Abstract
Near-infrared (NIR) spectroscopy, a rapid and nondestructive analytical technique, was applied to predict the NH4 + concentration of the culture broth from temperature triggered glutamate fermentation. NIR data of 164 samples of supernatant were analyzed by partial least squares (PLS) regression with several spectra preprocessing methods. The coefficient of determination (R2), model root mean square error of calibration (RMSEC), and root-mean-square error of prediction (RMSEP) of the test calibration for NH4 + concentration were 0.9839, 4.34 and 4.62 mmol/l, respectively. These results suggested that the model had an accurate predictive capacity for NH4 + concentration. The proposed model provides a potential fast way for control and optimization of glutamate fermentation process.
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References
Nandakumar R, Yoshimune K, Wakayama M et al (2003) Microbial glutaminase: biochemistry, molecular approaches and applications in the food industry. J Mol Catal B-Enzym 23:87–100
Jyothi A, Sasikiran K, Nambisan B et al (2005) Optimisation of glutamic acid production from cassava starch factory residues using Brevibacterium divaricatum. Process Biochem 40:3576–3579
Kusumoto I (2001) Industrial production of l-glutamine. J Nutr 131:2552S–2555S
Kinoshita S (1985) Glutamic acid bacteria. In: Demain, Solomon (eds) Biology of industrial microorganisms. Wiley, London, pp 115–142
Shimizu H, Hirasawa T (2007) Production of glutamate and glutamate-related amino acids: molecular mechanism analysis and metabolic engineering. In: Wendisch Volker F (ed) Amino acid biosynthesis-pathways. Regulation and Metabolic Engineering, Wiley, pp 1–38
Momose H, Takagi T (1978) Glutamic acid production in biotin-rich media by temperature sensitive mutants of Brevibacterium lactofermentum, a novel fermentation process. Agr Biol Chem 42:1911–1917
Delaunay S, Gourdon P, Lapujade P et al (1999) An improved temperature-triggered process for glutamate production with Corynebacterium glutamicum. Enzyme Microb Tech 25:762–768
Eggeling L, Bott M (2005) Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, pp 439–463
Hashimoto KI, Kawasaki H, Akazawa K et al (2006) Changes in composition and content of mycolic acids in glutamate-overproducing Corynebacterium glutamicum. Biosci Biotech Biochem 70:22–30
Bokas D, Grattepanche F, Duportail G et al (2007) Cell envelope fluidity modification for an effective glutamate excretion in Corynebacterium glutamicum 2262. Appl Microbiol Biot 76:773–781
Tesch M, Eikmanns B J, Graaf AA et al (1998) Ammonia assimilation in Corynebacterium glutamicum and a glutamate dehydrogenase-deficient mutant. Biotech Lett 20:953–957
Nicolai BM, Beullens K, Bobelyn E et al (2007) Nondestructive measurement of fruit and vegetable quality by means of NIR spectroscopy: a review. Postharvest Biol Tec 46:99–118
Aït Kaddour A, Mondet M, Cuq B (2008) Application of two-dimensional cross-correlation spectroscopy to analyse infrared (MIR and NIR) spectra recorded during bread dough mixing. J Cereal Sci 48:678–685
Shiroma C, Rodriguez-Saona L (2009) Application of NIR and MIR spectroscopy in quality control of potato chips. J Food Compos Anal 22:596–605
Krämer K, Ebel S (2000) Application of NIR reflectance spectroscopy for the identification of pharmaceutical excipients. Anal Chim Acta 420:155–161
De Maesschalck R, Sanchez FC, Massart DL et al (1998) On-line monitoring of powder blending with near-infrared spectroscopy. Appl Spectrosc 52:725–731
Amigo J, Cruz JM, Bautista M et al (2008) Study of pharmaceutical samples by NIR chemical-image and multivariate analysis. Trac-Trend Anal Chem 27:696–713
Fernández-Novales J, López MI, Sánchez MT et al (2008) A feasibility study on the use of a miniature fiber optic NIR spectrometer for the prediction of volumic mass and reducing sugars in white wine fermentations. J Food Eng 89:325–329
Zeaiter M, Roger JM, Bellon-Maurel V (2006) Dynamic orthogonal projection. A new method to maintain the on-line robustness of multivariate calibrations. Application to NIR-based monitoring of wine fermentations. Chemometr Intell Lab 80:227–235
Yu HY, Niu XY, Lin HJ et al (2009) A feasibility study on on-line determination of rice wine composition by Vis–NIR spectroscopy and least-squares support vector machines. Food Chem 113:291–296
Blanco M, Villarroya I (2002) NIR spectroscopy: a rapid-response analytical tool. Trac-Trend Anal Chem 21:240–250
Puchert T, Holzhauer CV, Menezes JC et al (2011) A new PAT/QbD approach for the determination of blend homogeneity: combination of on-line NIRS analysis with PC scores distance analysis (PC-SDA). Eur J Pharm Biopharm 78:173–182
Thomas EV (2000) Adaptable multivariate calibration models for spectral applications. Anal Chem 72:2821–2827
Spiegelman CH, McShane MJ, Goetz MJ et al (1998) Theoretical justification of wavelength selection in PLS calibration: development of a new algorithm. Anal Chem 70:35–44
Cozzolino D, Kwiatkowski MJ, Parker M et al (2004) Prediction of phenolic compounds in red wine fermentations by visible and near infrared spectroscopy. Anal Chim Acta 513:73–80
Acknowledgments
This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (IRT 1166), by Tianjin Research Program of science and technology (Grant No. 12ZCZDSY01900), national high technology research and development program (2013AA102106), and by national science and technology supporting program (2011BAC11BB03).
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Liang, J. et al. (2014). Prediction of NH4 + Concentration During the Temperature Triggered Glutamate Fermentation Using At-Line Near-Infrared Spectroscopy. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_171
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DOI: https://doi.org/10.1007/978-3-642-37925-3_171
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