Abstract
Fluorescence in situ hybridization (FISH) provides an effective means to delineate chromosomes and their subregions during all stages of the cell cycle. This makes FISH particularly useful for studying chromosome behavior in species with minute genomes and/or weak chromosome condensation at metaphase, which is the case for model organisms such as the budding yeast Saccharomyces cerevisiae or Schizosaccharomyces pombe. Since its introduction in 1992, yeast FISH with composite whole chromosome or locus-specific probes in combination with immunofluorescence staining has become an indispensable tool in the analysis of chromosome behavior in metaphase and interphase cells, and especially of meiotic chromosome pairing of wild-type and mutant yeast strains.
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Acknowledgments
I thank J. Loidl, E. Trelles-Sticken, and A. Lorenz for stimulating discussions. The work in the lab of HS was partly supported by the DFG.
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Scherthan, H. (2017). Yeast Chromosome Dynamics Revealed by Immuno FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_50
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DOI: https://doi.org/10.1007/978-3-662-52959-1_50
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