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Aptamers: Analytical Tools for Viral Components

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DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases

Part of the book series: RNA Technologies ((RNATECHN))

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Abstract

Aptamers are oligonucleotides obtained by a combinatorial method from randomly synthesized libraries. They usually exhibit strong affinity and high specificity of interaction with a predetermined target. They actually rival antibodies and are of interest for multiple purposes, notably for diagnostic. As aptamers can be easily immobilized on a number of surfaces, they can be used for detecting the presence of viral constituents, RNA or proteins. The detection can be carried out in a number of different formats (fluorescence, SPR, etc.). We raised aptamers against RNA motifs of the human immunodeficiency virus or of the hepatitis C virus. Such aptamers display a very high specificity of recognition, surpassing that of complementary oligonucleotides as they recognize the 3D shape and not only the primary sequence. We also raised aptamers against the M1 protein of the influenza virus allowing its detection. We took advantage of fluorescence transfer for monitoring interactions between aptamers and their cognate ligand immobilized on particles. This strategy could be used either for screening libraries or for evaluating the presence of an analyte.

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Acknowledgments

We thank Dr Sonia Da Rocha, Mrs Laetitia Evadé, and Ms Emilie Daguerre (Bordeaux) for sharing unpublished results.

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Authors and Affiliations

  1. ARNA Laboratory, INSERM U869, European Institute of Chemistry and Biology, 2 rue Robert Escarpit, 33607, Pessac, France

    Jean-Jacques Toulmé, Carmelo Di Primo, Eric Dausse & Laurent Azéma

  2. ARNA Laboratory, European Institute of Chemistry and Biology, University of Bordeaux, 33076, Bordeaux, France

    Jean-Jacques Toulmé, Carmelo Di Primo, Eric Dausse & Laurent Azéma

  3. Department of Pathology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium

    Daniel Desmecht & François Cornet

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  1. Jean-Jacques Toulmé
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  2. Carmelo Di Primo
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  3. Eric Dausse
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  4. Daniel Desmecht
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  5. François Cornet
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  6. Laurent Azéma
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Correspondence to Jean-Jacques Toulmé .

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Editors and Affiliations

  1. Institute of Chemistry/Biochemistry, Free University Berlin, Thielallee 63, Berlin, 14195, Berlin, Germany

    Volker A. Erdmann

  2. Inst. Bioorganic Chemistry, PAN Poznan, ul. Z. Noskowskiego 12/14, Poznan, 61-704, Poland

    Jan Barciszewski

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Toulmé, JJ., Primo, C.D., Dausse, E., Desmecht, D., Cornet, F., Azéma, L. (2013). Aptamers: Analytical Tools for Viral Components. In: Erdmann, V., Barciszewski, J. (eds) DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases. RNA Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36853-0_17

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