Abstract
The main method was an intravascular injection of India ink. This method permits the vascular anatomy to be observed in different ways:
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Observation of the superficial (leptomeningeal) arteries and veins. Their respective points of penetration or emergence are noted.
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Identification of the deep patterns of intrahippocampal arteries and veins.
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Thick sections (300-μm thick) were obtained into coronal, sagittal, and axial sections and cleared using the Spalteholz technique, allowing the identification of cerebral structures according to their difference in vascular density.
Bodian’s method of silver impregnation was performed after intravascular injection, and a correlation can thus be established between the neural architecture and the vascular network.
Intravascular injection with low-viscosity resin (Mercox) permits the fine and precise view of the microvascularization after tissue destruction and examination with a scanning electron microscope (SEM).
The last part of this work is devoted to sectional anatomy and 3T magnetic resonance imaging.
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Duvernoy, H., Cattin, F., Risold, PY. (2013). Material and Methods. In: The Human Hippocampus. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-33603-4_2
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DOI: https://doi.org/10.1007/978-3-642-33603-4_2
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