Abstract
Insects have sophisticated learning abilities despite the relative simplicity of their central neural systems, which consist of small numbers of neurons as compared to vertebrates. Among insects, crickets (Gryllus bimaculatus) exhibit the most robust olfactory learning and memory. In this chapter, we describe protocols for classical conditioning and memory retention tests in crickets. Crickets are individually trained to associate an odor (conditioned stimulus) with water reward (appetitive unconditioned stimulus). To evaluate the effect of training, relative preference between the conditioned odor and a control odor is tested before and after training. We describe the methodology of olfactory conditioning in detail to help researchers who are interested in using crickets to study learning and memory.
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References
Avarguès-Weber A, Deisig N, Giurfa M (2011) Visual cognition in social insects. Annu Rev Entomol 56:423–443
Balderrama N (1980) One trial learning in the American cockroach, Periplaneta americana. J Insect Physiol 26:499–504
Bitterman ME, Menzel R, Fietz A, Schafer S (1983) Classical conditioning of proboscis extension in honeybees (Apis mellifera). J Comp Psychol 97:107–119
Daly KC, Smith BH (2000) Associative olfactory learning in the moth Manduca sexta. J Exp Biol 203:2025–2038
Dupuy F, Sandoz JC, Giurfa M, Josens R (2006) Individual olfactory learning in Camponotus ants. Anim Behav 72:1081–1091
Giurfa M (2007) Behavioral and neural analysis of associative learning in the honeybee: a taste from the magic well. J Comp Physiol A 193:801–824
Giurfa M, Sandoz JC (2012) Invertebrate learning and memory: fifty years of olfactory conditioning of the proboscis extension response in honeybees. Learn Mem 19:54–66
Matsumoto Y, Mizunami M (2000) Olfactory learning in the cricket Gryllus bimaculatus. J Exp Biol 203:2581–2588
Matsumoto Y, Mizunami M (2002a) Temporal determinants of olfactory long-term retention in the cricket Gryllus bimaculatus. J Exp Biol 205:1429–1437
Matsumoto Y, Mizunami M (2002b) Lifetime olfactory memory in the cricket Gryllus bimaculatus. J Comp Physiol A 188:295–299
Matsumoto Y, Mizunami M (2004) Context-dependent olfactory learning in an insect. Learn Mem 11:288–293
Matsumoto Y, Mizunami M (2005) Formation of long-term olfactory memory in the cricket Gryllus bimaculatus. Chem Senses 30(suppl 1):i299–i300
Matsumoto Y, Mizunami M (2006) Olfactory memory capacity of the cricket Gryllus bimaculatus. Biol Lett 2:608–610
Matsumoto Y, Noji S, Mizunami M (2003) Time course of protein synthesis-dependent phase of olfactory memory in the cricket Gryllus bimaculatus. Zool Sci 20:409–416
Matsumoto Y, Unoki S, Aonuma H, Mizunami M (2006) Critical role of nitric oxide-cGMP cascade in the formation of cAMP-dependent long-term memory. Learn Mem 13:35–44
Matsumoto Y, Hatano A, Unoki S, Mizunami M (2009) Stimulation of the cAMP system by the nitric oxide-cGMP system underlying the formation of long-term memory in an insect. Neurosci Lett 467:81–85
Matsumoto Y, Hirashima D, Mizunami M (2013a) Analysis and modeling of neural processes underlying sensory pre-conditioning. Neurobiol Learn Mem 101:103–113
Matsumoto Y, Hirashima D, Terao K, Mizunami M (2013b) Roles of NO signaling in long-term memory formation in visual learning in an insect. PLoS One 8(7):e68538
Menzel R (1999) Memory dynamics in the honeybee. J Comp Physiol A 185:323–340
Mizunami M, Yokohari F, Takahata M (2004) Further exploration into the adaptive design of the arthropod “microbrain”: I. Sensory and memory-processing systems. Zool Sci 21:1141–1151
Mizunami M, Unoki S, Mori Y, Hirashima D, Hatano A, Matsumoto Y (2009) Roles of octopaminergic and dopaminergic neurons in appetitive and aversive memory recall in an insect. BMC Biol 7:46
Mizunami M, Nemoto Y, Terao K, Hamanaka Y, Matsumoto Y (2014) Roles of calcium/calmodulin-dependent kinase II in long-term memory formation in crickets. PLoS One 9(9):e107442
Nakatani Y, Matsumoto Y, Mori Y, Hirashima D, Nishino H, Arikawa K, Mizunami M (2009) Why the carrot is more effective than the stick: different dynamics of punishment memory and reward memory and its possible biological basis. Neurobiol Learn Mem 92:370–380
Sandoz JC (2011) Behavioral and neurophysiological study of olfactory perception and learning in honeybees. Front Syst Neurosci 5:98. doi:10.3389/fnsys.2011.00098
Takahashi T, Hamada A, Miyawaki K, Matsumoto Y, Mito T, Noji S, Mizunami M (2009) Systemic RNA interference for the study of learning and memory in an insect. J Neurosci Methods 179:9–15
Terao K, Matsumoto Y, Mizunami M (2015) Conserved computational mechanisms underlying associative learning in insects and mammals. Sci Rep 5:8929
Tully T, Quinn WG (1985) Classical conditioning and retention in normal and mutant Drosophila melanogaster. J Comp Physiol A 157:263–277
Unoki S, Matsumoto Y, Mizunami M (2005) Participation of octopaminergic reward system and dopaminergic punishment system in insect olfactory learning revealed by pharmacological study. Eur J Neurosci 22:1409–1416
Unoki S, Matsumoto Y, Mizunami M (2006) Roles of octopaminergic and dopaminergic neurons in mediating reward and punishment signals in insect visual learning. Eur J Neurosci 24:2031–2038
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Matsumoto, Y., Matsumoto, C.S., Mizunami, M. (2017). Protocols for Olfactory Conditioning Experiments. In: Horch, H., Mito, T., Popadić, A., Ohuchi, H., Noji, S. (eds) The Cricket as a Model Organism. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56478-2_17
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DOI: https://doi.org/10.1007/978-4-431-56478-2_17
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