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Ovarian Tissue Cryopreservation: Ovarian Cortical Tissue Vitrification

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Gonadal Tissue Cryopreservation in Fertility Preservation

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Abstract

Vitrification has become common practice in cryopreservation of embryo, blastocyst, oocytes, and sperm. Freezing of ovarian tissue has been studied since the late 1990s, and in 2004, Donnez et al. reported that live births could be achieved with this method. Reports worldwide have indicated that slow freezing is a common method of cryopreservation. Successive reports have emerged since 2005 describing ovarian tissue cryopreservation by vitrification in animal experiments and using human tissue; however, there have been no reports to date in which vitrification was clinically applied and live births were successfully achieved. We have been conducting studies through pre-clinical tests using Cynomolgus monkey (Macaca fascicularis) since 2006, and as a result, at present in June, 2016, we have achieved ovarian tissue cryopreservation in 229 cases and 2 live births following thawed transplantation. This article outlines the research findings so far regarding ovarian tissue cryopreservation by vitrification; additionally, it describes our ovarian cortical tissue vitrification method.

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References

  1. Hoagland H, Pincus G (1942) Revival of mammalian sperm after immersion in liquid nitrogen. J Gen Physiol 25(3):337–344

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. The Practice Committee of the American Society for Reproductive Medicine (2014) Ovarian tissue cryopreservation: a committee opinion. Fertil Steril 101(5):1237–1243

    Article  Google Scholar 

  3. Cakmak H, Audra Katz RN, Cedars MI, Rosen MP (2013) Effective method for emergency fertility preservation: random-start controlled ovarian stimulation. Fertil Steril 100(6):1673–1680

    Article  PubMed  Google Scholar 

  4. Oktay K, Nugent D, Newton H, Salha O, Chatterigee P, Gosden RG (1997) Isolation and characterizeation of primordiall follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 3:481–486

    Article  Google Scholar 

  5. Oktay K, Newton H, Aubanrd Y, Salha O, Gosden RG (1998) Cryopreservation of immature human oocytes and ovarian tissue: an emerging technology? Fertil Steril 59(1):1–7

    Article  Google Scholar 

  6. Newthon H, Aubard Y, Rutherford A, Sharma V, Gosden R (1996) Low temperature storage and grafting of human ovarian tissue. Hum Reprod 11(7):1487–1491

    Article  Google Scholar 

  7. Hovatta O, Silye R, Krausz T, Abir R, Margara R, Trew G, Lass A, Winston RM (1996) Cryopreservation of human ovarian tissue using dimethylsulphoxide and propanediol-sucrose as cryoprotectants. Hum Reprod 11(6):1268–1272

    Article  CAS  PubMed  Google Scholar 

  8. Donnez J, Bassil S (1998) Indications of cryopreservation of ovarian tissue. Hum Reprod Update 4(3):248–259

    Article  CAS  PubMed  Google Scholar 

  9. Meirow D, Ben Yehuda D, Prus D, Poliack A, Schenker JG, Rachmilewiz EA, Lewin A (1998) Ovarian tissue banking in patients with Hodgkin’s disease: is it safe? Fertil Steril 69(6):996–998

    Article  CAS  PubMed  Google Scholar 

  10. Oktay K, Buyuk E, Veeck L, Zaninovic N, Xu K, Takeuchi T, Opsahl M, Rosenwaks Z (2004) Embryo development after heterotopic transplantation of cryopreserved ovarian tissue. Lancet 363:837–840

    Article  PubMed  Google Scholar 

  11. Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, Van Langendonckt A (2004) Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet 364:1405–1410

    Article  CAS  PubMed  Google Scholar 

  12. Callejo J, Salvador C, Miralles A et al (2001) Long-term ovarian function evaluation after autografting by implantation with fresh and frozen–thawed human ovarian tissue. J Clin Endocrinol Metab 8:4489–4494

    Article  Google Scholar 

  13. Fabbri R (2006) Cryopreservation of human oocytes and ovarian tissue. Cell Tissue Bank 7:113–122

    Article  PubMed  Google Scholar 

  14. Gook DA, McCully BA, Edgar DH, McBain JC (2001) Development of antral follicles in human cryopreserved ovarian tissue following xenografting. Hum Reprod 16:417–422

    Article  CAS  PubMed  Google Scholar 

  15. Gook DA, Edgar DH, Borg J et al (2003) Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovar- ian tissue following xenografting. Hum Reprod 18:1772–1781

    Article  CAS  PubMed  Google Scholar 

  16. Gook DA, Edgar DH, Borg J et al (2005) Diagnostic assess- ment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 26:72–78

    Google Scholar 

  17. Kim SS, Hwang IT, Lee HC, Kim DH (2003) Ovarian function after autotransplantation of frozen thawed human ovarian tissue: an experience of human ovarian grafting into two eterotopic sites. Fertil Steril 80:94–95

    Article  Google Scholar 

  18. Kim SS, Hwang IT, Lee HC (2004) Heterotopic autotrans- plantation of cryobanked human ovarian tissue as a strategy to restore ovarian function. Fertil Steril 82:930–932

    Article  PubMed  Google Scholar 

  19. Kim SS, Lee WS, Chung MK et al (2009) Long-term ovarian function and fertility after heterotopic autotransplantation of cryobanked human ovarian tissue: 8-year experience in cancer patients. Fertil Steril 91:2349–2354

    Article  PubMed  Google Scholar 

  20. Meirow D, Levron J, Eldar-Geva T et al (2005) Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med 353:318–321

    Article  CAS  PubMed  Google Scholar 

  21. Meirow D, Levron J, Eldar-Geva T et al (2007) Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth. Fertil Steril 87:418.e7–418.e15

    Article  Google Scholar 

  22. Von Wolff M, Donnez J, Hovatta O et al (2009) Cryopreserva- tion and autotransplantation of human ovarian tissue prior to cytotoxic therapy – a technique in its infancy but already successful in fertility preservation. Eur J Cancer 45:1547–1553

    Article  Google Scholar 

  23. Stoop D, Cobo A, Silber S (2014) Fertility preservation for age-related fertileity decline. Lancet 384:1311–1319

    Article  PubMed  Google Scholar 

  24. Donnez J, Dolmans MM, Pellicer A, Diaz-Garcia C, Ernst E, Macklon KT, Andersen CY (2015) Fertility preservation for age-related fertility decline. Lancet 385:506–507

    Article  PubMed  Google Scholar 

  25. von Wolff M, Montag M, Dittrich R, Denshlag D, Nawroth F, Lawrenz B (2011) Fertility preservation in women-a practiceal guide to preservation techniques and therapeutic strategyes in breast cancer, Hodgkin’s lymphoma and borderline ovaryian tumors by the fertility preservation network FertiPROTEKT. Arch Gynecol Obstet 284:427–435

    Article  Google Scholar 

  26. ISFP Practiee Committee, Kim SS, Donnez J, Barri P, Pellicer A, Pasquale P, Rosenwaks Z, Nagy P, Falcone T, Andersen C, Hovatta O, Wallace H, Meirow D, Gook D, Kim SH, Tzeng C-R, Suzuki S, Ishizuka B, Dolmans M-M (2012) Recommendations for fertileity preservation in patients with lymphoma, leukemia, and breast cancer. J Assist Reprod Genet 29:465–468

    Article  Google Scholar 

  27. Loren AW, Mangu PB, Beck LN, Brennan L, Magdalinski AJ, Partridge AH, Quinn G, Wallace WH, Oktay K (2013) Fertility preservation for patients with cancer: American Society of Clinical Oncology Clinical Practice Guideline Update. J Clin Oncol 311(19):2500–2511

    Article  Google Scholar 

  28. Featured Update to the NCCN Guidelines (2014) Adolescent and Young Adult Oncology, Ver 2.2014. J Natl Compr Cancer Netw. 12(1):21–32

    Google Scholar 

  29. Yeoman RR, Wolf DP, Lee DM (2005) Coculture of monkey ovarian tissue increases survival after vitrification and slow-rate freezing. Fertil Steril 83:1248–1254

    Article  PubMed  Google Scholar 

  30. Gandolfi F, Paffoni A, Papasso Brambilla E, Bonetti S, Brevini TAL, Pharm D, Ragni G (2006) Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models. Fertil Steril 85(1):1150–1156

    Article  PubMed  Google Scholar 

  31. Ting AY, Yeoman RR, Lawson S, Zelinski MB (2011) In vitro development of secondary follicles from cryopreserved rhesus macaque ovarian tissue after slow-rate-freeze or vitrification. Hum Reprod 26(9):2461–2472

    Article  PubMed  PubMed Central  Google Scholar 

  32. Keros V, Xella S, Hultenby K, Pettersson K, Sheikhi M, Volpe A, Hreinsson J, Hovatta O (2009) Vitrification versus controlled-rate freezing in cryopreserveation of human ovarian tissue. Hum Reprod 24(7):1670–1683

    Article  CAS  PubMed  Google Scholar 

  33. Sheikhi M, Hultendby K, Niklasson B, Lundqvist M, Hobatta O (2011) Clinical grade vitrification: an ultrastructural analysis of follicles and stroma in vitrified tissue. Hum Reprod 26(3):594–603

    Article  PubMed  PubMed Central  Google Scholar 

  34. Amorim CA, Curaba M, Van Langendonckt A, Dolmans M-M, Donnez J (2011) Vitrification as analternative means of cryopreserveing ovarian tissue. Repro Biomed On 23:160–186

    Article  Google Scholar 

  35. Hashimoto S, Suzuki N, Yamanaka M, Hosoi Y, Ishizuka B, Morimoto Y (2010) Effects of vitrification solutions and equilibrateon times on the morphology of cynomolgus ovaryan tissue. Repro Bio Online 21:501–509

    Article  Google Scholar 

  36. Igarashi S, Suzuki N, Hashimoto S, Takae S, Takenoshita M, Hosoi Y, Morimoto Y, Ishizuka B (2010) Heterotopic autotransplantation of ovarian cortex in cynomolgus monkeys. Hum Cell 23(1):26–34

    PubMed  Google Scholar 

  37. Ishimori H, Takahashi Y, Kanagawa H (1992) Factors affecting survival of mouse blastocysts vitrified by a mixture of ethylene glycol and dimethyl sulfoxide. Theriogenology 38:1175–1185

    Article  CAS  PubMed  Google Scholar 

  38. Suzuki N, Hashimoto S, Igarashi S, Takae S, Yamanaka M, Yamochi T, Takenosita M, Hosoi Y, Morimoto Y, Ishizuka B (2012) Assesment of long-term function of heterotopic transplants of vitrified ovarian tissue in cynomolgus monkeys. Hum Reprod 27(8):2420–2429

    Article  PubMed  Google Scholar 

  39. Hashimoto S, Suzuki N, Amo A, Yamochi T, Hosoi Y, Morimoto Y (2013) Good thermally conducting material supports follicle morphologyes of procine ovaries cryopreserved with ultrarapid vitrifycation. J Reprod Dev 59(5):496–499

    Article  PubMed  PubMed Central  Google Scholar 

  40. Kawamura K, Cheng Y, Suzuki N, Deguchi M, Sato Y, Takae S, Ho CH, Kawamura N, Tamura M, Hashimoto S, Sugishita Y, Morimoto Y, Hosoi Y, Yoshioka N, Ishizuka B, Hsueh AJ (2013) Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci 110(43):17474–17479

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Suzuki N, Yoshioka N, Takae S, Sugishita Y, Tamura M, Hashimoto S, Morimoto Y, Kawamura K (2015) Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency. Hum Reprod 30:608–615

    Article  PubMed  Google Scholar 

  42. Revel A, Elami A, Bor A, Yavin S, Natan Y, Arav A (2004) Whole sheep ovary cryopreservation and transplantation. Fertil Steril 82:1714–1715

    Article  PubMed  Google Scholar 

  43. Courbiere B, Massardier J, Salle B, Mazoyer C, Guerin JF, Lornage J (2005) Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification. Fertil Steril 84:1065–1071

    Article  PubMed  Google Scholar 

  44. Courbiere B, Odagescu V, Baudot A, Massardier J, Mazoyer C, Salle B, Lornage J (2006) Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols. Fertil Steril 86:1243–1251

    Article  PubMed  Google Scholar 

  45. Courbiere B, Caquant L, Mazoyer C, Franck M, Lornage J, Salle B (2009) Difficulties improving ovarian functional recovery by microvascular transplantation and whole ovary vitrification. Fertil Steril 91:2697–2706

    Article  PubMed  Google Scholar 

  46. Silber S, Kagawa N, Kuwayama M, Gosden R (2010) Duration of fertileity after fresh and frozen ovary transplantation. Fertil Steril 94:2191–2196

    Article  PubMed  Google Scholar 

  47. Kikuchi I, Kagawa N, Silber S, Kuwayama M, Takehara Y, Aono F, Kumakiri J, Kato O, Takeda S (2013) Oophorectomy for fertility preservation via reduced-port laparoscopic surgery. Surg Innov 20(3):219–224

    Article  PubMed  Google Scholar 

  48. Kagawa N, Kikuchi I, Kuwayama M (2015) Ovarian tissue vitrification for fertility preservation. Vitrification in Assisted Reproduction-Springer. 43–49

    Google Scholar 

  49. Bielanski A (2014) Biosafety embryo and semen cryopreservation, storage, management and transport. Adv Exp Med Biol 753:429–465

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa, 216-8511, Japan

    Yodo Sugishita, Suguru Igarashi, Mariko Nakajima, Chie Nishijima, Seido Takae, Yuki Horage, Kazuhiro Kawaura & Nao Suzuki

  2. IVF Namba Clinic, Osaka, Japan

    Shu Hashimoto, Takayuki Yamochi & Yoshiharu Morimoto

  3. Institute of Advanced Technology, Kinki University, Wakayama, Japan

    Yoshihiko Hosoi

  4. Horac Grand Front Osaka Clinic, Osaka, Japan

    Yoshiharu Morimoto

Authors
  1. Yodo Sugishita
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  2. Shu Hashimoto
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  3. Takayuki Yamochi
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  4. Suguru Igarashi
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  5. Mariko Nakajima
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  6. Chie Nishijima
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  7. Seido Takae
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  8. Yuki Horage
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  9. Kazuhiro Kawaura
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  10. Yoshihiko Hosoi
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  11. Yoshiharu Morimoto
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  12. Nao Suzuki
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Corresponding author

Correspondence to Nao Suzuki .

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Editors and Affiliations

  1. Department of Obstetrics and Gynecology, St. Marianna University School of Medici, Kawasaki, Kanagawa, Japan

    Nao Suzuki

  2. Societe de Recherche pour l'Inferti, Brussels, Belgium

    Jacques Donnez

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Sugishita, Y. et al. (2016). Ovarian Tissue Cryopreservation: Ovarian Cortical Tissue Vitrification. In: Suzuki, N., Donnez, J. (eds) Gonadal Tissue Cryopreservation in Fertility Preservation. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55963-4_5

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  • DOI: https://doi.org/10.1007/978-4-431-55963-4_5

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  • Online ISBN: 978-4-431-55963-4

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