Abstract
Peanut is a good source of dietary protein. Raising protein content in peanut will not only fill the growing need for vegetable protein, but also in most cases lower oil content, which is good news to health-conscious populations. However, no attempt has been made to isolate genes related to protein content in peanut. In the present study, a total of 40 unique differentially expressed genes in developing seeds of high-protein peanut mutant (SDPM) and its normal-protein wild type (SDPW) at 46 or 49 days after flowering were isolated using Genefishing technology. Of them, 8 sequences were undescribed previously; the rest 32 were found to be significantly similar to the sequences in GenBank nr database. Three genes potentially related to protein content in peanut, viz., P2-2-2, P2-92-2 and P1-89-1-5 with high homology to thioredoxin h, arachin ahy-4 and abc transporter, respectively, were selected for further analysis. All the 3 genes validated by qRT-PCR showed differential expression between SDPM and SDPW, with relative expression ranging from 0.41–10.60. The detailed functions of the differentially expressed genes isolated from developing seeds in the present study in conditioning peanut seed protein content are yet to be validated by transgenic experiments.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Yu SL, Wang CT, Yang QL et al (2011) Peanut genetics and breeding in China. Shanghai Science and Technology Press, Shanghai, pp 18–24
Zhang XY, Han SY, Xu J et al (2011) Genetic analysis of protein using major gene plus polygene methods in peanut (Arachis hypogaea L.). Chinese J Oil Crop Sci 33(2):118–122
Liang X, Zhou G, Hong X et al (2009) Overview of research progress on peanut (Arachis hypogaea L.) host resistance to aflatoxin contamination and genomics at the guangdong academy of agricultural sciences. Peanut Sci. 36:29–34
Sarvamangala C, Gowda MVC, Varshney RK (2011) Identification of quantitative trait loci for protein content, oil content and oil quality for groundnut (Arachis hypogaea L.). Field Crops Res 122:49–59
Wang CT, Zhang JC, Tang YY et al (2013) Peanut genetic improvement. Shanghai Science and Technology Press, Shanghai, p 531
Wang CT, Tang YY, Wang XZ et al (2011) Evaluation of groundnut genotypes from China for nutritional quality. J SAT Agric. Res. vol. 9. Available at: http://ejournal.icrisat.org/Volume9/Groundnut/Evaluation.pdf. (Accessed 31 May 2013)
Wan SB (ed) (2003) China peanut cultivation science. Shanghai Science & Technology Press, Shanghai pp 408–430
Tang YY, Wang CT, Yang GP et al (2011) Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.). Electronic J. Biotechnol. 14(5). DOI: 10.2225/vol14-issue5-fulltext-5
Weber H, Sreenivasulu N, Weschke W (2010) Molecular physiology of seed maturation and seed storage protein biosynthesis. In: Pua EC, Davey MR (eds) Plant developmental biology–biotechnological perspectives, Springer, Berlin Heidelberg, pp 83–104
Rolletschek H, Borisjuk L, Radchuk R et al (2004) Seed-specific expression of a bacterial phosphoenolpyruvate carboxylase in Vicia narbonensis increases protein content and improves carbon economy. Plant Biotechnol J 2(3):211–219
Rolletschek H, Hosein F, Miranda M et al (2005) Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins. Plant Physiol 137(4):1236–1249
Acknowledgments
We wish to express our sincere thanks to the financial support from China Agricultural Research System (CARS-14), Liaoning Natural Science Foundation Project for Fostering Talent (2014027029), Key Project of Liaoning Provincial Science and Technology Department (2011201021) and China National Science and Technology Support Project (2012BAD36B00).
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Yu, S.T. et al. (2015). Isolation of Differentially Expressed Genes from Developing Seeds of a High-Protein Peanut Mutant and Its Wild Type Using GenefishingTM Technology. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_5
Download citation
DOI: https://doi.org/10.1007/978-3-662-45657-6_5
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-45656-9
Online ISBN: 978-3-662-45657-6
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)