Abstract
Culture independent metagenomic methods represent promising approach for the identification of novel genes and genomes of uncultivable microorganisms. A wide range of protocols have been developed for the isolation of community DNA from environmental sources. Three important steps in DNA isolation from soil are (a) cell lysis, (b) removal of contaminants, in particular humic acid, and (c) purification of isolated community DNA. Depending on the nature of the environmental DNA source, modifications are usually performed in any of the above steps. The steps involved in the isolation of DNA and RNA from soil samples and the appropriate purification strategies are described in this chapter. Additionally, protocols for the microbial diversity analysis using 16S ribosomal RNA analysis and construction of metagenomic library for screening of functional genes from metagenomic DNA are given.
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Rajesh, T., Rajendhran, J., Gunasekaran, P. (2013). Analysis of Microbial Diversity and Construction of Metagenomic Library. In: Arora, D., Das, S., Sukumar, M. (eds) Analyzing Microbes. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34410-7_14
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DOI: https://doi.org/10.1007/978-3-642-34410-7_14
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