Abstract
The chromatographer in the laboratory is nowadays facing a wide variety of capillary columns with different polarities, lengths, inner diameters, and film thicknesses that are offered by numerous vendors. The selection of a proper column for a given separation problem and the optimization of the operating conditions can be challenging. This chapter starts with a discussion of the carrier gas, including thoughts for the selection of the carrier gas, gas purification, and operating conditions. Then, starting with the influence of the column parameters on retention and the separation, the importance of the phase ratio, column temperature, and elution temperature is explained. Finally, the development, optimization, and translation of GC methods are reviewed and degradation and rearrangement reactions in GC and their recognition are discussed.
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Engewald, W., Dettmer-Wilde, K. (2014). Selection of Capillary Columns and Operating Conditions. In: Dettmer-Wilde, K., Engewald, W. (eds) Practical Gas Chromatography. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54640-2_4
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DOI: https://doi.org/10.1007/978-3-642-54640-2_4
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