Abstract
Inulinases are classified among hydrolases, target the β-(2,1)-linkage of inulin and can be used to produce high-fructose corn syrup, fructo-oligosaccharides and alcohol, conferring upon inulinases significant potential for application in the food industry. A total of 28 strains that are capable of producing inulinase were isolated from the rhizosphere soil of Jerusalem artichoke (Helianthus tuberosus L.). Of these strains, the bacterial strain ni-3 was isolated through further screening. Based on its morphological, physiological and biochemical characteristics, ni-3 was identified by 16S rDNA sequences and systematic analysis. The 16S rDNA sequences of this strain had 99.5 % similarity with the partial sequence of Brevibacillus centrosporus, denominated as B. centrosporus ZF-9. The optimum conditions of fermentation were investigated. The optimum conditions for inulinase were as follows: inulin 45 g/L, yeast extract 11 g/L, and Na2HPO4 20 g/L. Under these conditions, the inulinase activity of B. centrosporus ZF-9 reached 6.41 U/mL.
Yang Zhang and Hongyang Zhu had equal contributions to this work.
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Acknowledgments
This work was supported by the Natural Science Foundation of Fujian Province (No. 2012J05015), Educational Commission of Fujian Province (JA12430), and Doctoral Research Startup Foundation of Liaocheng University (No. 31805).
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Zhang, Y., Zhu, H., Wang, J., Zhou, X., Xu, W., Shi, H. (2015). Isolation and Identification of an Inulinase-Producing Strain and the Optimization of Its Fermentation Condition. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_11
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DOI: https://doi.org/10.1007/978-3-662-45657-6_11
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