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Cell Culture

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Cell Therapy Against Cerebral Stroke

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Abstract

Cell production under Good Manufacturing Practice (GMP) protocol is mandatory for the proper application of therapeutic cells in clinical settings. If cells are produced under GMP conditions, chemically defined conditions and a controlled environment would be ensured. However, such practices do not specify the use of animal-derived or xenogeneic recombinant supplements, which might raise some concern for clinical-grade cell preparations. At the very least, information of these materials should be provided to the patients treated with cell therapy to ensure proper understanding and informed assent. Therefore, in this chapter, the conventional cell culture methods employed for cell preparation (isolation, expansion, and/or derivation) are discussed, with a particular focus on each of the cell types employed in clinical trials of cell therapy against cerebral stroke.

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Authors and Affiliations

  1. Department of Neurosurgery, Hokkaido University Graduate School of Medicine, Kita 15, Nishi 7, Kita-ku, Sapporo, 060-8638, Japan

    Masaki Ito & Kiyohiro Houkin

  2. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, 94305-5487, USA

    Masaki Ito

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  1. Masaki Ito
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  2. Kiyohiro Houkin
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Correspondence to Masaki Ito .

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  1. Hokkaido University Graduate School of M, Department of Neurosurgery, Sapporo, Hokkaido, Japan

    Kiyohiro Houkin

  2. Okayama University Graduate School of Me, Department of Neurology, Okayama, Okayama, Japan

    Koji Abe

  3. Graduate School of Medicine and Pharmace, Department of Neurosurgery, Toyama, Toyama, Japan

    Satoshi Kuroda

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Ito, M., Houkin, K. (2017). Cell Culture. In: Houkin, K., Abe, K., Kuroda, S. (eds) Cell Therapy Against Cerebral Stroke. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56059-3_5

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