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Fifty Years of Cytochrome P450 Research: Examples of What We Know and Do Not Know

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Fifty Years of Cytochrome P450 Research

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Abstract

The discovery of a new hemoprotein from rabbit liver in 1962 is one of the key moments in biology. Today, cytochrome P450 enzymes constitute a protein superfamily found in all domains of life and have even been described in some viruses. Herein, we describe some of the early experimental groundwork including P450 multiplicity and activity, electron transfer , and cellular localization that led to our early understanding of the molecular properties of P450 enzymes. Subsequently, following the advent of recombinant DNA technology and the development of heterologous expression systems and genomic sequencing, the traditional understanding of what actually defined a P450 was challenged: these included P450s with unusual molecular properties such as differences in electron partner proteins, fusion proteins, posttranslational modification(s) and unique catalytic activities. Selected examples from our own research findings are highlighted.

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Authors and Affiliations

  1. Institute of Life Science, Medical School, Swansea University, Singleton Park, Swansea, SA2 8PP, UK

    David C. Lamb

  2. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, 37232-0146, USA

    Michael R. Waterman

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  1. David C. Lamb
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  2. Michael R. Waterman
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Correspondence to Michael R. Waterman .

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  1. Showa Pharmaceutical University, Tokyo, Japan

    Hiroshi Yamazaki

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Lamb, D.C., Waterman, M.R. (2014). Fifty Years of Cytochrome P450 Research: Examples of What We Know and Do Not Know. In: Yamazaki, H. (eds) Fifty Years of Cytochrome P450 Research. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54992-5_3

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