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Cell-Specific Aptamers for Disease Profiling and Cell Sorting

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Aptamers Selected by Cell-SELEX for Theranostics

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Abstract

The molecular recognition of medically relevant cell-surface proteins and other biomarkers by molecular probes plays a major role in this current era of molecular medicine. Molecular probes have served as platforms for diagnosis, prognostic indication and targeted radio- or chemotherapy in cancer medicine. Since cancer is generally a heterogeneous disease, the elucidation of new disease specific molecular features will facilitate our understanding of cancer. The development of new molecular probes to detect disease specific features will improve our ability to specifically target and treat cancers. Cell-specific aptamers have emerged as unique candidates for molecular identification of cancer cells. Single runs of cell-SELEX can generate panels of aptamers that target disease specific molecular markers with high affinity and selectivity. We have shown that these panels can be used for molecular profiling of cancer and aid in the diagnosis of cancer. The ability to detect diseased cells in biological fluids is important for early detection, monitoring disease progression or remission, and tracking drug efficacy. Our research has shown that aptamers can be used to purify cells from a flowing suspension of biological fluid. When integrated into microfluidic devices, aptamers can be used for enrichment of rare tumor cells and multiplexed cell sorting of heterogeneous cell mixtures. For these reasons, aptamers have emerged as unique candidates for molecular recognition and cell-isolation and their future contributions will be a key factor in molecular medicine.

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Author information

Authors and Affiliations

  1. Roche Molecular Systems, 700, Nickerson Road, Marlborough, MA, 01752, USA

    Kwame Sefah & Joseph Phillips

  2. Departments of Chemistry, Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, 32611, USA

    Cuichen Wu

Authors
  1. Kwame Sefah
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  2. Joseph Phillips
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  3. Cuichen Wu
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Correspondence to Kwame Sefah .

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Editors and Affiliations

  1. Department of Chemistry, Hunan University, Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering, Changsha, China & University of Florida, Gainesville, Florida, USA

    Weihong Tan

  2. Institute of Chemistry, Chinese Academy of Sciences, Beijing, China

    Xiaohong Fang

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Sefah, K., Phillips, J., Wu, C. (2015). Cell-Specific Aptamers for Disease Profiling and Cell Sorting. In: Tan, W., Fang, X. (eds) Aptamers Selected by Cell-SELEX for Theranostics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46226-3_9

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