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Homogeneous Glycoproteins: Overview

Glycoscience: Biology and Medicine

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Abstract

The heterogeneous nature of glycoproteins makes it difficult to understand how glycosylation affects the structure and function of glycoproteins. Development of new methods to prepare homogeneous glycoproteins with well-defined glycan structures is thus important to tackle this issue. This chapter describes various methods available for the synthesis of homogeneous glycoproteins for use to study the effect of glycan in glycoprotein folding, stabilization, and function, and to develop single glycoforms with optimal activity as pharmaceuticals. A major problem in the field is the lack of methods for modification of a specific glycan and a specific monosaccharide on a glycoprotein with multiple glycans, and this unsolved problem remains to be a major challenge in glycoscience.

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Authors and Affiliations

  1. The Scripps Research Institute, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, Taiwan

    Chi-Huey Wong

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  1. Chi-Huey Wong
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Correspondence to Chi-Huey Wong .

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Editors and Affiliations

  1. Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan

    Tamao Endo

  2. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Peter H. Seeberger

  3. Dept. Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Gerald W. Hart

  4. Academia Sinica, Nankang, Taipei, Taiwan

    Chi-Huey Wong

  5. Systems Glycobiology Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan

    Naoyuki Taniguchi

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© 2014 Springer Japan

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Wong, CH. (2014). Homogeneous Glycoproteins: Overview. In: Endo, T., Seeberger, P., Hart, G., Wong, CH., Taniguchi, N. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54836-2_50-1

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  • DOI: https://doi.org/10.1007/978-4-431-54836-2_50-1

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  • Publisher Name: Springer, Tokyo

  • Online ISBN: 978-4-431-54836-2

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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Chapter history

  1. Latest

    Homogeneous Glycoproteins, Overview
    Published:
    01 August 2014

    DOI: https://doi.org/10.1007/978-4-431-54836-2_50-2

  2. Original

    Homogeneous Glycoproteins: Overview
    Published:
    24 May 2014

    DOI: https://doi.org/10.1007/978-4-431-54836-2_50-1

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