumber diseases and increased plant growth by plant growth promoting rhizobacteria under field conditions. Phytopathology 86:221–224

    Article  Google Scholar 

  • Weller DM (1988) Biological control of soil borne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407

    Article  Google Scholar 

  • Weller DM, Raaijmakers JM, McSpadden Gardener BB, Thomashow LS (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu Rev Phytopathol 40:309–348

    Article  CAS  PubMed  Google Scholar 

  • Weststeijn WA (1990) Fluorescent pseudomonads isolate E11-2 as biological agent for Pythium root rot in tulips. Neth J Plant Pathol 96:262–272

    Article  Google Scholar 

  • Williams GE, Asher MJC (1996) Selection of rhizobacteria for the control of Pythium ultimum and Aphanomyces cochlioides on sugarbeet seedlings. Crop Prot 15:479–486

    Article  Google Scholar 

  • Wilson H, Epton HAS, Sigee DC (1992) Biological control of fire blight of Hawthorn with fluorescent Pseudomonas spp. under protected conditions. J Phytopathol 136:16–26

    Article  Google Scholar 

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Meena, B. (2014). Biological Control of Pest and Diseases Using Fluorescent Pseudomonads. In: Sahayaraj, K. (eds) Basic and Applied Aspects of Biopesticides. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1877-7_2

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