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Imaging Techniques for Corneal Disorders

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Advances in Medical and Surgical Cornea

Part of the book series: Essentials in Ophthalmology ((ESSENTIALS))

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Abstract

Corneal imaging has dramatically increased our abilities to effectively evaluate and treat patients with complex corneal disorders that, in decades past, were very difficult to manage without direct ex vivo evaluation, whether by culture or by pathologic evaluation. Even now, confocal microscopy, anterior segment optical coherence tomography, and specular microscopy allow us to evaluate normal anatomy and postsurgical results while also allowing the evaluation of pathologic conditions such as atypical infectious keratitis, corneal dystrophies, and postsurgical complications.

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References

  • Asrani S, Sarunic M, Santiago C, Izatt J. Detailed visualization of the anterior segment using fourier-domain optical coherence tomography. Arch Ophthalmol. 2008;126(6):765–71.

    Article  PubMed Central  PubMed  Google Scholar 

  • Bourne WM, Kaufman HE. Specular microscopy of human corneal endothelium in vivo. Am J Ophthalmol. 1976;81(3):319–23.

    Article  CAS  PubMed  Google Scholar 

  • Brooks AM, Grant G, Gillies WE. Differentiation of posterior polymorphous dystrophy from other posterior corneal opacities by specular microscopy. Ophthalmology. 1989;96(11):1639–45.

    Article  CAS  PubMed  Google Scholar 

  • Cavanagh HD, Petroll WM, Alizadeh H, He YG, McCulley JP, Jester JV. Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease. Ophthalmology. 1993;100(10):1444–54.

    Article  CAS  PubMed  Google Scholar 

  • Cavanaugh HD, Jester JV, Essepian J, Shields W, Lemp MA. Confocal microscopy of the living eye. CLAO J. 1990;16(1):65–73.

    Google Scholar 

  • Cavanaugh HD, Petroll WM, Jester JV. The application of confocal microscopy to the study of living systems. Neurosci Biobehav Rev. 1993;17(4):483–98.

    Article  Google Scholar 

  • Chang PY, Carrel H, Huang JS, Wang IJ, Chen WL, Wang JY, Hu FR. Decreased density of corneal basal epithelium and subbasal corneal nerve bundle changes in patients with diabetic retinopathy. Am J Ophthalmol. 2006;142(3):488–90.

    Article  PubMed  Google Scholar 

  • Chen MC, Cortes DE, Harocopos G, Mannis MJ. Epithelial downgrowth after penetrating keratoplasty: imaging by high-resolution optical coherence tomography and in vivo confocal microscopy. Cornea. 2013;32(11):1505–8.

    Article  PubMed  Google Scholar 

  • Chiou AG, Kaufman SC, Beuerman RW, Ohta T, Soliman H, Kaufman HE. Confocal microscopy in corneal guttata and Fuchs’ endothelial dystrophy. Br J Ophthalmol. 1999a;83(2):185–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chiou AG, Kaufman SC, Beuerman RW, Ohta T, Yaylali V, Kaufman HE. Confocal microscopy in the iridocorneal endothelial syndrome. Br J Ophthalmol. 1999b;83(6):697–702.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Dhaliwal JS, Kaufman SC, Chiou AGY. Current applications of clinical confocal microscopy. Curr Opin Ophthalmol. 2007;18(4):300–7.

    Article  PubMed  Google Scholar 

  • Díaz-Valle D, Benítez del Castillo Sánchez JM, Castillo A, Sayagués O, Moriche M. Endothelial damage with cataract surgery techniques. J Cataract Refract Surg. 1998;24(7):951–5.

    Article  PubMed  Google Scholar 

  • Edelhauser HF. The resiliency of the corneal endothelium to refractive and intraocular surgery. Cornea. 2000;19(3):263–73.

    Article  CAS  PubMed  Google Scholar 

  • Erie JC. Corneal wound healing after photorefractive keratectomy: a 3-year confocal microscopy study. Trans Am Ophthalmol Soc. 2003;101:293–333.

    PubMed Central  PubMed  Google Scholar 

  • Erie JC, McLaren JW, Hodge DO, Bourne WM. Recovery of corneal subbasal nerve density after PRK and LASIK. Am J Ophthalmol. 2005;140(6):1059–64.

    Article  PubMed  Google Scholar 

  • Eye Bank Association of America. 2013 Eye Banking Statistical Report. 2008. http://www.restoresight.org/wp-content/uploads/2013/04/2012_Statistical_Report_FINAL-reduced-size-4-10.pdf

  • Glasser DB, Matsuda M, Gager WE, Edelhauser HF. Corneal endothelial morphology after anterior chamber lens implantation. Arch Ophthalmol. 1985;103(9):1347–9.

    Article  CAS  PubMed  Google Scholar 

  • Huang D, Swanson EA, Lin CP, et al. Optical coherence tomography. Science. 1991;254:1178–81.

    Article  CAS  PubMed  Google Scholar 

  • Izatt JA, Hee MR, Swanson EA, et al. Micrometer-scale resolution imaging of the anterior eye in vivo with optical coherence tomography. Arch Ophthalmol. 1994;112:1584–9.

    Article  CAS  PubMed  Google Scholar 

  • Kaufman SC, Kaufman HE. How has confocal microscopy helped us in refractive surgery? Curr Opin Ophthalmol. 2006;17:380–8.

    Article  PubMed  Google Scholar 

  • Kaufman SC, Beuerman RW, Kaufman HE. Diagnosis of advanced Fuchs’ endothelial dystrophy with the confocal microscope. Am J Ophthalmol. 1993;116(5):652–3.

    Article  CAS  PubMed  Google Scholar 

  • Kaufman SC, Laird JA, Cooper R, Beuerman RW. Diagnosis of bacterial contact lens related keratitis with the white-light confocal microscope. CLAO J. 1996;22(4):274–7.

    CAS  PubMed  Google Scholar 

  • Kitzmann AS, Winter EJ, Nau CB, McLaren JW, Hodge DO, Bourne WM. Comparison of corneal endothelial cell images from a noncontact specular microscope and a scanning confocal microscope. Cornea. 2005;24(8):980–4.

    Article  PubMed  Google Scholar 

  • Kobayashi A, Sakurai M, Shirao Y, Sugiyama K, Ohta T, Amaya-Ohkura Y. In vivo confocal microscopy and genotyping of a family with Thiel-Behnke (honeycomb) corneal dystrophy. Arch Ophthalmol. 2003;121(10):1498–9.

    Article  PubMed  Google Scholar 

  • Kobayashi A, Fujiki K, Murakami A, Sugiyama K. In vivo laser confocal microscopy findings and mutational analysis for Schnyder’s crystalline corneal dystrophy. Ophthalmology. 2009;116(6):1029–37.

    Article  PubMed  Google Scholar 

  • Koester C. Scanning mirror microscope with optical sectioning characteristics: applications in ophthalmology. Appl Opt. 1980;19:1749–57.

    Google Scholar 

  • Laing RA, Leibowitz HM, Oak SS, Chang R, Berrospi AR, Theodore J. Endothelial mosaic in Fuchs’ dystrophy. A qualitative evaluation with the specular microscope. Arch Ophthalmol. 1981;99(1):80–3.

    Article  CAS  PubMed  Google Scholar 

  • Lass JH, Sugar A, Benetz BA, et al. Endothelial cell density to predict endothelial graft failure after penetrating keratoplasty. Arch Ophthalmol. 2010;128(1):63–9.

    Article  PubMed Central  PubMed  Google Scholar 

  • Lemp MA, Dilly PN, Boyde A. Tandem-scanning (confocal) microscopy of the full thickness cornea. Cornea. 1985–1986;4(4):205–9.

    Google Scholar 

  • Mathers WD, Sutphin JE, Folberg R, et al. Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol. 2000;118:178–83.

    Article  CAS  PubMed  Google Scholar 

  • Maurice DM. Cellular membrane activity in the corneal endothelium of the intact eye. Experientia. 1968;24(11):1094–5.

    Article  CAS  PubMed  Google Scholar 

  • Mazzotta C, Caragiuli S, Caporossi A. Confocal microscopy in a case of crystalline keratopathy in a patient with smouldering multiple myeloma. Int Ophthalmol. 2014;34(3):651–4.

    Article  PubMed  Google Scholar 

  • McVeigh K, Cornish KS, Reddy AR, Vakros G. Retained Descemet’s membrane following penetrating keratoplasty for Fuchs’ endothelial dystrophy: a case report of a post-operative complication. Clin Ophthalmol. 2013;7:1511–4.

    PubMed Central  PubMed  Google Scholar 

  • Minsky M. Memoir on inventing the confocal microscope. Scanning. 1988;10:128–38.

    Article  Google Scholar 

  • Mishima S. Clinical investigations on the corneal endothelium. Ophthalmology. 1982;89(6):525–30.

    Article  CAS  PubMed  Google Scholar 

  • Moller-Pederson T, Cavanaugh HD, Petroll WM, Jester JV. Stromal wound healing explains refractive instability and haze development after photorefractive keratectomy: a 1-year confocal microscopic study. Ophthalmology. 2000;107(7):1235–45.

    Article  Google Scholar 

  • Naroo SA, Cervino A. Corneal topography and its role in refractive surgery. In: Narco SA, Butterworth H, editors. Refractive surgery: a guide to assessment and management. London: Elsevier; 2004. p. 9–16.

    Chapter  Google Scholar 

  • Niederer R, Perumal D, Sherwin T, et al. Corneal innervation and cellular changes after corneal transplantation: an in vivo confocal microscopy study. Invest Ophthalmol Vis Sci. 2007;48:621–6.

    Article  PubMed  Google Scholar 

  • Patel DV, Sherwin T, McGhee CNJ. Laser scanning in vivo confocal microscopy of the normal human corneoscleral limbus. Invest Ophthalmol Vis Sci. 2006;47(7):2823–7.

    Article  PubMed  Google Scholar 

  • Petran M, Hadravsky M, Egger MD, Galambos R. Tandem-scanning reflected-light microscope. J Opt Soc Am. 1968;58:661–4.

    Article  Google Scholar 

  • Petran M, Hadravsky M, Benes J, Boyde A. In vivo microscopy using the tandem scanning microscope. Ann N Y Acad Sci. 1986;483:440–7.

    Article  CAS  PubMed  Google Scholar 

  • Petroll WM, Cavanaugh HD, Jester JV. Three-dimensional imaging of corneal cells using in vivo confocal microscopy. J Microsc. 1993;170(Pt 3):213–9.

    Article  CAS  PubMed  Google Scholar 

  • Pfister DR, Cameron JD, Krachmer JH, Holland EJ. Confocal microscopy findings of Acanthamoeba keratitis. Am J Ophthalmol. 1997;115:714–8.

    Google Scholar 

  • Pichierri P, Martone G, Loffredo A, Traversi C, Polito E. In vivo confocal microscopy in a patient with conjunctival lymphoma. Clin Experiment Ophthalmol. 2008;36(1):67–9.

    Article  PubMed  Google Scholar 

  • Price Jr FW, Price MO. Does endothelial cell survival differ between DSEK and standard PK? Ophthalmology. 2009;116(3):367–8.

    Article  PubMed  Google Scholar 

  • Prydal JI, Franc F, Dilly PN, Kerr Muir MG, Corbett MC, Marshall J. Keratocyte density and size in conscious humans by digital image analysis of confocal images. Eye (Lond). 1998;12(pt 3a):337–42.

    Article  Google Scholar 

  • Radhakrishnan S, Rollins AM, Roth JE, et al. Real-time optical coherence tomography of the anterior segment at 1310 nm. Arch Ophthalmol. 2001;119:1179–85.

    Article  CAS  PubMed  Google Scholar 

  • Rosenberg ME, Tervo TM, Petroll WM, Vesaluoma MH. In vivo confocal microscopy of patients with corneal recurrent erosion syndrome or epithelial basement membrane dystrophy. Ophthalmology. 2000;107(3):565–73.

    Article  CAS  PubMed  Google Scholar 

  • Rosenberg ME, Terno TM, Muller LJ, Moilanen JA, Vesaluoma MH. In vivo confocal microscopy after herpes keratitis. Cornea. 2002;21(3):265–9.

    Article  PubMed  Google Scholar 

  • Schultz RO, Matsuda M, Yee RW, Edelhauser HF, Schultz KJ. Corneal endothelial changes in type I and type II diabetes mellitus. Am J Ophthalmol. 1984;98(4):401–10.

    Article  CAS  PubMed  Google Scholar 

  • Sherrard ES, Novakovic P, Speedwell L. Age-related changes of the corneal endothelium and stroma as seen in vivo by specular microscopy. Eye (Lond). 1987;1(Pt 2):197–203.

    Article  Google Scholar 

  • Sherrard ES, Frangoulis MA, Muir MG. On the morphology of cells of posterior cornea in the iridocorneal endothelial syndrome. Cornea. 1991;10(3):233–43.

    Article  CAS  PubMed  Google Scholar 

  • Steinert R, Huang D. Anterior segment optical coherence tomography. 1st ed. Thorofare: Slack Inc.; 2008.

    Google Scholar 

  • Vaddavalli PK, Garg P, Sharma S, Thomas R, Rao GN. Confocal microscopy for Nocardia keratitis. Ophthalmology. 2006;113(9):1645–50.

    Article  PubMed  Google Scholar 

  • Werner LP, Werner L, Dighiero P, Legeais JM, Renard G. Confocal microscopy in Bowman and stromal corneal dystrophies. Ophthalmology. 1999;106(9):1697–704.

    Article  CAS  PubMed  Google Scholar 

  • Winchester K, Mathers WD, Sutphin JE. Diagnosis of Aspergillus keratitis in vivo with confocal microscopy. Cornea. 1997;16:27–31.

    Article  CAS  PubMed  Google Scholar 

  • Wylegala E, Teper S, Nowinska AK, Milka M, Dobrowolski D. Anterior segment imaging: Fourier-domain optical coherence tomography versus time-domain optical coherence tomography. J Cataract Refract Surg. 2009;35(8):1410–4.

    Article  PubMed  Google Scholar 

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Conflict of Interest

Alla Kelly, MD; Stephen C. Kaufman, MD, PhD; Jonathan Lass MD; Denice Barsness, CRA, COMT, ROUB, CDOS, FOPS; Beth Ann Benetz, CRA, FOPS; and Pankaj Gupta, MD, MS declare that they have no conflict of interest related to the subject matter in the chapter.

Informed Consent

(2) No human studies were carried out by the authors for this article.

Animal Studies

(2) No animal studies were carried out by the authors for this article.

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Authors and Affiliations

  1. Department of Ophthalmology, University of Minnesota, Minneapolis, MN, USA

    Alla Kelly MD & Stephen C. Kaufman MD, PhD

  2. Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH, USA

    Jonathan Lass MD, Beth Ann Benetz CRA, FOPS & Pankaj Gupta MD, MS

  3. Department of Ophthalmology Ophthalmic Diagnostic Center, California Pacific Medical Center, San Francisco, CA, USA

    Denice Barsness CRA, COMT, ROUB, CDOS, FOPS

Authors
  1. Alla Kelly MD
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  2. Stephen C. Kaufman MD, PhD
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  3. Jonathan Lass MD
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  4. Denice Barsness CRA, COMT, ROUB, CDOS, FOPS
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  5. Beth Ann Benetz CRA, FOPS
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  6. Pankaj Gupta MD, MS
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Corresponding author

Correspondence to Pankaj Gupta MD, MS .

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Editors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA

    Bennie H. Jeng

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Kelly, A., Kaufman, S.C., Lass, J., Barsness, D., Benetz, B.A., Gupta, P. (2014). Imaging Techniques for Corneal Disorders. In: Jeng, B. (eds) Advances in Medical and Surgical Cornea. Essentials in Ophthalmology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-44888-5_4

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  • DOI: https://doi.org/10.1007/978-3-662-44888-5_4

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