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Chromatographische Trennmethoden für Peptide und Proteine

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Bioanalytik

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Zusammenfassung

Die Hochleistungsflüssigkeitschromatographie (HPLC) wurde in den letzten 30 Jahren zur unverzichtbaren Methode für die Trennung, Aufreinigung und Charakterisierung von synthetischen und biologischen Molekülen. Trotz großer Fortschritte, vor allem in der Instrumentierung, gibt es noch beachtliche Herausforderungen an die HPLC, wenn sie zur Analyse von biologischen Molekülen wie Peptiden und Proteinen in sehr komplexen Mischungen dienen soll, wie es z. B. in den Anwendungsbereichen Peptidomics, Proteomics und Degradomics der Fall ist.

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Author information

Authors and Affiliations

  1. School of Chemistry, Monash University, Melbourne, Australia

    Reinhard Boysen

Authors
  1. Reinhard Boysen
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Correspondence to Reinhard Boysen .

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Editors and Affiliations

  1. Institut für Biotechnologie, Technische Universität Berlin, Berlin, Deutschland

    Jens Kurreck

  2. Institut für Organische Chemie, Goethe Universität Frankfurt, Frankfurt am Main, Deutschland

    Joachim W. Engels

  3. Max Planck Institut für Biochemie, Planegg-Martinsried, Deutschland

    Friedrich Lottspeich

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Boysen, R. (2022). Chromatographische Trennmethoden für Peptide und Proteine. In: Kurreck, J., Engels, J.W., Lottspeich, F. (eds) Bioanalytik. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-61707-6_11

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