Abstract
The ability to sense and measure adhesion forces by using force spectroscopy techniques has opened new perspectives in the field of mechanobiology. Single-cell force spectroscopy enables to directly measure interactive forces of single living cell with extracellular environment (i.e., cell, proteins and tissue) with extremely high resolution (single-protein level). Cell adhesion processes rely on the interaction of adhesion proteins with their environment. Cells sense and recognize the specific forces that are generated by the interaction with the environment, and transduce them into biochemical signals by which the cells evolve, move and grow. Single-cell force spectroscopy is the ideal tool to measure these forces and investigate the cellular response from its origin.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Müller DJ and Dufrêne YF (2011) Atomic force microscopy: a nanoscopic window on the cell surface. Trends Cell Biol 21:461–469
Kondra S, Laishram J, Ban J, Migliorini E, Di Foggia V, Lazzarino M, Torre V, Ruaro ME (2009) Integration of confocal and atomnic force microscopy images. J Neuroscience Meth 177:94–107
Ban J, Migliorini E, Di Foggia V, Lazzarino M, Ruaro ME, Torre V (2011) Fragmentation as a mechanism for growth cone pruning and degeneration. Stem Cells Dev 20:1031–1041
Lasalvia M, Perna G, Mezzenga E, Migliorini E, Lazzarino M, L'Abbate N, Capozzi V (2011) Atomic force microscopy investigation of morphological changes in living keratinocytes treated with HgCl2 at not cytotoxic doses. J Microscopy 243:40–46
Polano M, Bek A, Benetti F, Lazzarino M, Legname G (2009) Structural insights into alternate aggregated prion protein forms source. J Mol Biol 393:1033–1042
Müller DJ, Janovjak H, Lehto T, Kuerschner L, Anderson K (2002) Observing structure, function and assembly of single proteins by AFM. Prog Biophys Mol Biol 79:1–43
Nordin D, Donlon L, Frankel D (2012) Characterising single fibronectin-integrin complexes. Soft Matter 8:6151–6160
Müller DJ, Helenius J, Alsteens D, Dufrêne YF (2009) Force probing surfaces of living cells to molecular resolution. Nat Chem Biol 5:383–390
Raman PS, Alves CS, Wirtz D, Konstantopoulos K (2011) Single-molecule binding of CD44 to fibrin versus P-selectin predicts their distinct shear-dependent interactions in cancer. J Cell Sci 124:1903–1910
Raab A, Han W, Badt D, Smith-Gill S-J, Lindsay SM, Schindler H, Hinterdorfer P (1999) Antibody recognition imaging by force microscopy. Nat Biotechnol 17:901–905
Hinterdorfer P and Dufrêne YF (2006) Detection and localization of single molecular recognition events using atomic force microscopy. Nat Methods 3:347–355
Puech P-H, Poole K, Knebel D, Müller DJ (2006) A new technical approach to quantify cell-cell adhesion forces by AFM. Ultramicroscopy 106:637–644
Helenius J, Heisenberg C-P, Gaub HE, Müller DJ (2008) Single-cell force spectroscopy. J Cell Sci 121:1785–1791
Friedrichs J, Legate KR, Schubert R, Bharadwaj M, Werner C, Müller DJ, Benoit M (2013) A practical guide to quantify cell adhesion using single-cell force spectroscopy. Methods 60:169–178
Manzanares M-V, Webb DJ, Horwitz AR (2005) Cell migration at a glance. J Cell Sci 118:4917–4919F
Hynes RO (1992) Integrins: Versatility, modulation and signaling in cell adhesion. Cell 69:11–25
Huttenlocher A and Horwitz AR (2011) Integrings in cell migration. Cold Spring Harb Perspect Biol 3:a0055074
Campbell ID and Humphries MJ (2011) Integrin structure, activation and interactions. Cold Spring Harb Perspect Biol 3:a004994
GarcÃa AJ and gallant ND (2003) "Stick and Grip": measurement system and quantitative analyses of integrin-mediated cell adhesion strength. Cell Biochem Biophys 39:61–74
Garcia AJ, Ducheyne P, Boettinger D (1997) Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials. Biomaterials 18:1091–1098
Gallant ND, Michael KE, Garcia AJ (2005) Cell Adhesion Strengthening: Contributions of Adhesive area, integrin binding and focal adhesion assembly. Mol Biol Cell 16:4329–4340
Owen GRH, Meredith DO, Gwynn I, Richards RG (2005) Focal adhesion quantification- A new assay of material biocompatibility? Review European Cells and Materials 9:85–96
Hills EC, Mustafa YYG, Bennett J, Siamantouras E, Liu K-K, Squires PE (2012) Calcium-sensing receptor activation increases cell-cell adhesion and β-cell function. Cell Physiol Biochem 30:575–586
Mack PJ, Kaazempur-Mofrad MR, Karcher H, Lee RT, Kamm RD (2004) Force-induced focal adhesion translocation: effects of force amplitude and frequency. Am J Physiol Cell Physiol 287:C954–C962
Estevez M, Fernandez-Ulibarri I, Martinez E, Egeac G and Samitiera J (2010) Changes in the internal organizzation of the cell by microstructured substrates Soft Matter 6:582–590
Bakker GJ, Eich C, Torreno-Pina JA, Diez-Ahedoa R, Perez-Samper G, Van Zanten TS, Figdor CG, Cambi A, and Garcia-Parajo MF (2012) Lateral mobility of individual integrin nanoclusters orchestrates the onset for leukocyte adhesion. PNAS 109:4869–4874
Franz CM and Müller DJ (2005) Analysing focal adhesion structure by atomic force microscopy. J Cell Sci 118:5315–5323
Dufrêne YF and Pelling AE (2013) Force nanoscopy of cell mechanics and cell adhesion. Nanoscale 5:4094–4104
Baumgartner W, Hinterdorfer P, Ness W, Raab, Vestweber D, Scindler H and Drenckhahn (2000) Cadherin interaction probed by atomic force microscopy. PNAS 97:4005–4010
Wojcikiewicz EP, Abdulreda MH, Zhang X, Moy VT (2006) Force spectroscopy of LFA-1 and its ligand ICAM-1 ICAM-2. Biomacromolecules 7:3188–3195
Provenzano PP and Keely PJ (2011) Mechanical signaling through the cytoskeleton regulates cell proliferation by coordinated focal adhesion and Rho GTPase signaling. J Cell Sci 124:1195–1205
Wirtz D, Konstantopoulos K, Searson PC (2011) The physics of cancer: the role of physical interactions and mechanical forces in metastasis. Nat Rev 11:512–522
Lama G, Papi M, Angelucci C, Maulucci G, Sica G, De Spirito M (2013) Leuprorelin acetate long-lasting effects on GnRH receptors of prasttae cancer cells: an atomic force microscopy study of agonist/receptor interaction. PlosOne 8:e52530
Puntheeranurak T, Neundlinger I, Kinne RKH, Hinterdorfer P (2011) Single molecule recognition force spectroscopy of trasmembrane transporters on living cells. Nat Protocols 6:1443–1452
Hutter J and Bechhoefer J (1993) Calibration of atomic force microscopy tips. Rev Sci Instrum 64:1868–1873
Panorchan P, Thompson MS, Davis KJ, Tseng Y, Konstantopoulos K, Wirtz D (2005) Single-molecule analysis of cadherin-mediated cell-cell adhesion. J Cell Sci 119:66–74
Hoffmann SC, Cohnen A, Ludwig T, Watzl C (2011) 2B4 engagement mediates rapid LFA-1 and actin-dependent NK cell adhesion to tumor cell as measured by single cell force spectroscopy. J Immunol 186:2757–2764
Lim TS, Hao Goh JK, Mortellaro A, Lim CT, Hämmerling GJ, Ricciardi-Castagnoli P (2012) CD80 and CD86 differentially regulate mechanical interactions of T-cells with antigen-presenting dendritic cells and B-cells. PlosOne 7:e45185
Evans  EA and Calderwood DA (2007) Forces and bond dynamics in cell adhesion. Sci 316:1148–1153
Sherer NM (2013) Long-distance relationships: do membrane nanotubes regulate cell-cell communication and disease progression? Mol Biol Cell 24:1095–1098
Evans E and Ritchie K (1997) Dynamic strength of molecular adhesion bonds. Biophys J 72:1541–1555
Davis DM and Sowinski S (2008) Membrane nanotubes dynamic long-distance connections between animal cells. Nat Rev Mol Cell Biol 9:431–436
Wojcikiewicz EP, Zhang X, Moy VT (2003) Force and compliance measurements on living cells using atomic force microscopy (AFM). Biol Proced Online 6:1–9
Tulla M, Helenius J, Jokinen J, Taubenberger A, Müller DJ, Heino J (2008) TPA primes α2β1 integrins for cell adhesion. FEBS letters 582:3520–3524
Benoit M, Gabriel D, Gerisch G and Gaub HE (2000) Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat Cell Biol 2:313–317
Hoffmann S, Hosseini BH, Hecker M, Louban I, Bulbuc N, Garbi N, Wabnitz GH, Samsag Y, Spatz JP, Hämmerling GJ (2011) Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells. Immunol Lett 136:13–20
Friedrichs J, Helenius J, Müller DJ (2010) Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk. Proteomics 10:1455–1462
Hills CE, Jin T, Siamantouras E, Liu I K-K, Jefferson KP, Squires PE (2013) "Special K" and a loss of cell-to-cell adhesion in proximal tubule-derived epithelial cells: modulation of the adherens junctions complex by ketamine. PlosOne 8:e71819
Taubenberger A, Cisneros DA, Friedrichs J, Puech P-H, Müller DJ, Franz CM (2007) Revealing early steps of 21 integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy. Mol Biol Cell 18:1634–1644
Yilmaz M and Christofori G (2010) Mechanism of motility in metastasizing cells. Mol Cancer Res 8:629–642
Sariisik E, Docheva D, Padula D, Popov C, Opfer J, Schieker M, Clausen-Schaumann H, Benoit M (2013) Probing the interaction forces of prostate cancer cells with collagen I and bone marrow derived stem cells on the single cell level. PlosOne 8:e57706
Taubenberger AV, Quent VM, Thibaudeau L, Clements JA, Hutmacher DW (2013) Delineating breast cancer cell interactions with engineered bone microenvironments. J Bone Miner Res 28:1399–1411
Canale C, Petrelli A, Salerno M, Diaspro A, Dante S (2013) A new quantitative experimental approach to investigate single cell adhesion on  multifunctional substrates. Biosens Bioelectron 48:172–179
Migliorini E, Ban J, Grenci G, Andolfi L, Pozzato A, Tormen M, Torre V, Lazzarino M (2013) Nanomechanics controls neuronal precursors adhesion and differentiation. Biotechnol Bioeng 110:2301–2310
Bertoncini P, Le Chevalier S, Lavenus S, Layrolle P and Louarn G (2012) Early adhesion of human mesenchymal stem cells on TiO2 surfaces studied by single-cell force spectroscopy measurements. J Mol Recogn 25:262–269
Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Müller DJ, Hyman AA (2011) Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding. Nat 469:226–230
Favre M, Polesel-Maris J, Overstolz T, Niedermann P, Dasen S, Gruener S, Ischer R, Vettinger P, Liley M, Heinzelmann H, Meister A (2011) Parallel AFM imaging and force spectroscopy using two-dimensional probe arrays for application in cell biology. J Mol Recogn 24:4446–4452
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Andolfi, L., Lazzarino, M. (2014). Investigating Adhesion Proteins by Single Cell Force Spectroscopy. In: Benfenati, F., Di Fabrizio, E., Torre, V. (eds) Novel Approaches for Single Molecule Activation and Detection. Advances in Atom and Single Molecule Machines. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-43367-6_8
Download citation
DOI: https://doi.org/10.1007/978-3-662-43367-6_8
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-43366-9
Online ISBN: 978-3-662-43367-6
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)