Abstract
Saccharomyces cerevisiae is capable of fermenting galactose into ethanol, but the productivity from galactose is much lower than those from glucose. An effective approach is undertaken to improve galactose utilized ability and ethanol productivity through gene engineering of the regulatory network controlling the expression of the GAL genes. The GAL gene regulatory network of S. cerevisiae is a tightly regulated system. Gal6, Gal80, and Mig1 are three known negative regulators of the GAL system. In this paper, Gal6, Gal80, and Mig1 were knockout by the way of homologous recombination. This led to a 76 % increase in specific galactose uptake rate compared with the wild-type strain. And the ethanol yield has advanced greatly. Further study showed that GAL80 and MIG1 played more important roles in galactose fermentation of S. cerevisiae than GAL6 did.
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Acknowledgments
This work was financially supported by the program of National High Technology Research and Development Program of China (863 Program) (Grant No. SS2012AA023408), the Cheung Kong Scholars, and Innovative Research Team Program in University of Ministry of Education, China (Grant No. IRT1166), and Application Base and Frontier Technology Project of Tianjin, China (Grant No. 09JCZDJC17900). The work is supported by the “2012–2013” Foundation of Laboratory of Tianjin University of Science and Technology, P.R. China (Grant No. 1204A209).
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Shen, T., Guo, X., Zou, J., Li, Y., Ma, J., Xiao, D. (2014). Increasing Galactose Utilized Ability of Saccharomyces cerevisiae Through Gene Engineering. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_22
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DOI: https://doi.org/10.1007/978-3-642-37916-1_22
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