Abstract
During cultivation on n-alkanes as the sole source of carbon and energy, a bacterial isolate, ME102T, was obtained from a seawater/sediment sample collected in the harbor of Messina (Italy) from the depth of 8 m. Possessing a very narrow spectrum of consumable organic substrates almost exclusively restricted to long-chain aliphatic hydrocarbons, alkanoates and alkanoles, this Gram-negative, aerobic, motile, rod-shaped bacterium appeared to belong to the group of the so-called obligate marine hydrocarbonoclastic bacteria. The ME102T cells formed a biofilm on the surface of the alkane droplets and were likely adapted to the lifestyle on organic aggregates. Large-scale intracellular accumulation of C16:0 and C18:0 alkanoates (more than 50 % of the total cell mass) was observed at stationary phase of growth. 16S rRNA gene sequence analysis showed that ME102T isolate represents a distinct lineage in the order Oceanospirillales and had <92 % sequence identity to the closest members from families Hahellaceae and Oceanospirillaceae. On the basis of physiological, phenotypic, metabolic data and distant phylogenetic position, the hydrocarbonoclastic isolate ME102T was described as a novel species within a new genus, Oleiphilus messinensis gen. nov., sp. nov., in a new family, Oleiphilaceae fam. nov. Although many years have passed since the first isolation and valid publication, the family Oleiphilaceae and genus Oleiphilus are still represented by single species O. messinensis and the type strain ME102T remains as unique isolated bacterium.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Acosta-Gonzalez A, Rossello-Mora R, Marques S (2013) Characterization of the anaerobic microbial community in oil-polluted subtidal sediments: aromatic biodegradation potential after the Prestige oil spill. Environ Microbiol 15:77–92
Alcaide M, Tornes J, Stogios PJ, Xu X, Gertler C, Di Leo R, Bargiela R, Lafraya A, Guazzaroni M-E, Lopez-Cortes N, Chernikova TN, Golyshina OV, Nechitaylo TY, Plumeier I, Pieper DH, Yakimov MM, Savchenko A, Golyshin PN, Ferrer M (2013) Single residues dictate the co-evolution of dual esterases-MCP hydrolases from the alpha/beta hydrolase family. Biochem J 454:157–166
Camilli R, Reddy CM, Yoerger DR, Van Mooy BAS, Jakuba MV, Kinsey JC, McIntyre CP, Sylva SP, Maloney JV (2010) Tracking hydrocarbon plume transport and biodegradation at deepwater horizon. Science 330:201–204
Dyksterhouse SE, Gray JP, Herwig RP, Lara JC, Staley JT (1995) Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol 45:116–123
Golyshin PN, Chernikova TN, Abraham W-R, Lünsdorf H, Timmis KN, Yakimov MM (2002) Oleiphilaceae fam. nov., to include Oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. Int J Syst Evol Microbiol 52:901–911
Golyshin PN, Martins Dos Santos VA, Kaiser O, Ferrer M, Sabirova YS, Lunsdorf H, Chernikova TN, Golyshina OV, Yakimov MM, Puhler A, Timmis KN (2003) Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems. J Biotechnol 106:215–220
Golyshin PN, Werner J, Chernikova TN, Tran H, Ferrer M, Yakimov MM, Teeling H, Golyshina OV, MAMBA Scientific Consortium (2013) Genome sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T). Genome Announc 1(2):e00141-13. doi:10.1128/genomeA.00141-13
Harayama S, Kasai Y, Hara A (2004) Microbial communities in oil-contaminated seawater. Curr Opin Biotechnol 15:205–214
Hazen TC, Dubinsky EA, DeSantis TZ, Andersen GL, Piceno YM, Singh N, Jansson JK, Probst A, Borglin SE, Fortney JL, Stringfellow WT, Bill M, Conrad ME, Tom LM, Chavarria KL, Alusi TR, Lamendella R, Joyner DC, Spier C, Baelum J, Auer M, Zemla ML, Chakraborty R, Sonnenthal EL, D'haeseleer P, Holman HY, Osman S, Lu Z, Van Nostrand JD, Deng Y, Zhou J, Mason OU (2010) Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science 330:204–208
Head IM, Jones DM, Röling WFM (2006) Marine microorganisms make a meal of oil. Nat Rev Microbiol 4:173–182
Kube M, Chernikova TN, Al-Ramahi Y, Beloqui A, Lopez-Cortez N, Guazzaroni M-E, Heipieper HJ, Sven Klages K, Kotsyurbenko OR, Langer I, Nechitaylo TY, Lünsdorf H, Fernández M, Silvia Juárez S, Ciordia S, Singer A, Kagan O, Egorova O, Petit PA, Stogios P, Kim Y, Tchigvintsev A, Flick R, Denaro R, Genovese M, Albar JP, Reva ON, MartÃnez-Gomariz M, Tran H, Ferrer M, Savchenko A, Yakunin AF, Yakimov MM, Golyshina OV, Reinhardt R, Golyshin PN (2013) Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica. Nat Commun 4:a2156
Kuhn E, Bellicanta GS, Pellizari VH (2009) New alk genes detected in Antarctic marine sediments. Environ Microbiol 11:669–673
Lai Q, Li W, Wang B, Yu Z, Shao Z (2012) Complete genome sequence of the pyrene-degrading bacterium Cycloclasticus sp. strain P1. J Bacteriol 194:6677
Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF (2012) Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica. Biochem J 445:193–203
Mason OU, Hazen TC, Borglin S, Chain PS, Dubinsky EA, Fortney JL, Han J, Holman HY, Hultman J, Lamendella R, Mackelprang R, Malfatti S, Tom LM, Tringe SG, Woyke T, Zhou J, Rubin EM, Jansson JK (2012) Metagenome, metatranscriptome and single-cell sequencing reveal microbial response to deepwater horizon oil spill. ISME J 6:1715–1727
Paisse S, Goni-Urriza M, Coulon F, Duran R (2010) How a bacterial community originating from a contaminated coastal sediment responds to an oil input. Microb Ecol 60:394–405
Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Pühler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorhölter FJ, Weidner S, Kaiser O, Golyshin PN (2006) Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nat Biotechnol 24:997–1004
Yakimov MM, Golyshin PN, Lang S, Moore ER, Abraham W-R, Lunsdorf H, Timmis KN (1998) Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348
Yakimov MM, Timmis KN, Golyshin PN (2007) Obligate oil-degrading marine bacteria. Curr Opin Biotechnol 18:257–266
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer-Verlag Berlin Heidelberg
About this entry
Cite this entry
Yakimov, M.M., Golyshin, P.N. (2014). The Family Oleiphilaceae . In: Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (eds) The Prokaryotes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38922-1_285
Download citation
DOI: https://doi.org/10.1007/978-3-642-38922-1_285
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-38921-4
Online ISBN: 978-3-642-38922-1
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences