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High-Throughput Cell Assembly Featuring Heterogeneous Hydrogels Produced by Using Microfluidic Devices

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Hyper Bio Assembler for 3D Cellular Systems

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Abstract

We describe microfluidic devices developed for producing heterogeneous hydrogel materials including sandwich-type hydrogel fibers, hydrogel fibers having a highly complex cross-sectional morphology, stripe-patterned hydrogel sheets, and yarn-ball-shaped hydrogel beads. Cells encapsulated within these hydrogel materials exhibit behaviors that are distinct from those of cells examined using conventional cell-culture techniques. The cells are rapidly encapsulated in the hydrogel materials, and the cell-containing materials obtained could function as unit structures in constructing large tissues. Here, we also briefly discuss the use of hydrogel-based microfluidic devices in the preparation of multilayer blood-vessel models.

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Acknowledgments

We would like to thank Dr. S. Sugaya, Dr. R. Utoh, Mr. M. Iwase, Mr. Y. Kitagawa, Ms. E. Yamada, Mr. Y. Nagamuna, Ms. A. Kobayashi, Ms. A. Miyama, Mr. Y. Yajima, Ms. A. Hori for their contribution in conducting experiments. This study was supported in part by Grants-in-aid for Scientific Research (KAKENHI 23106007, 23700554, and 30546784) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Authors and Affiliations

  1. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, 263-8522, Inage-ku, Chiba, Japan

    Masumi Yamada & Minoru Seki

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  1. Masumi Yamada
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  2. Minoru Seki
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Correspondence to Masumi Yamada .

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Editors and Affiliations

  1. Department of Systems Innovation, Osaka University, Toyonaka, Japan

    Tatsuo Arai

  2. Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya, Japan

    Fumihito Arai

  3. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan

    Masayuki Yamato

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Yamada, M., Seki, M. (2015). High-Throughput Cell Assembly Featuring Heterogeneous Hydrogels Produced by Using Microfluidic Devices. In: Arai, T., Arai, F., Yamato, M. (eds) Hyper Bio Assembler for 3D Cellular Systems. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55297-0_8

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  • DOI: https://doi.org/10.1007/978-4-431-55297-0_8

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  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-55296-3

  • Online ISBN: 978-4-431-55297-0

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