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Immunophenotyping in Myelodysplastic Syndromes

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Myelodysplastic Syndromes

Part of the book series: Hematologic Malignancies ((HEMATOLOGIC))

Abstract

Myelodysplastic syndromes (MDS) comprise a heterogeneous group of clonal hematological disorders that is diagnosed cytomorphologically. They are characterized by ineffective hematopoiesis resulting in peripheral blood cytopenias and showing by definition in all cases dysplastic features in at least one lineage in the bone marrow, an increased myeloid blast count in the bone marrow or typical chromosomal aberrations. They all share a variable risk for transformation into an acute myeloid leukemia.

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References

  • Alhan C, Westers TM, Cali C, Ossenkoppele GJ, van de Loosdrecht AA (2011) Validation of a flow cytometric scoring system for the prognostication of the myelodysplastic syndromes; a retrospective cohort study. In: The 11th international symposium on myelodysplastic syndromes, Edinburgh, p 250, 18–21 May 2011

    Google Scholar 

  • Bennett JM, Catovsky D, Daniel MT et al (1982) Proposals for the classification of the myelodysplastic syndromes. Br J Haematol 51(2):189–199

    PubMed  CAS  Google Scholar 

  • Borowitz MJ, Guenther KL, Shults KE, Stelzer GT (1993) Immunophenotyping of acute leukemia by flow cytometric analysis. Use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color analysis. Am J Clin Pathol 100(5):534–540

    PubMed  CAS  Google Scholar 

  • Brunning RD, Orazi A, Germing U et al (2008) Myelodysplastic syndromes/neoplasms, overview. In: Swerdlow SH, Campo E, Harris NL (eds) WHO classification of tumours of haematopoietic and lymphoid tissues, 4th edn. International Agency for Research on Cancer (IARC), Lyon, pp 88–93

    Google Scholar 

  • Della Porta MG, Picone C, Pascutto C et al (2012) Multicentre validation of a reproducible flow cytometric score for the diagnosis of low-risk myelodysplastic syndromes: results of a European LeukemiaNET study. Haematologica 97(8):1209–1217

    Article  PubMed  Google Scholar 

  • Goardon N, Nikolousis E, Sternberg A et al (2009) Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica 94(8):1160–1163

    Article  PubMed  Google Scholar 

  • Greenberg P, Cox C, Le Beau MM et al (1997) International Scoring System for evaluating prognosis in myelodysplastic syndromes. Blood 89(6):2079–2088

    PubMed  CAS  Google Scholar 

  • Greenberg P, Tuechler H, Schanz J et al (2011) Revised International Prognostic Scoring System (IPSS-R), developed by the International Prognostic Working Group for Prognosis in MDS (IWG-PM). In: The 11th international symposium on myelodysplastic syndromes, Edinburgh, p 274, 18–21 May 2011

    Google Scholar 

  • Jaffe ES (2001) Pathology and genetics of tumours of haematopoietic and lymphoid tissues, WHO classification of tumours. IARC Press, Lyon

    Google Scholar 

  • Kern W, Haferlach C, Schnittger S, Haferlach T (2010) Clinical utility of multiparameter flow cytometry in the diagnosis of 1013 patients with suspected myelodysplastic syndrome: correlation to cytomorphology, cytogenetics, and clinical data. Cancer 116(19):4549–4563

    Article  PubMed  Google Scholar 

  • Kern W, Haferlach C, Schnittger S, Haferlach T (2011) Clinical impact of multiparameter flow cytometry in diagnosing myelodysplastic syndromes. In: The 11th international symposium on myelodysplastic syndromes, Edinburgh, p 361, 18–21 May 2011

    Google Scholar 

  • Malcovati L, Della Porta MG, Lunghi M et al (2005) Flow cytometry evaluation of erythroid and myeloid dysplasia in patients with myelodysplastic syndrome. Leukemia 19(5):776–783

    Article  PubMed  CAS  Google Scholar 

  • Mufti GJ, Stevens JR, Oscier DG, Hamblin TJ, Machin D (1985) Myelodysplastic syndromes: a scoring system with prognostic significance. Br J Haematol 59(3):425–433

    Article  PubMed  CAS  Google Scholar 

  • Ogata K (2011) Flow cytometry will be a routine tool in clinical practice in myelodysplastic syndromes: a real story. Leuk Res 35(7):848–849

    Article  PubMed  Google Scholar 

  • Ogata K, Nakamura K, Yokose N et al (2002) Clinical significance of phenotypic features of blasts in patients with myelodysplastic syndrome. Blood 100(12):3887–3896

    Article  PubMed  CAS  Google Scholar 

  • Ogata K, la Porta MG, Malcovati L et al (2009) Diagnostic utility of flow cytometry in low-grade myelodysplastic syndromes: a prospective validation study. Haematologica 94(8):1066–1074

    Article  PubMed  Google Scholar 

  • Ossenkoppele GJ, van de Loosdrecht AA, Schuurhuis GJ (2011) Review of the relevance of aberrant antigen expression by flow cytometry in myeloid neoplasms. Br J Haematol 153(4):421–436

    Article  PubMed  CAS  Google Scholar 

  • Scott BL, Wells DA, Loken MR et al (2008) Validation of a flow cytometric scoring system as a prognostic indicator for posttransplantation outcome in patients with myelodysplastic syndrome. Blood 112(7):2681–2686

    Article  PubMed  CAS  Google Scholar 

  • Stetler-Stevenson M, Arthur DC, Jabbour N et al (2001) Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome. Blood 98(4):979–987

    Article  PubMed  CAS  Google Scholar 

  • Swerdlow SH, Campo E, Harris NL et al (2008) WHO classification of tumours of haematopoietic and lymphoid tissues. International Agency for Research on Cancer (IARC), Lyon

    Google Scholar 

  • van de Loosdrecht AA, Westers TM (2011) Flow cytometry in myelodysplastic syndromes: ready for translation into clinical practice. Leuk Res 35(7):850–852

    Article  PubMed  Google Scholar 

  • van de Loosdrecht AA, Westers TM, Westra AH et al (2008) Identification of distinct prognostic subgroups in low- and intermediate-1-risk myelodysplastic syndromes by flow cytometry. Blood 111(3):1067–1077

    Article  PubMed  Google Scholar 

  • van de Loosdrecht AA, Alhan C, Bene MC et al (2009) Standardization of flow cytometry in ­myelodysplastic syndromes: report from the first European LeukemiaNet working conference on flow cytometry in myelodysplastic syndromes. Haematologica 94(8):1124–1134

    Article  PubMed  Google Scholar 

  • Wells DA, Benesch M, Loken MR et al (2003) Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with the IPSS and with outcome after hematopoietic stem cell transplantation. Blood 102(1):394–403

    Article  PubMed  CAS  Google Scholar 

  • Westers TM, Alhan C, Chamuleau ME et al (2010) Aberrant immunophenotype of blasts in myelodysplastic syndromes is a clinically relevant biomarker in predicting response to growth factor treatment. Blood 115(9):1779–1784

    Article  PubMed  CAS  Google Scholar 

  • Westers TM, Ireland R, Kern W et al (2012) Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group. Leukemia 26(7):1730–1741

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. MLL Munich Leukemia Laboratory, Max-Lebsche-Platz 31, 81377, Munich, Germany

    Wolfgang Kern MD

  2. Department of Hematology, Cancer Center Amsterdam, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

    Arjan A. van de Loosdrecht MD, PhD

Authors
  1. Wolfgang Kern MD
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  2. Arjan A. van de Loosdrecht MD, PhD
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Correspondence to Wolfgang Kern MD .

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© 2013 Springer-Verlag Berlin Heidelberg

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Kern, W., van de Loosdrecht, A.A. (2013). Immunophenotyping in Myelodysplastic Syndromes. In: Myelodysplastic Syndromes. Hematologic Malignancies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36229-3_9

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  • DOI: https://doi.org/10.1007/978-3-642-36229-3_9

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-36228-6

  • Online ISBN: 978-3-642-36229-3

  • eBook Packages: MedicineMedicine (R0)

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