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Rapid Single Cell Printing by Piezoelectric Inkjet Printer

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Engineered Cell Manipulation for Biomedical Application

Part of the book series: Nanomedicine and Nanotoxicology ((NANOMED))

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Abstract

Tissue engineering is rapidly developing to assist in the treatment of organ loss or tissue damage. On the other hand, the relatively long process of drug development may also need in vitro fabricated organ for drug testing, which opens up faster door to development process. Several methods and technologies have been introduced as valuable tools for tissue engineering, and inkjet printing stands out as the “scaffold-less” tissue engineering tools starting from the early 2000s, although the scaffold-less feature is still debatable. Furthermore, this technology was also used to encapsulate single cells in the fluid droplet, thus opening up the possibility for isolation of single cells in the open space and better spatiotemporal control of cell printing at individual level. Combined with the high-frequency droplet ejection capability of inkjet printer, high-throughput single cells array and high-speed fabrication of finer detail tissue fabrication may also be possible in the future. This chapter introduced several methods used to deposit cells on arbitrary pattern and explored on the challenge in the implementation of inkjet printing technology to deposit cells and single cells on arbitrary pattern. This chapter also argued several adjustments and limitations for relatively large particles and cell printing, which are in a different size scale compared to ink particles in the commercial inkjet printer.

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Correspondence to Keisuke Morishima .

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The, R., Yamaguchi, S., Ueno, A., Akiyama, Y., Morishima, K. (2014). Rapid Single Cell Printing by Piezoelectric Inkjet Printer. In: Akashi, M., Akagi, T., Matsusaki, M. (eds) Engineered Cell Manipulation for Biomedical Application. Nanomedicine and Nanotoxicology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55139-3_3

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