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Optimization of a Whey Containing Medium for β-Galactosidase Production by Lactobacillus reuteri

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Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 333))

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Abstract

Lactobacillus reuteri MF2-2 was screened out for producing a high level of β-galactosidase from chicken fecal samples and identified with 16S rRNA gene sequence analysis. To reduce production cost, a new whey containing medium was formulated on the basis of MRS medium and optimized. Nutrients including whey, yeast extract, NH4H2PO4, and vitamin B1 were investigated at the individual and interactive level by the Taguchi orthogonal tests. The production of β-galactosidase was improved from 490.0 U/g in lactose-based MRS (L-MRS) medium to 814.6 U/g in the optimized whey-based MRS (W-MRS) medium. W-MRS medium did not comprise of lactose, peptone, and beef extract and was expected to cut the raw material cost approximately in half compared to L-MRS medium.

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Acknowledgments

This work was partly supported by the Tianjin Key Technology R&D Program (Grant No. 13ZCZDNC00600).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China

    Mengfei Li, Huiming Zhu, Huibin Qin & Hongjiang Yang

  2. Tianjin Shuang Lian Ke Xin Biotechnology Co., Ltd, Tianjin, China

    Yan Zhang

Authors
  1. Mengfei Li
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  2. Huiming Zhu
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  3. Huibin Qin
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  4. Yan Zhang
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  5. Hongjiang Yang
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Corresponding author

Correspondence to Hongjiang Yang .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Li, M., Zhu, H., Qin, H., Zhang, Y., Yang, H. (2015). Optimization of a Whey Containing Medium for β-Galactosidase Production by Lactobacillus reuteri . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_62

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